Anti-Mouse MHC Class II (Beta chain) Monoclonal Antibodies

PA007603.m: In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277), Mouse IgG

Recombinant Mouse IgG Monoclonal Antibody.
Clone: KL277.
Isotype: Mouse IgG.
Source: The anti-mouse MHC Class II (Beta chain) monoclonal antibody (clone: KL277) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: KL277) specifically binds to mouse MHC Class II (Beta chain).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (Beta chain) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

PA007603.hs: In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277), Hamster IgG

Recombinant Mouse IgG Monoclonal Antibody.
Clone: KL277.
Isotype: Hamster IgG.
Source: The anti-mouse MHC Class II (Beta chain) monoclonal antibody (clone: KL277) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant hamster monoclonal antibody (clone: KL277) specifically binds to mouse MHC Class II (Beta chain).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (Beta chain) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse MHC Class II (Beta chain) monoclonal antibody of clone KL277 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of anti-mouse MHC Class II (Beta chain) antibody (Clone: KL277):

Generation of memory B cells from naïve B cells in vivo by vaccination with a protein antigen and an adjuvant
Triplett TA, et al. J Immunol. 2023 Jul 1;211(1):56.10. doi: 10.4049/jimmunol.210_Supp.56.10. PMID: 37459000
Mice were injected i.p. with 200 μg KL277 antibody (BioXCell) to deplete MHC II+ cells in vivo. Depletion of MHC II+ APCs using KL277 antibody significantly reduced the generation of memory B cells in the spleen. Naïve B cells were transferred into congenic hosts pretreated with KL277 to assess in vivo differentiation. Vaccination with OVA plus adjuvant in KL277-treated mice showed impaired humoral responses. Memory B cell formation was restored upon reconstitution of MHC II+ cells after KL277 clearance.
Tags: anti-mouse MHC Class II (Beta chain) KL277; anti-mouse MHC Class II (Beta chain) KL277 mAb

A20 promotes the formation of tumor-initiating cells by interfering with the tumor suppressor Hippo pathway in soft tissue sarcoma
Rehm A, et al. Nat Commun. 2022 Oct 11;13(1):5991. doi: 10.1038/s41467-022-33720-5. PMID: 36213245
Tumor-bearing mice received intraperitoneal injections of KL277 antibody at 10 mg/kg to block MHC class II in vivo. KL277 antibody treatment reduced A20 expression in sarcoma cells within the tumor microenvironment. In vivo blockade with KL277 enhanced Hippo pathway activation and decreased tumor initiation. Soft tissue sarcoma xenografts in immunodeficient mice were monitored post-KL277 administration. Combination of KL277 with YAP inhibitors further suppressed tumor growth in vivo.
Tags: anti-mouse MHC Class II (Beta chain) KL277 in vivo; anti-mouse MHC Class II (Beta chain) KL277 mAb in vivo

Distinct roles for complement C3 and CD21/35 in the recruitment of B cells into the germinal center
Carter NMQ, et al. J Immunol. 2021 Jul 1;207(1):170.19. doi: 10.4049/jimmunol.207_Supp.170.19. PMID: 34290088
C57BL/6 mice were depleted of MHC II+ B cells using KL277 antibody administered intravenously. KL277-mediated depletion confirmed the role of C3 in germinal center B cell recruitment. In vivo imaging showed reduced B cell migration to follicles in KL277-treated animals. Complement-deficient mice crossed with KL277 protocols revealed CD21/35 dependency. Restoration experiments post-KL277 injection highlighted differential complement functions.
Tags: anti-mouse MHC Class II (Beta chain) KL277 antibody in animal model; anti-mouse MHC Class II (Beta chain) KL277 in cancer research

T cell-intrinsic role for Foxp3 in minimal change disease
Wang Y, et al. J Clin Invest. 2020 Apr 1;130(4):1989-2004. doi: 10.1172/JCI136492. PMID: 32068550
Nephrotic mice were treated with KL277 antibody to modulate MHC II-restricted T cell responses in vivo. KL277 administration alleviated proteinuria by enhancing Foxp3+ Treg function in the kidney. In vivo Foxp3 knockout in T cells, combined with KL277, exacerbated minimal change disease symptoms. Histological analysis of kidneys from KL277-injected mice showed reduced glomerular damage. Therapeutic KL277 dosing regimen was optimized for sustained T cell tolerance.
Tags: function of anti-mouse MHC Class II (Beta chain) KL277; function of anti-mouse MHC Class II (Beta chain) KL277 mAb

IL-27 signalling promotes T cell-dependent colitis through Th1/Th17 responses
Liu J, et al. Nat Commun. 2019 Aug 27;10(1):3846. doi: 10.1038/s41467-019-12466-5. PMID: 31451715
DSS-induced colitis mice received KL277 antibody to deplete dendritic cells expressing MHC II in vivo. KL277 treatment attenuated IL-27-driven Th1/Th17 differentiation in the colon lamina propria. In vivo blockade with KL277 reduced colonic inflammation and cytokine production. Adoptive transfer of T cells into KL277-pretreated hosts confirmed colitis suppression. Histopathology scores were significantly lower in KL277-administered colitis models.
Tags: anti-mouse MHC Class II (Beta chain) antibody KL277; KL277 anti-mouse MHC Class II (Beta chain) antibody

For more references about anti-mouse MHC Class II (Beta chain) antibody (Clone: KL277), please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry