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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: L243.
Isotype: Mouse IgG2a Kappa.
Source: The anti-human HLA-DR monoclonal antibody (clone: L243) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant L243 antibody specifically binds to human HLA-DR.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: L243.
Isotype: Human IgG1 kappa.
Source: The anti-human HLA-DR monoclonal antibody (clone: L243) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant L243 antibody specifically binds to human HLA-DR.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The L243 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Identifying major histocompatibility complex class II-DR molecules in bovine and swine peripheral blood monocyte-derived macrophages using mAb-L243
Celis-Giraldo, C., et al. (2024). Vaccine. 2024 May 31. doi: 10.1016/j.vaccine.2024.04.042. PMID: 38631956
The L243 monoclonal antibody (anti-human HLA-DR) was used to identify MHC-II-DR molecules in bovine and swine macrophages via flow cytometry, mass spectrometry, and immunopurification. The study demonstrated cross-reactivity of L243 with bovine and swine DR class II molecules, validating its use for immunopeptidomics to identify Staphylococcus aureus and Salmonella enterica peptides presented by MHC-II-DR.
Tags: activity of L243 antibody; activity of L243 mAb
HLA class II antibodies induce necrotic cell death in human endothelial cells via a lysosomal membrane permeabilization-mediated pathway
Aljabri, A., et al. (2019). Cell Death Dis. 2019 Mar 8. doi: 10.1038/s41419-019-1319-5. PMID: 30850581
The L243 antibody (anti-human HLA-DR) induced cell death in IFN-γ-stimulated human endothelial cells after 3 hours, as shown by annexin V/propidium iodide and LIVE/DEAD staining. Preincubation with soluble recombinant HLA-DR confirmed L243’s specific binding to HLA-DR, demonstrating its cytotoxic effect in the absence of complement, unlike its proliferative effect after 48 hours.
Tags: function of L243 mAb; L243 antibody
Combating non-Hodgkin lymphoma by targeting both CD20 and HLA-DR through CD20–243 CrossMab
Zhao, L., et al. (2014). MAbs. 2014 May-Jun;6(3):740-8. doi: 10.4161/mabs.28613. PMID: 24670986
The L243 antibody (anti-human HLA-DR) served as a positive control for HLA-DR binding in flow cytometry, showing comparable binding affinity to CD20-243 CrossMab on Raji cells. L243 competition confirmed specific HLA-DR epitope binding, with flow cytometry revealing enhanced internalization upon co-engagement of CD20 and HLA-DR.
Tags: L243 antibody for flow cytometry; L243 antibody for mouse tumor model
A naturally processed HLA-DR-bound peptide from the IL-9 receptor alpha of HTLV-1-transformed T cells serves as a T helper epitope
Kobayashi, H., et al. (2012). Cancer Immunol Immunother. 2012 Dec. doi: 10.1007/s00262-012-1284-7. PMID: 22638550
The L243 antibody (anti-human HLA-DR) was used to immunoprecipitate HLA-DR molecules from HTLV-1-transformed T cells, with eluted peptides analyzed by mass spectrometry. Flow cytometry with L243 confirmed high HLA-DR surface expression, and L243 blocked T cell responses, verifying HLA-DR-restricted presentation of an IL-9 receptor alpha epitope.
Tags: L243 antibody mouse dose range; L243 mAb for animal model
Characterization of a humanized IgG4 anti-HLA-DR monoclonal antibody that lacks effector cell functions but retains direct antilymphoma activity and increases the potency of rituximab
Stein, R., et al. (2006). Blood. 2006 Oct 15. doi: 10.1182/blood-2006-04-017921. PMID: 16778139
The L243 antibody (anti-human HLA-DR), among other murine anti-HLA class II mAbs, demonstrated antibody-dependent cellular cytotoxicity (ADCC) in lymphoma cells. A humanized IgG4 version of L243 retained direct antilymphoma activity without effector functions, enhancing rituximab’s potency in targeting HLA-DR-expressing cells.
Tags: L243 mAb for mouse tumor model; L243 monoclonal antibody
For more references about anti-human HLA-DR antibody (L243), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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