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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human HLA-ABC Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells.
Clone: W6/32.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: W6/32) specifically binds to human HLA-ABC.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human HLA-ABC protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human HLA-ABC antibodies (clone of W6/32) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
ALK and RET Inhibitors Promote HLA Class I Antigen Presentation and Unmask New Antigens within the Tumor Immunopeptidome
Oh CY, et al. Cancer Immunol Res. 2019 Dec;7(12):1984-1997. doi: 10.1158/2326-6066.CIR-19-0056. PMID: 31554664
This study investigates the effects of ALK and RET inhibitors on HLA class I antigen presentation in tumor-bearing mice. Surface HLA class I levels were measured by flow cytometry using the W6/32 antibody (anti-human HLA-ABC). Tumors were treated with ALK inhibitors (ceritinib or crizotinib) or RET inhibitor (selpercatinib) daily for 14 days, then processed into single-cell suspensions for analysis. Peptide-MHC complexes were immunoprecipitated using W6/32-coupled beads, and mass spectrometry identified novel peptides presented on HLA class I post-treatment.
Tags: anti-human HLA-ABC W6/32; anti-human HLA-ABC W6/32 mAb
Mechanism of accommodation in a sensitized HLA transgenic murine cardiac transplant model
Fukami N, et al. Transplantation. 2012 Feb 27;93(4):423-8. doi: 10.1097/TP.0b013e3182419810. PMID: 22210658
This study explores the mechanism of accommodation in a sensitized HLA transgenic murine cardiac transplant model. Donor pretreatment involved three doses of W6/32 antibody (anti-human HLA-ABC, 1 mg/kg) administered intraperitoneally on days -7, -5, and -3 before transplantation. Cardiac allografts from HLA-A2 transgenic mice were transplanted into C57BL/6 recipients. Graft survival was monitored by daily palpation, and histological analysis was performed using hematoxylin and eosin staining. Flow cytometry confirmed upregulation of anti-apoptotic genes in donor hearts post-W6/32 treatment.
Tags: anti-human HLA-ABC W6/32 mAb in vivo; anti-human HLA-ABC W6/32 antibody in animal model
HLA class I antibody-mediated endothelial and smooth muscle cell activation through macrophages and platelets
Zhang X, et al. J Immunol. 2013 Jan 15;190(2):759-67. doi: 10.4049/jimmunol.1200137. PMID: 23263599
This study examines HLA class I antibody-mediated activation of endothelial and smooth muscle cells in HLA-A2 transgenic mice. Low-dose W6/32 antibody (anti-human HLA-ABC, 0.1 mg/kg) was administered intraperitoneally to induce endothelial activation. Human umbilical vein endothelial cells were cultured with HLA class I antibodies to mimic in vivo conditions. Platelet activation was assessed by P-selectin expression via flow cytometry, and macrophage infiltration was quantified by immunohistochemistry. Graft survival was significantly prolonged in W6/32-pretreated mice compared to controls.
Tags: anti-human HLA-ABC W6/32 in cancer research; anti-human HLA-ABC W6/32 antibody in cancer research
Antibodies to MHC class I induce autoimmunity: role in the pathogenesis of chronic rejection
Fukami N, et al. J Immunol. 2009 Dec 15;183(12):7921-7. doi: 10.4049/jimmunol.0901359. PMID: 19949365
This study investigates the role of MHC class I antibodies in inducing autoimmunity and chronic rejection in HLA-A2 transgenic mice. W6/32 antibody (anti-human HLA-ABC, 1 mg/kg) was administered intratracheally, leading to epithelial hyperplasia and fibrosis. Lung allografts were harvested at day 30 for histological and immunohistochemical analysis. Serum autoantibody levels were measured by ELISA, and flow cytometry confirmed increased MHC class I expression on airway epithelial cells, with chronic rejection features like obliterative bronchiolitis observed.
Tags: function of anti-human HLA-ABC W6/32; function of anti-human HLA-ABC W6/32 mAb
Human-like mouse models for testing the efficacy and safety of anti-β2-microglobulin monoclonal antibodies to treat myeloma
Yang J, et al. Clin Cancer Res. 2009 Mar 15;15(6):951-9. doi: 10.1158/1078-0432.CCR-08-1855. PMID: 19265077
This study evaluates anti-β2-microglobulin monoclonal antibodies in NOD/SCID mice with myeloma xenografts established using human MM1.S cells. Splenocytes were cross-linked with W6/32 antibody (anti-human HLA-ABC) to assess MHC class I signaling pathways. Anti-β2-microglobulin antibodies were administered intravenously at 10 mg/kg weekly. Tumor burden was monitored by bioluminescence imaging twice weekly, and flow cytometry confirmed MHC class I expression on myeloma cells using W6/32 staining.
Tags: anti-human HLA-ABC W6/32 antibody of low endotoxin; clone W6/32 of anti-human HLA-ABC
For more references about anti-human HLA-ABC antibody (W6/32), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2a isotype control antibody
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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