Name: GM-CSF Protein
Brand: Syd Labs
SKU: BP000176-GD19
Price: 250.00 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No.

Product Name

Size

List Price (US$)

Quantity

BP000176-GD19

Recombinant Human GM-CSF Protein

50 ug

250.00

BP000176-GD19

Recombinant Human GM-CSF Protein

300 ug

500.00

BP000176-GD19

Recombinant Human GM-CSF Protein

1 mg

1250.00

Description

Introduction
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was first characterized as a growth factor that supports the in-vitro colony formation of granulocytes-macrophages progenitor cells. It is a pleiotropic cytokine and a member of a family of endogenous cytokines of the hematopoietic system. GM-CSF is produced as a response to immune or inflammatory stimuli by activated cells of the hematopoietic system such as T cells, B cells, macrophages, mast cells and also fibroblasts and alveolar epithelial cells. It plays an important role in regulating the proliferation, differentiation, survival and activation of hematopoietic cells such as granulocytes and monocytes, neutrophiles, basophiles and eosonophoiles, erythroid cells, megakaryocytes, and T cells. Bacterial endotoxins, TNF, Interleukin-1, and GM-CSF induce the synthesis of G-CSF.

BP000176-GD19: Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Protein

Source: E. coli-derived.
Recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) protein is expressed with sequence (A18-E144) of human GM-CSF. It is a 14.6 kDa globular protein consisting of 128 amino acids (with the N-terminal methionine) containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.
N-terminal sequence analysis: (Met)-Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr-Gln-Pro-Trp-Glu-His.
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10^7 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.01 ng/ug (0.1 IEU/ug).
Formulation: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 M NaCl, 5% Mannitol, pH 7.2.
Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

References of GM-CSF Protein:

GM-CSF: Master regulator of the T cell-phagocyte interface during inflammation
Hamilton JA, et al. Semin Immunol. 2021 Aug;54:101514. doi: 10.1016/j.smim.2021.101514. PMID: 34052218
Human recombinant GM-CSF protein was used to stimulate peripheral blood mononuclear cells (PBMCs) at 10 ng/mL for 24 hours. GM-CSF protein levels in synovial fluid were elevated in rheumatoid arthritis (RA) patients compared to controls. The addition of neutralizing anti-GM-CSF antibody reduced cytokine production induced by GM-CSF protein. In vivo administration of GM-CSF protein exacerbated autoimmune encephalitis in mice. GM-CSF protein signaling via the STAT5 pathway promotes monocyte differentiation.
Tags: recombinant GM-CSF protein; GM-CSF protein supplier

Evaluation of the effect of GM-CSF blocking on the phenotype and function of human monocytes
Herbst T, et al. Sci Rep. 2020 Feb 21;10(1):3129. doi: 10.1038/s41598-020-58131-2. PMID: 32092100
Human GM-CSF protein (20 ng/mL) was added to monocyte cultures for 7 days. Blocking GM-CSF protein with lenzilumab altered monocyte gene expression profile. CXCL11 mRNA was upregulated in response to GM-CSF protein stimulation. Flow cytometry showed increased CD14 expression on monocytes treated with GM-CSF protein. GM-CSF protein blockade reduced TNF-alpha secretion from activated monocytes.
Tags: buy Human GM-CSF Protein; low endotoxin GM-CSF protein

GM-CSF-based treatments in COVID-19: reconciling opposing therapeutic approaches
Mehta P, et al. Nat Rev Immunol. 2020 Jul;20(7):448-451. doi: 10.1038/s41577-020-0357-7. PMID: 32488013
Recombinant human GM-CSF protein is being tested in phase II trials for acute respiratory distress syndrome (ARDS) in COVID-19. GM-CSF protein acts as both a growth factor and pro-inflammatory cytokine in lung pathology. Administration of GM-CSF protein may exacerbate cytokine storm in severe cases. Neutralizing anti-GM-CSF protein antibodies are proposed to dampen hyperinflammation. Clinical trials with GM-CSF protein fusion constructs show mixed outcomes in respiratory failure.
Tags: E. coli expressed GM-CSF protein; human GM-CSF protein

GM-CSF: From Growth Factor to Central Mediator of Tissue Inflammation
Becher B, et al. Immunity. 2016 Dec 20;45(6):1140-1151. doi: 10.1016/j.immuni.2016.10.034. PMID: 27890464
Human GM-CSF protein induces dendritic cell maturation in vitro at picomolar concentrations. In chronic inflammation, GM-CSF protein bridges innate and adaptive immunity. Knockout of GM-CSF protein in mice leads to impaired alveolar macrophage function. Therapeutic targeting of GM-CSF protein is explored in multiple sclerosis models. GM-CSF protein expression is upregulated by TLR signaling in macrophages.
Tags: activity of GM-CSF protein; bulk GM-CSF protein

Neutralization and clearance of GM-CSF by autoantibodies in pulmonary alveolar proteinosis
Picatoste B, et al. Nat Commun. 2015 Jun 16;6:8375. doi: 10.1038/ncomms9375. PMID: 26083147
Recombinant human GM-CSF protein was used in ELISA to detect autoantibodies in pulmonary alveolar proteinosis (PAP) sera. Autoantibodies neutralize GM-CSF protein activity, leading to impaired surfactant clearance. Bronchoalveolar lavage (BAL) fluid from PAP patients showed undetectable levels of bioactive GM-CSF protein. Affinity purification confirmed high-affinity binding of autoantibodies to GM-CSF protein. Restoration of GM-CSF protein function via inhalation therapy improves lung function.
Tags: function of GM-CSF protein; GM-CSF protein dose range

For more references about GM-CSF Protein please contact our scientific support team with message@sydlabs.com.

Other GM-CSF Proteins:
Recombinant human GM-CSF protein with His-tag
Recombinant human GM-CSF protein produced in HEK
Recombinant human GM-CSF protein produced in SF9
Recombinant human GM-CSF protein produced in yeast
Recombinant mouse GM-CSF protein
Recombinant rat GM-CSF protein
Recombinant porcine GM-CSF protein
Recombinant monkey GM-CSF protein
Recombinant canine GM-CSF protein

The recombinant human GM-CSF protein expressed in E. coli, such as molgramostin, is non-glycosylated, different from the natural glycoprotein and the recombinant human GM-CSF protein expressed in yeast cells, such as sargramostim.

GM-CSF Antibodies:
GM-CSF polyclonal antibody
GM-CSF monoclonal antibody

GM-CSF ELISA Kits:
Rat GM-CSF ELISA kit
Mouse GM-CSF ELISA kit

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GM-CSF Protein

Granulocyte Macrophage Colony Stimulating Factor, MGI-1GM, Pluripoietin-alpha, Molgramostin, Sargramostim, CSF2, GMCSF

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References of GM-CSF Protein: