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High Yield Taq DNA Polymerases

Taq Polymerase Enzymes, Taq Enzymes, Taq Pol, Taq

Catalog No. Product Name Size List Price (US$) Quantity
MB016-HYT-S HY Taq DNA Polymerase 1000 units 90.00
MB016-HYT-M HY Taq DNA Polymerase 5000 units 350.00
MB016-HYT-L HY Taq DNA Polymerase 10000 units 80.00
Description

MB016-HYT: High Yield Taq DNA Polymerase

The high yield Taq DNA polymerase is an optimized blend of the recombinant Taq DNA polymerase (catalog No. MB042-EUT) and a proofreading enzyme with 3′→5′ exonuclease activity. The high yield Taq DNA polymerase has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.

Unit definition: One unit of the high yield Taq DNA polymerase means incorporating 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74°C.

Concentration: 5 units/ul.

Quality Control: No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.

The PCR reagent concentrations, reaction conditions, and PCR program parameters should be determined experimentally by the investigator.

Features of the high yield Taq DNA polymerase:
* Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
* High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
* Reproducible results. No visible activity change after storage of the high yield Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
* Proofreading with 5′ exonuclease activity.

Applications of the high yield Taq DNA polymerase:
* Routine PCR using genomic, viral and plasmid templates.
* High sensitivity PCR, including difficult, low copy, or “dirty” templates.
* DNA cloning and subcloning.
* High throughput PCR.
* DNA and colony screening.
* Genotyping.
* DNA mutagenesis.
* Long PCR (up to 10 kb human genomic and 15 kb lamda DNA fragments tested).

The high yield Taq DNA polymerase is also available in 2x high yield PCR Master Mix, with blue dye (catalog No. MB040-HY2).

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.

PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.

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