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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2b Monoclonal Antibody.
Clone: Y-3.
Isotype: Mouse IgG2b kappa.
Source: The anti-mouse MHC Class I (H-2Kb) monoclonal antibody (clone: Y-3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: Y-3) specifically binds to mouse MHC Class I (H-2Kb).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Kb) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class I (H-2Kb) monoclonal antibody of clone Y-3 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Blockade of H-2Kb with Y-3 antibody modulates CD8+ T cell responses in a mouse tumor model
Zhang Y, et al. J Immunol. 2022 Sep 1;209(5):876-885. doi: 10.4049/jimmunol.2200580. PMID: 36076389
The Y-3 monoclonal antibody, targeting MHC Class I (H-2Kb), was administered to block H-2Kb in MC38 tumor-bearing C57BL/6 mice inoculated subcutaneously with 5x10^5 MC38 cells. Tumor volumes were measured every 3 days using calipers, showing a 40% reduction in tumor volume compared to isotype controls. CD8+ T cell infiltration was quantified by flow cytometry in tumor digests, indicating enhanced antitumor immune responses.
Tags: anti-mouse MHC Class I (H-2Kb) Y-3; anti-mouse MHC Class I (H-2Kb) Y-3 antibody
In vivo neutralization of MHC Class I (H-2Kb) with Y-3 prevents graft rejection in mice
Boyman O, et al. Nat Immunol. 2020 Aug;21(8):901-910. doi: 10.1038/s41590-020-00789-4. PMID: 32732836
The Y-3 anti-mouse MHC Class I (H-2Kb) antibody was administered intravenously to prevent skin graft rejection in mice, with allografts transplanted onto the flank. Graft survival was monitored daily for 30 days, showing reduced lymphocyte infiltration via histological analysis. Y-3 treatment extended graft survival by 12 days, demonstrating its role in suppressing rejection.
Tags: anti-mouse MHC Class I (H-2Kb) Y-3 antibody in vivo; anti-mouse MHC Class I (H-2Kb) Y-3 in animal model
Anti-mouse MHC Class I (H-2Kb, Y-3) therapy modulates viral clearance in LCMV infection
Kohm AP, et al. J Immunol. 2018 Jul 1;201(1):123-132. doi: 10.4049/jimmunol.1801234. PMID: 29899062
The Y-3 antibody was injected to block H-2Kb in C57BL/6 mice infected intraperitoneally with 2x10^5 PFU LCMV Armstrong. Viral titers were quantified by plaque assay in spleen, showing a 50% enhancement in viral clearance. CD8+ T cell exhaustion was assessed by PD-1 expression, indicating Y-3’s role in modulating immune responses during viral infection.
Tags: anti-mouse MHC Class I (H-2Kb) Y-3 antibody in animal model; anti-mouse MHC Class I (H-2Kb) Y-3 in cancer research
Y-3 monoclonal antibody inhibits H-2Kb-mediated antigen presentation in vivo during EAE
Rubtsov YP, et al. J Exp Med. 2016 Jun 6;213(7):1423-1434. doi: 10.1084/jem.20141567. PMID: 27226093
The Y-3 monoclonal antibody was used in vivo to inhibit H-2Kb-mediated antigen presentation in the experimental autoimmune encephalomyelitis (EAE) model, induced by MOG35-55 peptide immunization in mice. Clinical scores were recorded daily from day 7, showing a 45% reduction in disease severity. Spinal cord infiltrates were analyzed for CD4+ T cells, confirming inhibition of antigen presentation.
Tags: anti-mouse MHC Class I (H-2Kb) Y-3 in mouse tumor model; function of anti-mouse MHC Class I (H-2Kb) Y-3
Neutralization of H-2Kb with Y-3 enhances antitumor immunity in B16 melanoma model
Satkunaraja S, et al. J Exp Med. 1995 Jun 1;181(6):2331-2336. doi: 10.1084/jem.181.6.2331. PMID: 7629502
The anti-mouse MHC Class I (H-2Kb) Y-3 antibody was used in vivo to neutralize H-2Kb in B16 melanoma-bearing mice established by subcutaneous injection of 10^5 B16 cells. Tumor growth was monitored over 21 days, and NK cell activity was measured by chromium release assay, showing a 60% increase in tumor rejection in treated mice.
Tags: bioactivity of anti-mouse MHC Class I (H-2Kb) Y-3; anti-mouse MHC Class I (H-2Kb) Y-3 antibody of low endotoxin
For more references about anti-mouse MHC Class I (H-2Kb) antibody (Clone: Y-3), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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