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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2b Monoclonal Antibody.
Clone: Y-3.
Isotype: Mouse IgG2b kappa.
Source: The anti-mouse MHC Class I (H-2Kb) monoclonal antibody (clone: Y-3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: Y-3) specifically binds to mouse MHC Class I (H-2Kb).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Kb) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class I (H-2Kb) monoclonal antibody of clone Y-3 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
A Versatile Simple Capture Assay for Assessing the Structural Integrity of MHC Multimer Reagents.
Reed, B. K., et al. PLoS One. 2015 Sep 21;10(9):e0137984. doi: 10.1371/journal.pone.0137984. PMID: 26389800
Antibodies specific for particular MHC alleles, for peptide-MHC quaternary epitope determinants, or to shared epitopes such as the ?2-microglobulin (? 2M)-epitope recognized by Ab L368 were employed to assess batch to batch variation among tetramer preparations or the integrity of panels of tetramers to be used in T cell assays. ...Because human ? 2M typically is used to prepare most multimers, due to its higher affinity for both mouse and human class I heavy chain, the Ab L386 (anti- human ? 2M) was used to probe both mouse and human multimers. ...(A) Flow cytometric assessment of commercial batches of human tetramers using antibodies detecting conformational determinants expressed by folded MHC class I molecules. ...The assay can be used to compare batches of multimer preparations to provide assurance that the reagents to be used in experimental and clinical assays contain correctly assembled molecules. ...Although the assay has been designed and tested for MHC Class I multimers, a similarly compatible platform could be designed for assessing Class II multimers, or generally, any labeled reagent with conformational epitopes and available conformation sensitive antibodies.
Tags: anti-mouse MHC Class I (H-2Kb) Y-3; anti-mouse MHC Class I (H-2Kb) Y-3 antibody
Kinetics of antigen expression and epitope presentation during virus infection.
Croft, N. P., et al. PLoS Pathog. 2013 Jan;9(1):e1003129. doi: 10.1371/journal.ppat.1003129. PMID: 23382674
Recognition of these epitopes presented on MHC class I drives CD8+ T cell priming following interactions with professional antigen presenting cells (APC) and subsequently allows control of infection through killing of infected cells and secretion of cytokines. ...Further, while MHC class I antigen presentation is well understood in principle [6] and bioinformatic predictions of MHC class I binding are often highly refined [7], prediction of antigenicity and immunogenicity have remained elusive. ...MRM transitions for 8 VACV epitopes, restricted through murine Kb MHC class I molecules, were designed through spiking 200 fmol of synthetic versions of each peptide into an AB-SCIEX QTRAP 5500 mass spectrometer (Table S1 and Figure S1). ...Stabilised cell surface MHC class I complexes were visualised by flow cytometry. ...The process of MHC class I antigen presentation is complex and multi-staged. It starts with degradation of polypeptides, typically by the proteasome, followed by transport to the ER, loading onto MHC class I and finally egress to the cell surface [1].
Tags: anti-mouse MHC Class I (H-2Kb) Y-3 in mouse tumor model; function of anti-mouse MHC Class I (H-2Kb) Y-3
CD11a regulates effector CD8 T cell differentiation and central memory development in response to infection with Listeria monocytogenes.
Bose, T. O., et al. Infect Immun. 2013 Apr;81(4):1140-51. doi: 10.1128/IAI.00749-12. PMID: 23357382
After infection, we examined the localization of antigen-specific CD8 T cells by using in situ MHC class I tetramer staining and confocal microscopy (35) (Fig. 5). ...In order to enhance detection of Ova-Kb-specific CD8 T cells at early time points, we infected mice with a higher dose of an attenuated ActA-deficient strain of L. monocytogenes Ova that generates a larger response. ...After infection, a heterogeneous pool of CD8 T cell effectors is formed that can be identified based on the expression of various cell surface markers, including KLRG1 and CD127 (IL7 receptor [IL-7R]). ...At day 5 p.i., in both the WT and CD11a ?/? spleens, a readily detectable population of tetramer+ CD8 T cells were located in T cell zones (PALS) as well as in the red pulp (RP). ...Moreover, in WT and CD11a ?/? spleens, the majority of antigen-specific CD8 T cells were located in the RP, with a smaller percentage present in the B and T cell zones.
Tags: bioactivity of anti-mouse MHC Class I (H-2Kb) Y-3; anti-mouse MHC Class I (H-2Kb) Y-3 antibody of low endotoxin
For more references about Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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