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Spin Column for DNA/RNA Tiniprep

Tini DNA/RNA Purification Spin Column, Micro DNA/RNA Extraction Spin Column

Catalog No. Product Name Size List Price (US$) Quantity
MB0110S Spin Column for DNA/RNA Tiniprep 50 columns 80.00
MB0110L Spin Column for DNA/RNA Tiniprep 200 columns 240.00

MB0110-PC: Spin Column for DNA/RNA Tiniprep

The spin columns for small volume DNA elution, small amount DNA extraction and DNA concentration, small volume RNA elution, small amount RNA extraction and RNA concentration are the major component of a variety of micro DNA and RNA purification kits. The tini spin columns are designed to recover and concentrate small amount of DNA and RNA from laser capture microdissection (LCM), small number (10^1 - 10^5) of primary or cultured cells, saliva, single colony (E.coli), small tissue samples, or mouse tails. They can also be used for DNA clean-up and concentration from ChIP Assay, gel, PCR or enzyme reactions.

* The advanced design of the tini spin columns eliminates problems associated with dead volume and buffer retention, resulting in increased DNA/RNA recovery and purity, as well as minimizing the elution volume down to 5 ul. All the solutions used for tini spin columns only use 1/3 or 1/10 volume of each solution for mini spin columns.

* For clients' convenience, Syd Labs sells tini spin columns alone which are compatible to reagents in plasmid miniprep kits from most of other manufacturers, such as Qiagen and Sigma. Thus, clients do not have to waste reagents from plasmid miniprep kits.

* Low cost spin columns: $200 for 250 universal spin columns.

* Syd Labs provides the recipe of reagents for tini plasmid purification if you buy spin columns from us. Very simple to make the reagents. The shipping fee is much lower if you buy spin columns only rather than the whole DNA purification kits with heavy and bulky reagents. Save more to make your own buffer.

Applications of spin columns for small volume DNA elution, small amount DNA extraction and DNA concentration, small volume RNA elution, small amount RNA extraction and RNA concentration:

* Concentrator (down to the volume of 5 ul): Oligo (>17 bp), DNA, genomic DNA (<140 kb), RNA, and micro RNA.
* ChIP DNA clean-up and concentration (fast and efficient with high recovery in just 10 minutes).
* RNA isolation from LCM (laser capture microdissection) samples.
* Preparation of nanogram to milligram amount of DNA or RNA from saliva, plasma, serum, whole blood, tissue samples (such as mouse tails), virus, bacterial, plants, and others sources.
* DNA and RNA nano-prep (10 ng to 20 ug) directly from single colony (>2 mm diameter) on plate after E.coli transformation without growing 2 ml overnight culture.
* DNA and RNA gel extraction.
* Cleaning up and concentrating DNA and RNA from PCR products, enzymatic reactions, labeling, and sequencing reactions.
* Micro RNA (small RNA) preparation and clean up.

Case study: plasmid tiniprep from a single E.coli colony

1) Pick a signle colony (about 2 mm) from a plate;
2) Resuspend the colony in 15 ul solution I;
3) Add 15 ul solution II and then 22.5 ul solution III;
4) Mix gently by tapping the tube with finger and then spin with 12,000 rpm for 5 min;
5) Load the supernatant to a tini spin column;
6) Spin and discard flow-through and then wash with 100 ul washing buffer;
7) Elute the DNA with 5-10 ul TE or H2O.

Other DNA and RNA Purification Spin Columns:
Spin column for plasmid miniprep
Spin column for plasmid maxiprep
Spin column for PCR product cleanup
Spin column for gel extraction

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR PreMix with dye (green, red, blue).
* 2x rapid PCR Master Mix with a green dye.
* 2x long PCR Master Mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT-PCR master mixes
* 2x qPCR master mixes
* RT-qPCR master mixes

PCR components and related products:
* dNTP mix, 10 mM each.
* dNTP mix, 25 mM each.
* Proteinase K powder .
* Proteinase K solution.
* Reverse Transcriptases.
* First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* PCR enhancer.

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