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DNA Assembly Cloning Kits

Non-Ligase Dependent DNA Cloning, Homologous Recombination DNA Assembly Cloning Kit

Catalog No. Product Name Size List Price (US$) Quantity
MB125 Universal DNA Assembly Cloning Kit 25 reactions 120.00
MB125 Universal DNA Assembly Cloning Kit 50 reactions 200.00
MB126 Simple DNA Assembly Cloning Kit 25 reactions 100.00
MB126 Simple DNA Assembly Cloning Kit 50 reactions 160.00
Description

MB125: Universal DNA Assembly Cloning Kit

Our DNA assembly cloning kits are based on a simple, fast and efficient DNA seamless cloning technology, and can be used to clone any insert into any position of any vector. The unique non-ligase dependent DNA cloning system greatly reduces the background resulting from vector self-ligation so that the positive rate can reach more than 95%. The highly optimized reaction buffer and the enhanced recombinase significantly improve the recombination efficiency of cloning and the tolerance to impurities, making it possible for linearized vectors and inserts to be directly used for homologous recombination cloning without DNA purification, greatly simplifying the process of DNA cloning.

The universal DNA assambly cloning kit can be used for cloning up to 5 inserts to a single vector in one step.

Features of the Universal DNA Assembly Cloning Kit:
* Simple and convenient: mix one or multiple linearized inserts and the vector in one tube, and incubate them for 5 to 15 minutes at 50°C. One tube, one step.
* Fast and efficient with high positive rate and low background.
* No restriction enzymatic site(s) is needed.
* Multiple linearized inserts: 1 to 5.
* Insert size: 50 bp to 10 kb.

Applications of the Universal DNA Assembly Cloning Kit:
* Fast cloning
* High-throughput cloning
* Seamless assembly
* Site-directed mutagenesis.

High-fidelity DNA polymerases and high-fidelity PCR master mixes are available.

Please contact us if you need protocol, datasheet, or SDS of our universal DNA assambly cloning kit.

MB126: Simple DNA Assembly Cloning Kit

The simple DNA assambly cloning kit can be used for cloning 1 insert to a single vector in one step.

Features of the Simple DNA Assembly Cloning Kit:
* Simple and convenient: mix one linearized insert and the vector in one tube, and incubate them for 5 minutes at 50°C. One tube, one step.
* Fast and efficient with >95% positive rate.
* No restriction enzymatic site(s) is needed.
* Multiple linearized inserts: 1.
* Insert size: 50 bp to 10 kb.

Applications of the Simple DNA Assembly Cloning Kit:
* Fast cloning
* High-throughput cloning
* Seamless assembly
* Site-directed mutagenesis.

Please contact us if you need protocol, datasheet, or SDS of our simple DNA assambly cloning kit.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR PreMix with dye (green, red, blue).
* 2x rapid PCR Master Mix with a green dye.
* 2x long PCR Master Mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT-PCR master mixes
* 2x qPCR master mixes
* RT-qPCR master mixes

PCR components and related products:
* dNTP mix, 10 mM each.
* dNTP mix, 25 mM each.
* Proteinase K powder.
* Proteinase K solution.
* Reverse Transcriptases.
* First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* PCR enhancer.

Questions and Answers:

Question: Do you provide free sample of your hot start Taq DNA polymerase?
Answer: Sorry that we do not provide free sample. You can place an order and we will hold the invoice until you tell us that you like our product. You do not need to pay us if you do not like the product. It is an alternative to free sample. 

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