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Home > Molecular Biology > Cloning and Expression > First-Strand cDNA Synthesis
Kits and components for first-strand cDNA synthesis.
    3 First-Strand cDNA Synthesis Products
  • MB127-5G: 5G RT Supermix for qPCR with gDNA Elimination Function

    * The 5G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
    * Simple and fast operation: one-step genome clearance and reverse transcription.
    * Higher reverse transcription efficiency and higher cDNA yields from all regions of RNA transcripts: based on the more efficient 5G Reverse Transcriptase.
    * Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs.
    * Excellent cDNA stability: complete deactivation of thermal DNase and long-term storage of cDNA.
     

    MB127-4G: 4G RT Supermix for qPCR with gDNA Elimination Function

    * The 4G RT supermix for qPCR with gDNA elimination function is suitable for two-step RT-qPCR detection, and compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.
    * Based on the highly efficient 4G Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA.
    * Quick and complete removal of genome contamination by the 4 × gDNA Removing Buffer: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns.
    * Different primers are selected for different downstream applications.

  • MB036-5G: 5G Reverse Transcriptase

    * Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
    * Super impurity tolerance: tolerant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
    * Higher reverse transcription efficiency than 4G reverse transcriptase.

     

    MB036-4G: 4G Reverse Transcriptase

    * Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
    * Super impurity resistance: resistant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
    * Long-fragment cDNA amplification (as long as 20 kb).

     

    MB036-MM: M-MLV(H-) Reverse Transcriptasee

    * Point mutations eliminate RNase H activity to obtain long cDNA products ≤5 kb.
    * Stable and reliable reverse transcription performance for RNA templates above 100 ng.
    * For 5’-RACE reaction and cDNA library construction.

  • MB037-5G: 5G First Strand cDNA Synthesis Kit with gDNA Elimination Function

    * Higher reverse transcription efficiency and higher cDNA yields from all regions of RNA transcripts: based on the more efficient 5G Reverse Transcriptase.
    * Flexible choice of primers: Different types of reverse transcription primers can be used flexibly for different experimental designs.
    * Quick and complete removal of genome contamination by the 5× gDNA Removing Buffer: to ensure more reliable follow-up results, and simplify the design of qPCR primers, without the need to design primers across introns.

     

    MB037-4G: 4G First Strand cDNA Synthesis Kit with gDNA Elimination Function

    * Based on the highly efficient 4G Reverse Transcriptase, the super temperature tolerance ensures that the complex secondary structure of RNA can be opened under high temperature conditions to obtain longer cDNA.
    * A wide range of template starting amount: from 1 pg - 5 μg total RNA.
    * Long fragment amplification: up to 15 kb or more.
    * Anchored Oligo (dT)23 VN primer is designed for binding site anchoring with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis.
    * Different primers are selected for different downstream applications. The synthesized one-strand cDNA is widely used in molecular cloning, hybridization, PCR amplification and qPCR reaction, and so on.

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