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DYKDDDDK-Tag Antibody

Binds to the same epitope as Sigma 'Anti-FLAG' M2 Antibody

Catalog No. Product Name Size List Price (US$) Quantity
PA000274.h1 In vivo Grade Recombinant DYKDDDDK-Tag Monoclonal Antibody (M2, Human IgG1 Kappa) 1 mg 490.00
PA000274.h1 In vivo Grade Recombinant DYKDDDDK-Tag Monoclonal Antibody (M2, Human IgG1 Kappa) 5 mg 1000.00
PA000274.h1 In vivo Grade Recombinant DYKDDDDK-Tag Monoclonal Antibody (M2, Human IgG1 Kappa) 20 mg 2000.00
Description

PA000274.h1: In vivo Grade Recombinant DYKDDDDK-Tag Monoclonal Antibody (M2, Human IgG1 Kappa)

The in vivo grade recombinant DYKDDDDK-Tag monoclonal antibody (M2, human IgG1 kappa) is a chimeric mouse/human monoclonal antibody produced in mammalian cells. It consists of mouse variable regions and human IgG1 kappa constant regions. 
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form of Antibody: 1 mg/ml in 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Clone: M2.
Isotype: human IgG1.

Shipping: The in vivo grade recombinant DYKDDDDK-Tag monoclonal antibody (M2, human IgG1 kappa) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Other DYKDDDDK Antibodies:

DYKDDDDK-tag Monoclonal Antibody
DYKDDDDK-tag Monoclonal Antibody (Magnetic Microbead-Conjugated)
DYKDDDDK-tag Monoclonal Antibody (Agarose-Conjugated)

DYKDDDDK Peptide:
DYKDDDDK Peptide

Other Tag Antibodies:
His-tag Monoclonal Antibody
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
HA-tag Monoclonal Antibody
Myc-tag Monoclonal Antibody
V5-tag Monoclonal Antibody

Loading Control Antibodies:
GAPDH Monoclonal Antibody
beta-Actin Monoclonal Antibody
beta-Tubulin Monoclonal Antibody

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