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Home > Antibodies > Primary Antibodies for Epitope Tags  > DYKDDDDK-Tag Antibody

DYKDDDDK-Tag Antibody

the same epitope detected by the Sigma-Aldrich 'Anti-FLAG' M2 Antibody

Catalog No. Product Name Size List Price (US$) Quantity
PA000274.m1 Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa 50 ug 100.00
PA000274.m1 Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa 100 ug 150.00
PA000274.m1 Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa 200 ug 275.00
PA000274.m1 Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa 1 mg 490.00
PA000274.m1 Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa 5 mg 1300.00
PA000274.mf Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1) 1 mg 900.00
PA000274.scFv Recombinant Anti-DYKDDDDK-Tag scFv (clone L1.1), His Tag 1 mg 900.00
PA000274.h1 Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1),Human lgG1 Kappa 1 mg 490.00
PA000274.h1 Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1),Human lgG1 Kappa 5 mg 1500.00
PA000274.rt Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1), Rabbit IgG 1 mg 490.00
Description

PA000274.m1: Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

The recombinant anti-DYKDDDDK-Tag mouse IgG1 monoclonal antibody (clone L1.1) is produced in mammalian cells. It consists of mouse variable regions and mouse IgG1 kappa constant regions. 
Clone: L1.1.
Isotype: mouse IgG1 kappa.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody recognizes the same epitope as the 'Anti-FLAG' M2 antibody detects.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein G.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.

PA000274.h1: In vivo Grade Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody, Human IgG1 (clone L1.1)

The in vivo grade recombinant anti-DYKDDDDK-Tag human IgG1 monoclonal antibody (clone L1.1) is a chimeric mouse/human monoclonal antibody produced in mammalian cells. It consists of mouse variable regions and human IgG1 kappa constant regions. 
Clone: L1.1.
Isotype: human IgG1 kappa.
Specificity/Sensitivity: detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. The antibody recognizes the same epitope as the 'Anti-FLAG' M2 antibody detects.
Applications: ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype controls. 
Reactivity: Human, Mouse, Rat, Others.
Purification: The DYKDDDDK-tag antibody was affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A.
Form of Antibody: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant anti-DYKDDDDK-Tag monoclonal antibody (clone L1.1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap.
1 month from date of receipt, 2 to 8°C as supplied.
12 months from date of receipt, -20°C to -70°C as supplied.

References of Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1)

Weiwei Peng, et al. J Proteome Res. 2021 Jul 2;20(7):3559-3566. doi: 10.1021/acs.jproteome.1c00169. PMID: 34121409; PMCID: PMC8256418
“Antibody sequence information is crucial to understanding the structural basis for antigen binding and enables the use of antibodies as therapeutics and research tools. Here, we demonstrate a method for direct de novo sequencing of monoclonal IgG from the purified antibody products. The method uses a panel of multiple complementary proteases to generate suitable peptides for de novo sequencing by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in a bottom-up fashion.”
(Tags: De Novo Sequencing; Monoclonal Antibodies)
Katsuhiko Matsumoto, et al. PLoS Biol. 2025 Jan 23;23(1):e3002707. doi: 10.1371/journal.pbio.3002707. PMID: 39847587; PMCID: PMC11756784
“Antibodies are extensively used in biomedical research, clinical fields, and disease treatment. However, to enhance the reproducibility and reliability of antibody-based experiments, it is crucial to have a detailed understanding of the antibody's target specificity and epitope. In this study, we developed a high-throughput and precise epitope analysis method, DECODE (Decoding Epitope Composition by Optimized-mRNA-display, Data analysis, and Expression sequencing).”
(Tags: Epitope Mapping; High Throughput Analysis)
Xin-Yu Guo, et al. PLoS One. 2021 May 5;16(5):e0250805. doi: 10.1371/journal.pone.0250805. PMID: 33951064; PMCID: PMC8099120
“A FLAG tag consisting of DYKDDDDK is an epitope tag that is frequently and widely used to detect recombinant proteins of interest. In this study, we performed a CRISPR-based genetic screening to identify factors involved in the detection of a FLAG-tagged misfolded model protein at the cell surface. In the screening, SLC35B2, which encodes 3'-phosphoadenosine-5'-phosphosulfate transporter 1, was identified as a necessary component.”
(Tags: FLAG Tag; Antibody Recognition)
Takushi Miyoshi, et al. Cell Rep. 2021 Feb 2;34(5):108708. doi: 10.1016/j.celrep.2021.108708. PMID: 33535030; PMCID: PMC7904085
“Fast-dissociating, specific antibodies are single-molecule imaging probes that transiently interact with their targets and are used in biological applications including image reconstruction by integrating exchangeable single-molecule localization (IRIS), a multiplexable super-resolution microscopy technique. Here, we introduce a semi-automated screen based on single-molecule total internal reflection fluorescence (TIRF) microscopy of antibody-antigen binding, which allows for identification of fast-dissociating monoclonal antibodies directly from thousands of hybridoma cultures. We develop monoclonal antibodies against three epitope tags (FLAG-tag, S-tag, and V5-tag) and two F-actin crosslinking proteins (plastin and espin).”
(Tags: Single Molecule Microscopy; Antibody Screening)
Sebastiaan C de Graaf, et al. MAbs. 2022 Jan-Dec;14(1):2079449. doi: 10.1080/19420862.2022.2079449. PMID: 35699511; PMCID: PMC9225641
“A key step in therapeutic and endogenous humoral antibody characterization is identifying the amino acid sequence. So far, this task has been mainly tackled through sequencing of B-cell receptor (BCR) repertoires at the nucleotide level. Mass spectrometry (MS) has emerged as an alternative tool for obtaining sequence information directly at the - most relevant - protein level.”
(Tags: Endogenous Antibodies; Mass Spectrometry)

Other DYKDDDDK Antibodies:
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
Recombinant Anti-DYKDDDDK-Tag Mouse Fab Fragment (Clone L1.1)
Recombinant Anti-DYKDDDDK-Tag scFv His Tag (clone L1.1)
Recombinant Anti-DYKDDDDK-Tag Poly Mouse Fab (clone L1.1)
Recombinant Anti-DYKDDDDK-Tag Poly scFv (clone L1.1)
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1),Human lgG1 Kappa
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1), Rabbit IgG

Biotinylated DYKDDDDK Antibodies:
Biotinylated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1), Human IgG1 Fc Silent Kappa
Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1),Human IgG1 Fc Silent Kappa-AviTag
Biotinylated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

DYKDDDDK Antibodies Conjugated with Agarose or Magnetic Microbead:
DYKDDDDK-tag Monoclonal Antibody (Magnetic Microbead-Conjugated)
DYKDDDDK-tag Monoclonal Antibody (Agarose-Conjugated)

DYKDDDDK Antibodies for Flow Cytometry:
HRP Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
FITC Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
IFluor 488 Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
APC Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa
PE Conjugated Recombinant Anti-DYKDDDDK-Tag Monoclonal Antibody (clone L1.1), Mouse IgG1 Kappa

DYKDDDDK Peptide:
DYKDDDDK Peptide

Other Tag Antibodies:
DYKDDDDK-Tag Monoclonal Antibody (Clone L1.1)
Myc-Tag Monoclonal Antibody (Clone 9E10)
HA-Tag Monoclonal Antibody (Clone 12CA5)
V5-tag Monoclonal Antibody (Clone SV5-Pk1)
Strep-Tag Monoclonal Antibody (Clone C23.21)
His-Tag Monoclonal Antibody (Clone 3D5)
His-tag Monoclonal Antibody (Clone HIS.H8)
GFP-tag Monoclonal Antibody
RFP-tag Monoclonal Antibody
His-Tag Monoclonal Antibody (Clone 26D11)

Loading Control Antibodies:
GAPDH Monoclonal Antibody
beta-Actin Monoclonal Antibody
beta-Tubulin Monoclonal Antibody

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