BP4200: Recombinant Dr. Nuclease (Benzonase Nuclease Alternative)
Source: E. coli-derived.
Specific activity: ≥1.1 x 10^6 U/mg. One unit of Dr. Nuclease is defined as the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.
Purity: ≥95% by SDS-PAGE and ≥99% by SEC-HPLC. It does not contain any antimicrobial preservatives or protein stabilizers except glycerol (of synthetic origin).BP4200-GMP: Recombinant Dr. Nuclease (Benzonase Nuclease Alternative), GMP Grade
Source: E. coli-derived.
Predicted molecular mass: ~28 kDa.
Specific activity: ≥1.1 x 10^6 U/mg. One unit of Dr. Nuclease is defined as the amount of enzyme required to produce a change in absorbance at 260 nm of 1.0 in the time of 30 minutes, under optimum conditions with excess substrate.
Purity: ≥95% by SDS-PAGE and ≥99% by SEC-HPLC. It does not contain any antimicrobial preservatives or protein stabilizers except glycerol (of synthetic origin).
Formulation: 0.2 μM filtered solution (250 units/ul) of 20 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 2 mM NaCl, 50% Glycerol.
Endotoxin level: ≤0.01 EU/1000 units following 2020 ChP 1143 USP <85>.
Protease activity: No detectable protease activity using casein as substrate.
Residual host cell protein: ≤10.0 ppm by ELISA.
Mycoplasma: negative by qPCR method.
Bioburden: negative following 2020 ChP 1105 USP <61>.
Residual heavy metal: ≤10.0 ppm following 2020 ChP 0821 USP <32>.MB112: Proteinase K Solution, PCR Grade
Proteinase K is commonly used for protein digestion, DNA extraction, RNA purification, direct PCR, and genotyping. The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant enzyme production has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
High purity: Each lot of the recombinant proteinase K solution (molecular biology grade, PCR grade, NGS grade, chip grade, and RNA grade) was tested to ensure the absence of DNases and RNases, and free of DNA.
The cheapest proteinase K solution in the market.
Specific activity: ≥ 780 units/ml.MB112: Proteinase K Solution, PCR Grade
Proteinase K is commonly used for protein digestion, DNA extraction, RNA purification, direct PCR, and genotyping. The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant enzyme production has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
High purity: Each lot of the recombinant proteinase K solution (molecular biology grade, PCR grade, NGS grade, chip grade, and RNA grade) was tested to ensure the absence of DNases and RNases, and free of DNA.
The cheapest proteinase K solution in the market.
Specific activity: ≥ 780 units/ml.MB111: Proteinase K Powder
The recombinant proteinase K enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale manufacturing of the recombinant enzyme has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of the recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
High purity: >99.5% by reducing SDS-PAGE. Each lot of the proteinase K powder was tested to ensure the absence of Nucleases and DNA. It can be used to make molecular biology grade proteinase K solution, PCR grade proteinase K solution, NGS grade proteinase K solution, CHIP grade proteinase K solution, and so on.
Specific activity: ≥ 34 units/mg of protein.
The lowest proteinase K price in the market.BP004089-GD29: Recombinant PNGase F Enzyme (glycerol free)
Source: E.coli-derived.
Unit Definition: One unit of the PNGase F enzyme catalyzes the release of >95% N-linked glycan from 10 ug of denatured RNase B in 10 ul at pH 7.5 in 1 hour at 37°C. One Syd Labs unit of PNGase F activity is equal to 0.015 IUB milliunit.
Purity: > 95% by SDS-PAGE and SEC-HPLC.BP000034-GD8: Recombinant Bovine Enterokinase, Light Chain
Source: Yeast-derived.
Unit Definition: 1 IU of Enterokinase (EK) will cleave 50 ug of test substrate to 95% completion in 16 hours or less at 25°C.
Unit Assay Condition: 20 mM Tris-HCl (pH 8.0 @ 25°C), 50 mM NaCl, 2 mM CaCl2, 25 ug of an MBP fusion protein test substrate and enzyme. Incubate at 23°C.BP000031-GD5: Recombinant Heparinase I from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >100 IU/mg. One international unit (IU) of recombinant Heparinase I is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0.
Purity >95% by SDS-PAGE.BP000032-GD6: Recombinant Heparinase II from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >6 IU/mg. One international unit (IU) of recombinant Heparinase II is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.BP000033-GD7: Recombinant Heparinase III from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >50 IU/mg. One international unit (IU) of recombinant Heparinase III is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.BP4114: Recombinant endotoxin-free TEV protease
Source: E.coli-derived.
Purity: >95% by SDS-PAGE under reducing conditions.
Activity: Recombinant endotoxin-free TEV protease cleaves after Gln in the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln.
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.