Anti-Human HLA-DR Monoclonal Antibodies

PA007453: In vivo Grade Recombinant Anti-Human HLA-DR Monoclonal Antibody, Mouse IgG2a Kappa (Clone: L243)

The in vivo grade recombinant anti-human HLA-DR Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells. 
Clone: L243.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: L243) specifically binds to human HLA-DR.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human HLA-DR protein. 
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The in vivo grade recombinant anti-human HLA-DR antibodies (clone of L243) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Human HLA-DR Monoclonal Antibody:

Citrullinated Vimentin Presented on MHC-II in Tumor Cells Is a Target for CD4+ T-Cell-Mediated Antitumor Immunity
Brentville, V. A., et al. Cancer Res. 2016 Feb 1;76(3):548-60. doi: 10.1158/0008-5472.CAN-15-1085. PMID: 26719533
To construct the plasmid pVitro 2 Chimeric HLA-DR401, cDNA was generated from mRNA isolated from the splenocytes of transgenic HLA-DR4 mice. ...Following sequence confirmation full-length chimeric α chain comprising murine H2-Ea with the human HLA-DRA α 1 domain was ligated into the FspI/EcoRI mcs2 of the vector pVITRO2-hygro-mcs (Invivogen). ...To generate the IFNγ-inducible plasmid pDCGAS chimeric HLA-DR401, the chimeric α and β chains, were cloned into the pDCOrig vector described elsewhere (24) in replacement of the heavy and light chain. ...The CMV promoter within each cassette was excised and replaced with the IFNγ-inducible promoter driving expression of the HLA-DR401 chains within the pDCOrig vector backbone. ...For MHC blocking studies 20 μg/mL of the HLA-DR (clone L243)-specific antibody was added to Elispot assays.
Tags: anti-human HLA-DR L243; anti-human HLA-DR L243 mAb

The Possible Mechanism of Idiosyncratic Lapatinib-Induced Liver Injury in Patients Carrying Human Leukocyte Antigen-DRB1*07:01
Hirasawa, M., et al. PLoS One. 2015 Jun 22;10(6):e0130928. doi: 10.1371/journal.pone.0130928. PMID: 26098642
Although the expressions of these two alleles are highly correlated, comparisons of HLA-DR and HLA-DQ heterodimer protein pairing suggested the HLA-DRB1*07:01 allele provided the simplest causal association with lapatinib-induced ALT elevation. ...In this study, we evaluated the effect of lapatinib on the binding of the known ligand peptides to HLA-DRB1*07:01 and DRB1*15:01 in vitro. ...Also we performed a series of in silico molecular modeling for HLA-DRB1*07:01 and two other HLA-DRs to further assess the possible role of lapatinib on HLA-peptide binding, which could lead to the mechanism of immune-mediated lapatinib-induced liver injury. ...The finding motivated us to predict the possible interaction mode of lapatinib with HLA-DRs. ...In summary, our in vitro experiments revealed that lapatinib enhances binding of the ligand peptide to HLA-DRB1*07:01 preferentially.
Tags: anti-human HLA-DR L243 antibody in vivo; anti-human HLA-DR L243 antibody in animal model

Preserved dendritic cell HLA-DR expression and reduced regulatory T cell activation in asymptomatic Plasmodium falciparum and P. vivax infection
Kho, S., et al. Infect Immun. 2015 Aug;83(8):3224-32. doi: 10.1128/IAI.00226-15. PMID: 26034211
Peripheral blood total DC were identified by flow cytometry as lineage marker-negative HLA-DR+ cells. ...The activation/maturation of circulating blood DC was examined by measuring the median fluorescence intensity of HLA-DR expression. ...In a limited number of samples, additional staining was possible and HLA-DR expression could be examined on two myeloid DC (mDC) subsets, CD1c+ (blood dendritic cell antigen 1 [BDCA-1] positive) mDC (CD1c+ mDC) and CD141+ (BDCA-3 positive) mDC (CD141+ mDC). ...In both mDC subsets, HLA-DR was preserved during asymptomatic infection, but in 3 adults with uncomplicated malaria, HLA-DR expression appeared reduced. ...Total DC HLA-DR expression was inversely correlated with parasitemia in adults with asymptomatic P. falciparum infection (rs = −0.54, P = 0.02, n = 18) and positively correlated in children with asymptomatic P. vivax infection (rs = 0.80, P = 0.01, n = 9).
Tags: anti-human HLA-DR L243 in cancer research; anti-human HLA-DR L243 antibody in cancer research

Differential incorporation of CD45, CD80 (B7-1), CD86 (B7-2), and major histocompatibility complex class I and II molecules into human immunodeficiency virus type 1 virions and microvesicles: implications for viral pathogenesis and immune regulation
Esser, M. T., et al. J Virol. 2001 Jul;75(13):6173-82. doi: 10.1128/JVI.75.13.6173-6182.2001. PMID: 11390619
The HIV virion envelope is enriched for HLA-DR but not DP or DQ (2, 6, 18, 45), and virion-associated MHC-II can bind and present the superantigen Staphylococcus enterotoxin B to resting T cells, resulting in T-cell activation. ...Immunofluorescent staining of PBMC and MDMs (3 × 105 per condition) was performed at 4°C for 30 min by using isotype immunoglobulin G1 (IgG1) (X40), IgG2a (X39), and V4 (non-gp120-interacting domain on CD4) and HLA-DR (L243) MAbs from Becton Dickinson Immunocytometry Systems (San Jose, Calif.). ...The HLA-DR genotype of the T1, T2, TBLCL-CD4, and H9 cell lines; the phenotype of the virion envelope; and whether the HIV-1NL4-3-AT2 virions triggered cell death are summarized in Table. ...Future studies will determine whether the HLA-DR phenotype of the virus or the responder PBMC affects HIV-1-triggered apoptosis. ...We thank Mike Grimes and Bill Bohn for the production and characterization of sucrose-banded, purified HIV-1NL4-3, Antonio Valentin and Jim Turpin for the generous gifts of selected HIV-1 isolates, and Darlene Marti and Mary Carrington for HLA-DR genotyping of the PBMC donor and cell lines.
Tags: function of anti-human HLA-DR L243 antibody; bioactivity of anti-human HLA-DR L243 mAb

For more references about Anti-Human HLA-DR Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2a isotype control antibody

Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry