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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human HLA-DR Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells.
Clone: L243.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: L243) specifically binds to human HLA-DR.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human HLA-DR protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human HLA-DR antibodies (clone of L243) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Nonclinical immunogenicity risk assessment for knobs-into-holes bispecific IgG1 antibodies
Tsai, W-T. K., et al. (2024). mAbs. 2024 Dec;16(1):2362789. doi: 10.1080/19420862.2024.2362789. PMID: 38845069
The L243 antibody (anti-human HLA-DR) was used in a mixed immunoprecipitation strategy with WR18 (pan-HLA-II) and SPV-L3 (HLA-DQ-specific) to enhance compound-specific peptide detection for immunogenicity risk assessment of bispecific IgG1 antibodies. The approach showed that knobs-into-holes mutations and Fab mutations for bispecific assembly posed low immunogenicity risk compared to benchmark antibodies like Avastin and Herceptin.
Tags: anti-human HLA-DR L243; anti-human HLA-DR L243 mAb
Disulfiram Inhibits Opsonin-Independent Phagocytosis of In Vivo Activated Human Macrophages and Dendritic Cells
Schulze, F. S., et al. (2024). Cells. 2024 Mar 18;13(6):521. doi: 10.3390/cells13060521. PMID: 38534379
The L243 antibody (anti-human HLA-DR) was used to stain macrophages for surface phenotypic marker assessment via flow cytometry, alongside antibodies for CD45, CD14, CD163, and CD38. Cells were stained for 30 minutes on ice, washed, and analyzed to evaluate the effects of disulfiram (50 nmol·L⁻¹) on phagocytosis in cultured M1 and M2a polarized macrophages and dendritic cells.
Tags: anti-human HLA-DR L243 antibody in vivo; anti-human HLA-DR L243 antibody in animal model
HLA Class II Antibody Activation of Endothelial Cells Promotes Th17 and Disrupts Regulatory T Lymphocyte Expansion
Lion, J., et al. (2016). Am J Transplant. 2016 May;16(5):1408-20. doi: 10.1111/ajt.13644. PMID: 26614587
The L243 antibody (APC-conjugated, anti-human HLA-DR) was used to phenotype endothelial cells (ECs) via flow cytometry, confirming HLA-DR expression. Its F(ab')₂ fragment activated ECs, inducing Akt, ERK, and MEK phosphorylation and IL-6 production in cocultures with PBMCs, promoting Th17 polarization and reducing functional Treg expansion in an Akt-dependent manner.
Tags: anti-human HLA-DR L243 in cancer research; anti-human HLA-DR L243 antibody in cancer research
Evaluation of anti-human leukocyte antigen-DR monoclonal antibody therapy in spontaneous canine lymphoma
Stein, R., et al. (2011). Leuk Lymphoma. 2011 Feb;52(2):273-84. doi: 10.3109/10428194.2010.535182. PMID: 21133722
The L243 antibody (anti-human HLA-DR) induced apoptosis in canine lymphoma cells in vitro and showed transient disease stabilization in dogs with advanced-stage lymphoma. The humanized IgG4 version, hL243γ4P (IMMU-114), exhibited similar binding and pharmacokinetic profiles in normal dogs, supporting its potential for both veterinary and human lymphoma therapy.
Tags: function of anti-human HLA-DR L243 antibody; bioactivity of anti-human HLA-DR L243 mAb
Humanization and characterization of the anti-HLA-DR antibody 1D10
Kostelny, S. A., et al. (2001). Int J Cancer. 2001 Aug 15;93(4):556-65. doi: 10.1002/ijc.1366. PMID: 11477560
The 1D10 antibody, which recognizes some HLA-DR beta chains, was humanized and evaluated for anti-tumor activity against B-cell malignancies. Compared to L243 (anti-human HLA-DR), humanized 1D10 retained direct antilymphoma activity without effector functions and enhanced rituximab’s potency, warranting further evaluation as an immunotherapeutic agent.
Tags: anti-human HLA-DR antibody L243; clone L243 of anti-human HLA-DR mAb
For more references about anti-human HLA-DR antibody (L243), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2a isotype control antibody
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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