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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 9.3F10.
Isotype: Mouse IgG2a Kappa.
Source: The anti-human HLA class II DR/DQ monoclonal antibody (clone: 9.3F10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 9.3F10 antibody specifically binds to human HLA class II DR/DQ.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Recombinant Human IgG1 Monoclonal Antibody with Fc silent mutation.
Clone: 9.3F10.
Isotype: Human IgG1 kappa.
Source: The anti-human HLA class II DR/DQ monoclonal antibody (clone: 9.3F10) was produced in mammalian cells.
Conjugation: Non-conjugated.
Specificity/Sensitivity: The recombinant 9.3F10 antibody specifically binds to human HLA class II DR/DQ.
Applications: Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form of Antibody: 0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Purity: >95% by SDS-PAGE under reducing conditions.
Shipping: The 9.3F10 antibody are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
IgG-Based Bispecific Anti-CD95 Antibodies for the Treatment of B Cell-Derived Malignancies and Autoimmune Diseases.
Hörner, S., et al. (2022). Cancers (Basel). 2022 Aug 16;14(16):3941. doi: 10.3390/cancers14163941. PMID: 36010934
The study utilized codon-optimized variable domains of humanized Apo-1, 2H7, 4G7 (anti-human CD19), and MOPC-21 antibodies for transfection into CHO cells. Bispecific antibodies combined the CD95 agonist Apo-1 with either anti-CD20 (clone 2H7) or anti-CD19 (clone 4G7) in F(ab)2 and single-chain formats. The CD19 binding affinity showed an EC50 of approximately 7.5 nM, despite 4G7’s higher affinity compared to 2H7. Flow cytometry revealed CD20 expression was 3–10-fold higher than CD19, with Raji cells expressing over 20,000 CD19 and 190,000 CD20 molecules per cell.
Tags: 4G7 antibody for flow cytometry; 4G7 monoclonal antibody
Reduction of Minimal Residual Disease in Pediatric B-lineage Acute Lymphoblastic Leukemia by an Fc-optimized CD19 Antibody.
Seidel, U. J., et al. (2016). Mol Ther. 2016 Sep 6;24(9):1634-43. doi: 10.1016/j.ymthe.2016.06.008. PMID: 27380762
The Fc-optimized CD19 antibody 4G7SDIE, a humanized version of the murine 4G7 antibody (anti-human CD19) with S239D and I332E modifications, was tested in B-lineage ALL blasts from 18 patients and 5 4G7SDIE-treated patients. The antibody was detected via indirect immunofluorescence staining and flow cytometry. 4G7SDIE enhanced antibody-dependent cellular cytotoxicity (ADCC) against leukemic blasts, showing promise for minimal residual disease (MRD) treatment due to favorable target-to-effector cell ratios.
Tags: anti-human CD19 4G7 mAb for flow cytometry; anti-human CD19 4G7 of low endotoxin
Heterodimeric bispecific antibody-derivatives against CD19 and CD16 induce effective antibody-dependent cellular cytotoxicity against B-lymphoid tumor cells.
Kellner, C., et al. (2011). Cancer Lett. 2011 Jul 28;306(1):88-95. doi: 10.1016/j.canlet.2011.02.023. PMID: 21339041
The study utilized the 4G7 hybridoma (anti-human CD19; IgG1) and 3G8 (CD16; IgG1) to create Fab–scFv fusion proteins combining CD19-directed Fab fragments with CD19 and CD16 scFvs. CD19, a 95 kDa transmembrane glycoprotein, is a promising target for B-cell malignancy treatments due to its favorable properties. The bispecific antibodies demonstrated effective antibody-dependent cellular cytotoxicity (ADCC) against B-lymphoid tumor cells, highlighting CD19’s potential in immunotherapy.
Tags: anti-CD19 (4G7) monoclonal antibody; human CD19 antibody (clone 4G7)
Potent In vitro and In vivo Activity of an Fc-Engineered Anti-CD19 Monoclonal Antibody against Lymphoma and Leukemia.
Horton, H. M., et al. (2008). Cancer Res. 2008 Oct 1;68(19):8049-57. doi: 10.1158/0008-5472.CAN-08-1265. PMID: 18829563
The study developed XmAb5574, a humanized and affinity-matured version of the mouse anti-CD19 antibody 4G7 with S239D/I332E Fc modifications. XmAb5574 and other anti-CD19 antibodies showed approximately 2-fold higher binding affinity than 4G7. In vitro, target cells opsonized with antibodies were mixed with NK cells, and LDH release was measured. In vivo, fluorescence-activated cell sorting confirmed CD19 expression and tumor cell percentages, demonstrating potent activity against lymphoma and leukemia.
Tags: anti-human CD19 4G7 in animal model; anti-human CD19 4G7 mAb for flow cytometry
For more references about anti-human HLA class II DR/DQantibody (9.3F10), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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