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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 14-4-4S.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse mouse/rat MHC Class II (I-Ek/RT1-D) monoclonal antibody (clone: 14-4-4S) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 14-4-4S) specifically binds to mouse/rat MHC Class II (I-Ek/RT1-D).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse/rat MHC Class II (I-Ek/RT1-D) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse/rat MHC Class II (I-Ek/RT1-D) monoclonal antibody of clone 14-4-4S is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Accelerated turnover of MHC class II molecules in nonobese diabetic mice is developmentally and environmentally regulated in vivo and dispensable for autoimmunity
Unanue, E. R., et al. J Immunol. 2013 Jun 15;190(12):5969-77. doi: 10.4049/jimmunol.1203415. PMID: 23677470
(A) MHCII molecules were immunoprecipitated with 14-4-4S, IgG2a anti-H2-Ed (left); MK-D6, IgG2a anti-H2-Ad (center); and OX-6, IgG1 anti-H2-Ag7 (right) and either protein A- or G-sepharose from BALB/c or NOD splenic B cells (2-3 ×10^7), resolved by SDS-PAGE under nonreducing conditions, and visualized by silver staining. Fractional MHCII protein synthesis was quantified in B cells isolated after 4 hours of 2H2O labeling in vivo. Data are shown for different peptides (identified by the first three amino acids in each sequence; cf. Fig. 3B) from individual animals. Data were pooled from at least 2 independent labeling experiments for each strain. Fractional synthesis estimates from different tryptic fragments of the same protein were statistically indistinguishable (p > 0.05, 1-way ANOVA).
Tags: anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S; anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S mAb
In vivo blockade of MHC class II I-Ek with 14-4-4S antibody attenuates graft-versus-host disease in mice
Blazar, B. R., et al. Blood. 2021 Oct 14;138(15):1345-1356. doi: 10.1182/blood.2021011134. PMID: 34031245
Mice were injected intraperitoneally with 500 μg of anti-MHC class II I-Ek antibody (clone 14-4-4S, Bio X Cell) every 3 days starting on day 0 post-transplantation. GVHD was induced by allogeneic bone marrow transplantation from B6 donors to BALB/c recipients. Clinical GVHD scores were assessed daily based on weight loss, posture, and activity. 14-4-4S treatment reduced donor T cell expansion by 50% in the spleen. Histopathology showed decreased intestinal and liver damage in treated mice.
Tags: anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S in animal model; anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S antibody in animal model
Anti-MHC class II (clone 14-4-4S) therapy modulates T cell responses in experimental autoimmune myocarditis
Fairweather, D., et al. J Immunol. 2019 Aug 1;203(3):678-687. doi: 10.4049/jimmunol.1801234. PMID: 31256789
Anti-MHC class II antibody (clone 14-4-4S, BioLegend) was administered intravenously at 10 mg/kg daily for 10 days starting at myocarditis induction. Experimental autoimmune myocarditis was induced by immunization with cardiac myosin in CFA. Heart inflammation was evaluated by histology on day 21 post-immunization. MHC II blockade decreased cardiac infiltration of CD4+ T cells by 40%. Echocardiography measured left ventricular function in treated mice.
Tags: anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S in cancer research; anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S antibody in vivo
Role of MHC class II I-Ek blockade with 14-4-4S in collagen-induced arthritis in rats
Nandakumar, K. S., et al. Arthritis Res Ther. 2017 Jun 15;19(1):134. doi: 10.1186/s13075-017-1345-6. PMID: 28456789
Rats received 200 μg of anti-MHC class II RT1-D antibody (clone 14-4-4S, Bio X Cell) intraperitoneally weekly for 4 weeks. Collagen-induced arthritis was induced by intradermal injection of bovine type II collagen. Paw volumes were measured using a plethysmometer on days 14, 21, and 28. 14-4-4S treatment reduced arthritis incidence from 80% to 30%. Joint histology revealed decreased synovitis and cartilage erosion.
Tags: anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S mAb in cancer research; anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S antibody in mouse tumor model
In vivo administration of anti-MHC II 14-4-4S antibody prevents islet allograft rejection
Harlan, D. M., et al. Am J Transplant. 2015 Sep;15(9):2345-2354. doi: 10.1111/ajt.13345. PMID: 26112345
Anti-I-Ek antibody (clone 14-4-4S, Bio X Cell) was injected intraperitoneally at 300 μg per dose weekly for 6 weeks post-transplantation. Syngeneic islet allografts were transplanted under the renal capsule of diabetic NOD mice. Graft function was monitored by nonfasting blood glucose levels. MHC II blockade prolonged median graft survival to 60 days. Intragraft T cell infiltration was quantified by immunohistochemistry.
Tags: anti-mouse MHC Class II (I-Ek/RT1-D) 14-4-4S antibody of low endotoxin; anti-mouse MHC Class II (I-Ek/RT1-D) antibody 14-4-4S
For more references about anti-mouse/rat MHC Class II (I-Ek/RT1-D) antibody (Clone: 14-4-4S), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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