To produce hapten-carrier conjugates for immunization of laboratory animals.
- IA000032-1008: Biotin-Capture Beads
The Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical Stability. The modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications. - IA000033-1009
The Mono-Avidin Beads enables mild affinity purification of biotinylated proteins, peptides and other ligands. The mono-avidin molecules immobilized on the beads have a much lower biotin-binding affinity than native avidin thus enabling dissociation of biotinylated molecules using free biotin molecules or low pH buffer. In addition, the Mono-Avidin Beads can be regenerated at least 10 times with no significant loss in binding capacity. - IA000034-1011: Imino-Biotin Beads
The Imino-Biotin Beads enables mild affinity purification of various proteins belonging to the Avidins family. The Imino-Biotin molecules immobilized on the beads are capable of binding Avidins at neutral and basic pH and eluting in under acidic conditions. The Imino-Biotin Beads can be regenerated at least 10 times with no significant loss in binding capacity. - IA000035-1031
Goat Anti-Rabbit IgG (whole molecule) was affinity purified on Rabbit IgG affinity column using IgG isolated from pooled normal Rabbit sera. Eluted antibodies were further purified by Protein G column in order to separate IgGs from other classes of antibodies. Purified Antibodies were coupled to Sepharose Beads using TargetLock Chemistry. - IA000036-1032
Goat Anti-Mouse IgG (whole molecule) was affinity purified on Mouse IgG affinity column using IgG isolated from pooled normal Mouse sera. Eluted antibodies were further purified by Protein G column in order to separate IgGs from other classes of antibodies. Purified Antibodies were coupled to Sepharose Beads using TargetLock Chemistry. - IA000037-1029
Polyacrylamide gel electrophoresis (PAGE) has proven to be a useful tool for the separation of protein subunits and the determination of their molecular masses. Biotinylated Protein Size-Markers enables detection of size markers through biotin. For example HRP-Streptavidin may be used for the detection of the marker bands on blot or on film. The proteins included in Biotinylated Protein Size-Markers provide a suitable molecular mass range for most applications. Staining markers with Brilliant Blue R stain is possible. Protein bands are approximately equal in color intensity. - IA000038-B-9007-105K
* Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
* Fast and easy method to biotinylate and determine the amount of labeling
* Label and determine the extent of biotin labeling with one reagent
* Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
* Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. - IA000039-A-9002-001
HRP-IgG conjugates in just over 4 hr
Includes columns to easily deliver purified conjugates
Gentle chemistry: Leaves antibodies intact, no reducing reagents
Easy-to-use protocols and calculators on flash drive
Compatible with Western, ELISA and IHC applications
This is the only kit on the market that can guarantee 100% conversion of antibody to conjugate, a purified conjugate free of any unlabeled antibody or enzyme and a high final yield of > 50% - IA000040-M-1002-010
The high surface area and lower non-specific binding of NanoLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate.
IA000040-M-1003-010
The high surface area and lower non-specific binding of MagnaLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate. - IA000041-M-1002-010
The high surface area and lower non-specific binding of NanoLink? Streptavidin Magnetic Beads are ideal for immobilizations and Co-IP applications. These microspheres are particularly suited for high throughput robotic applications where high biotin loads must be removed or immobilized without the presence of an iron leachate.
80 Activated Carrier Proteins Products