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Conditions of optimal G-CSF protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Introduction
Human Granulocyte-Colony-Stimulating Factor (G-CSF), a 20 kDa glycoprotein containing internal disulfide bonds, induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. G-CSF functions through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT), Ras/mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. Bacterial endotoxins, TNF, Interleukin-1, and GM-CSF induce the synthesis of G-CSF while prostaglandin E2 inhibits its synthesis. Interleukin-17 induces the secretion of G-CSF in epithelial, endothelial, and fibroblastic cells.
BP000175-GD16: Recombinant Human Granulocyte colony-stimulating factor (G-CSF) Protein
Source: E. coli-derived.
N-terminal sequence analysis: Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser- Phe-Leu-leu.
Predicted molecular mass: 20 kDa, the 174-amino acid form of human G-CSF.
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is < 0.1 ng/ml, corresponding to a specific activity of 6.0x10^7 IU/mg.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.01 ng/ug (0.1 IEU/ug).
Formulation: In 0.2 um filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0.
Shipping: The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt if stored at 4°C as supplied.
Other G-CSF Proteins:
Recombinant human Pegylated G-CSF protein
Recombinant human G-CSF protein with His-tag
Recombinant human G-CSF protein produced in HEK
Recombinant human G-CSF protein produced in CHO
Recombinant mouse G-CSF protein
There are two forms, 174- and 180-amino-acid, of the natural human G-CSF protein. The 174-amino acid form is more abundant and active than the longer form and widely used in research and therapeutics. The structure of the recombinant human G-CSF protein expressed in E. coli, such as filgrastim, differs slightly from those of the natural glycoprotein and the recombinant human G-CSF protein expressed in mammalian cells such as CHO cells. PEGylation (polyethylene glycol) significantly extends half-life of the recombinant human G-CSF protein.
G-CSF Antibodies:
G-CSF monoclonal antibody
G-CSF monoclonal antibody (FITC-conjugated)
G-CSF monoclonal antibody (Biotin-conjugated)
G-CSF ELISA Kits:
Human G-CSF ELISA kit
Mouse G-CSF ELISA kit
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