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Home > Services > Antibody Services > Antibody Engineering Services
Antibody variable region cloning and sequencing, recombinant IgG and scFv-Fc construction and production, antibody humanization, and antibody optimization.
    10 Antibody Engineering Services
  • Single B cell antibody cloning and sequencing has become popular for antibody discovery, especially for those difficult to obtain.  After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by Sanger DNA sequencing or next generation sequencing (NGS) has been successfully applied to single B cell antibody technologies.

  • 100000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.

    - Reliable results. With optimized workflow and PCR primers, we have successfully cloned and sequenced >3000 antibodies from various species in the past 10 years. Syd Labs has become a reliable CRO supplier of antibody sequencing to biotech and biopharma companies, government agencies including FDA and NIH, and universities. Clients' samples of hybridoma cell lines or clonal B cells just need to meet 2 requirements: functional antibodies are secreted by the cells; enough RNA remains.
    - Save time. One may learn how to clone and sequence antibody genes by studying hybridoma antibody cloning and antibody sequencing protocols that we collected from public literature. Generally speaking, it is more difficult to clone antibody genes from rodents than other species; among isotypes, it is the easiest to clone IgG genes, especially IgG1. A couple of companies sell such kits and PCR primers. However, it takes time to become a highly efficient and experienced professional for so many lab skills and bioinformatics analysis involved in such projects. In addition, there are some samples invaluable but difficult to work. Some service suppliers spend months but could not solve the problem, just wasting clients' samples and time.
    - Save money. Since we process samples from multiple clients in the same time, our cost is lower than clients'. Our low cost molecular biology reagents further save clients' cost.

  • Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.

    A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.

    Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.

  • Fast turnaround (BS079A, B, and C):

    * 1-2 weeks for codon optimization and synthesizing antibody variable regions.
    * 1-2 weeks for molecular construction of recombinant scFv-Fc antibodies using E.coli.
    * 1-2 weeks for expressing and purifying recombinant scFv-Fc antibodies using HEK 293 cells.

    Protein A is used for recombinant antibody purification. Please request if multiple purification methods are required.

    * BS079D: 0.03 liter transient production, estimated yield 0.5 - 12 mg, average 1.5 mg, 2 weeks;
    * BS079E: 0.1 liter transient production, estimated yield 3 - 110 mg, average 14 mg, 2 weeks;
    * BS079F: 0.5 liter transient production, estimated yield 12 - 300 mg, average 75 mg, 2 weeks;
    * BS079G: 2 liter transient production, estimated yield 50 - 2000 mg, average 250 mg, 3 weeks;
    * BS079H: 10 liter transient production, estimated yield 120 - 5000 mg, average 1100 mg, 3 weeks.

  • Fast turnaround (BS046A, B, and C):

    * 1-2 weeks for codon optimization and synthesizing antibody variable regions.
    * 1-2 weeks for molecular construction of recombinant IgG antibodies using E.coli.
    * 1-2 weeks for expressing and purifying recombinant IgG antibodies using HEK 293 cells.

    Protein A is used for recombinant antibody purification. Please request if multiple purification methods are required.

    * BS046D: 0.03 liter transient production, estimated yield 0.5 - 10 mg, average 2 mg, 2 weeks;
    * BS046E: 0.1 liter transient production, estimated yield 2 - 50 mg, average 10 mg, 2 weeks;
    * BS046F: 0.5 liter transient production, estimated yield 10 - 250 mg, average 50 mg, 2 weeks;
    * BS046G: 2 liter transient production, estimated yield 40 - 2000 mg, average 200 mg, 3 weeks;
    * BS046H: 10 liter transient production, estimated yield 200 - 5000 mg, average 1000 mg, 3 weeks.

  • Our FcRγ binding assay and FcRn binding assay services include: his-tagged receptors are immobilized onto anti-His biosensors; the loaded biosensors are dipped into a dilution series of the antibody; the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated.

  • Syd Labs screens scFv phage libraries using several different formats for applications including de novo antibody discovery and affinity maturation of existing antibodies.

  • Syd Labs generates scFv phage libraries from immunized or naive donors from several species. Additionally, various mutagenesis strategies can be used to design scFv phage libraries for affinity maturation of a given antibody or other optimization purposes. Our scFv phage library is generated with a goal of up to 1010 clones and quality controlled by sequencing 96 clones at random.

  • Our epitope binning service include: Antigen is immobilized onto a biolayer interferometry (BLI) sensor using an appropriate method. Antibody 1 is allowed to saturate its epitope on the antigen. Binding of Antibody 2 to antigen saturated with Antibody 1 is assessed.

    Fast turnaround: 2-3 weeks.

  • Fast turnaround: 3-6 months with our proprietary optimization algorithm.

    Antibody affinity can be improved by about 10 folds after each round of antibody affinity maturation.

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