Single B cell antibody cloning and sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by next generation sequencing (NGS) including nanopore sequencing has been successfully applied to single B cell antibody technologies.
20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
BS046B:
Gene synthesis of the variable regions of the recombinant IgG antibodies;
Molecular construction of the recombinant IgG antibodies;
0.04 Liter transient production of the recombinant IgG antibodies in CHO cells;
Protein A purification.BS079B:
Gene synthesis of the variable regions of the recombinant scFv-Fc antibodies;
Molecular construction of the recombinant scFv-Fc antibodies;
0.04 Liter transient production of the recombinant scFv-Fc antibodies in CHO cells;
Protein A purification.Fast turnaround: 8-10 weeks in total. Our antibody humanization process involves significant computational analysis, and thus is quite efficient and requires much less trial time.
A human antibody database and a proprietary method are used for analysis and design of humanized antibodies.
Nine humanized antibodies (each combination of 3 designed heavy chains and 3 designed light chains) are constructed and produced for antigen binding confirmation and affinity measurement.
Syd Labs screens scFv phage libraries using several different formats for applications including de novo antibody discovery and affinity maturation of existing antibodies.
Syd Labs generates scFv phage libraries from immunized or naive donors from several species. Additionally, various mutagenesis strategies can be used to design scFv phage libraries for affinity maturation of a given antibody or other optimization purposes. Our scFv phage library is generated with a goal of up to 1010 clones and quality controlled by sequencing 96 clones at random.
Fast turnaround: 3-6 months with our proprietary optimization algorithm.
Antibody affinity can be improved by about 10 folds after each round of antibody affinity maturation.