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Home > Proteins & Peptides > A-Z Proteins  > Biotinylated Human PD-1 Protein

Biotinylated Human PD-1 Protein

PDCD1; PD1; CD279; SLEB2; PD-1

Catalog No. Product Name Size List Price (US$) Quantity
BP004987 Biotinylated Human PD-1/PDCD1 Protein with the C-His-Avi Tag 100 ug 500.00
BP004987 Biotinylated Human PD-1/PDCD1 Protein with the C-His-Avi Tag 500 ug 1750.00
BP005587 Biotinylated Human PD-1/PDCD1 Protein with the C-hFc-Avi Tag 100 ug 500.00
BP005587 Biotinylated Human PD-1/PDCD1 Protein with the C-hFc-Avi Tag 500 ug 1750.00
Description

BP004987: Biotinylated Human PD-1/PDCD1 Protein with the C-His-Avi Tag

Source: The recombinant Biotinylated Human PD-1/PDCD1 Protein, (amino acids Leu25-Gln167) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human PD-1/PDCD1 protein with the His-Avi tag at the C-terminus has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Biotinylated Human PD-1 protein with the C-His-Avi tag at 1 ug/ml (100 ul/Well) on streptavidin (5 ug/ml) precoated plate. Dose response curve for the recombinant Human PD-L1 protein with the C-hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.356 uM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human PD-1/PDCD1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.

BP005587: Biotinylated Human PD-1/PDCD1 Protein with the C-hFc-Avi Tag

Source: The recombinant Biotinylated Human PD-1/PDCD1 Protein, (amino acids Leu25-Gln167) fused with a hFc-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Biotinylated Human PD-1/PDCD1 protein with the hFc-Avi tag at the C-terminus has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result.
Activity: Immobilized recombinant Human PD-L1 protein with the C-hFc-Avi tag at 2 ug/ml (100 ul/Well). Dose response curve for the recombinant Biotinylated Human PD-1 protein with the C-hFc tag with the EC50 of 0.23 ug/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Biotinylated Human PD-1/PDCD1 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.


Shipping: The recombinant Biotinylated Human PD-1/PDCD1 Protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 week, 2 to 8°C under sterile conditions after reconstitution.
6 months, -20 to -70°C under sterile conditions after reconstitution.

Syd Labs also provides the following PD-1/PDCD1 Protein:
Biotinylated Mouse PD-1 Protein
Human PD-1/PDCD1 Protein
Mouse PD-1/PDCD1 Protein
Cynomolgus PD-1/PDCD1 Protein
Canine PD-1/PDCD1 Protein
Human VEGF Protein
Human IL-2 Protein

Syd Labs provides the following anti-mouse PD-L1 / PD-1 antibodies:
In vivo grade recombinant anti-mouse PD1 antibodies (Clone 29F.1A12.1)
In vivo grade recombinant anti-mouse PD-1 antibodies (Clone RMP1-14.1)
In vivo grade recombinant anti-mouse PD-L1 antibodies (Clone 10F.9G2.1)

Syd Labs also provides the following PD-L1/PD-L2 Protein:
Human PD-L1/B7-H1 Protein
Cynomolgus PD-L1/B7-H1 Protein
Mouse PD-L1/B7-H1 Protein
Human PD-L2/B7-DC Protein
Biotinylated Human PD-L1 Protein

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