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Probe qPCR Master Mixes

Probe qPCR PreMix, qPCR MasterMix, qPCR SuperMix

Catalog No. Product Name Size List Price (US$) Quantity
MB148 2x Universal U+ Probe qPCR Master Mix, Antibody-Based 500 reactions (20 ul/reaction) 400.00
MB148 2x Universal U+ Probe qPCR Master Mix, Antibody-Based 2500 reactions (20 ul/reaction) 1600.00
MB149 2x Universal Probe qPCR Master Mix, Antibody-Based 500 reactions (20 ul/reaction) 360.00
MB149 2x Universal Probe qPCR Master Mix, Antibody-Based 2500 reactions (20 ul/reaction) 1400.00
MB150 2x U+ Probe qPCR Master Mix, Antibody-Based 500 reactions (20 ul/reaction) 320.00
MB150 2x U+ Probe qPCR Master Mix, Antibody-Based 2500 reactions (20 ul/reaction) 1350.00
MB151 2x Probe qPCR Master Mix, Antibody-Based 500 reactions (20 ul/reaction) 300.00
MB151 2x Probe qPCR Master Mix, Antibody-Based 2500 reactions (20 ul/reaction) 1250.00
MB155 2x Universal U+ Probe qPCR Master Mix, Chemical-Based 500 reactions (20 ul/reaction) 360.00
MB155 2x Universal U+ Probe qPCR Master Mix, Chemical-Based 2500 reactions (20 ul/reaction) 1400.00
MB156 2x U+ Probe qPCR Master Mix, Chemical-Based 500 reactions (20 ul/reaction) 300.00
MB156 2x U+ Probe qPCR Master Mix, Chemical-Based 2500 reactions (20 ul/reaction) 1250.00
MB157 2x Probe qPCR Master Mix, Chemical-Based 500 reactions (20 ul/reaction) 280.00
MB157 2x Probe qPCR Master Mix, Chemical-Based 2500 reactions (20 ul/reaction) 1100.00
Description

MB148: Universal U+ Probe qPCR Master Mix, Antibody-Based

The universal U+ probe qPCR master mix, antibody-based is ready-to-use 2x concentrated solution for quantitative PCR by the probe method. Except DNA template and primers, the 2x universal U+ probe qPCR master mix, antibody-based contains all other components for qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, heat-labile UDG, and antibody-based hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 10 ul of 2x universal U+ probe qPCR master mix, antibody-based to specific primers, DNA template, and water. 

Using the Taq DNA polymerase specific antibodies,  the universal U+ probe qPCR master mix gains the advantages of high specificity, high detection sensitivity, and high amplification yield. Combined with the optimal buffer optimized for qPCR and the specific promoting factors, the universal U+ probe qPCR master mix greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. The universal U+ probe qPCR master mix, antibody-based contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments. The the universal U+ probe qPCR master mix contains a dUTP/UDG system that can prevent the contamination of PCR product. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR.

Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.

Features of the Universal U+ Probe qPCR Master Mix, Antibody-Based:
* High detection sensitivity.
* More stable amplification curve with low concentration template.
* Reduced formation of primer dimers and improved specificity of qPCR products.
* Convenient, easy, and quick operation.

Components of the Universal U+ Probe qPCR Master Mix, Antibody-Based:
2x universal U+ probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, specific enhancer factors, heat-labile UDG, and specific ROX reference dye.

Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Components of the Universal Probe qPCR Master Mix, Antibody-Based (Catalog No. MB149):
2x universal probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, specific enhancer factors, and specific ROX reference dye.

Components of the U+ Probe qPCR Master Mix, Antibody-Based (Catalog No. MB150) :
2x U+ probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, heat-labile UDG, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.

Components of the Probe qPCR Master Mix, Antibody-Based (Catalog No. MB151) :
2x probe qPCR master mix: Mixture of antibody-based hot start Taq DNA polymerase, dNTP mix, MgCl2, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.

MB155: Universal U+ Probe qPCR Master Mix, Chemical-Based

The universal U+ probe qPCR master mix, chemical-based is ready-to-use 2x concentrated solution for quantitative PCR by the probe method. Except DNA template and primers, the 2x universal U+ probe qPCR master mix, chemical-based contains all other components for qPCR, including dNTP/dUTP mix, MgCl2, reaction buffer, heat-labile UDG, and chemically modified hot start Taq DNA polymerase at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul reaction, simply add 10 ul of 2x universal U+ probe qPCR master mix, chemical-based to specific primers, DNA template, and water. 

Using the chemically modified Taq DNA polymerase,  the universal U+ probe qPCR master mix gains the advantages of high specificity, high sensitivity, and high amplification efficiency. Combined with the optimal buffer optimized for qPCR and the specific promoting factors, the universal U+ probe qPCR master mix greatly improves the qPCR amplification yield, with a ultra-high plateau value, and is suitable for qPCR amplification with high specificity and sensitivity. The universal U+ probe qPCR master mix contains a dUTP/UDG system that can prevent the contamination of PCR product. Heat-labile UDG can quickly eliminate the contamination of dU-containing products at room temperature. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. The universal U+ probe qPCR master mix, chemical-based contains a unique ROX Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.

Optimal template and primer concentrations, and qPCR program parameters should be determined experimentally by the investigator.

Features of the Universal U+ Probe qPCR Master Mix, Chemical-Based:
* Universal on all platforms: Special ROX reference dye suitable for all qPCR instruments; no need to adjust ROX concentration on different qPCR instruments.
* Rigorous hot-start enzyme: Chemically modified Taq DNA Polymerase providing high amplification specificity.
* Optimized reaction buffer formula: Minimizing non-specific amplification and primer dimerization.
* High amplification performance: repeatable and reliable quantitative results.

Components of the Universal U+ Probe qPCR Master Mix, Chemical-Based:
2x universal U+ probe qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, specific enhancer factors, heat-labile UDG, and specific ROX reference dye.

Storage/Stability: Stable for 1 year at -70°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

Components of the U+ Probe qPCR Master Mix, Chemical-Based (Catalog No. MB156) :
2x U+ Probe qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP/dUTP mix, MgCl2, heat-labile UDG, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.

Components of the Probe qPCR Master Mix, Chemical-Based (Catalog No. MB157) :
2x probe qPCR master mix: Mixture of chemically modified hot start Taq DNA polymerase, dNTP mix, MgCl2, and specific enhancer factors.
50x ROX reference dye 1.
50x ROX reference dye 2.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.

PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.

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