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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Both His-tagged and Fc-fused recombinant human and cyno ACE2 protein (rhACE2-His, rhACE2-Fc, and rcACE2-His) are available with an Avi site, which is for biotinylation. Please ask for a quote if you need protein mutants or a protein with different modification or components.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Source: Biotinylated recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a His tag and Avi at the C-terminus.
Accession: Q9BYF1
Predicted molecular mass: 86.5 kDa. Due to glycosylation, the biotinylated recombinant human ACE2 protein migrates to 95-110 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Activity: Immobilized recombinant SARS-CoV-2 (2019-nCoV) Spike S RBD at 1 ug/ml (100 ul/well). Dose response curve for the biotinylated recombinant human ACE2 protein with the EC50 of 0.1 ug/ml determined by ELISA. Conditions of optimal biotinylated recombinant Human ACE2 protein (rhACE2-His, rhACE2-Fc, and rcACE2-His) performance should be determined experimentally by the investigator.
Formulation: The biotinylated recombinant human ACE2-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.
Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.
Background
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services
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