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High Yield PCR Master Mix

PCR PreMix, PCR MasterMix, PCR SuperMix, Taq Master Mix

Catalog No. Product Name Size List Price (US$) Quantity
MB040-HY2-L 2x HY PCR Master Mix (Blue Dye) 1000 x 20 ul reactions 160.00
MB040-HY2-S 2x HY PCR Master Mix (Blue Dye) 100 x 20 ul reactions 26.00
Description

MB040-HY2: 2x HY PCR Master Mix, with blue dye

The high yield 2x PCR Master Mix with non-interfering blue dye (catalog No. MB040-HY2) carries a blend of Taq enzyme and a proofreading enzyme with 3′→5′ exonuclease activity. Compared to the 2x PCR Premixes (catalog No. MB067-EQ2) with the standard Taq enzyme only, the high yield 2x PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates. Except DNA template and primers, the high yield PCR master mix contains all other components for PCR, including a blend of Taq DNA polymerase and a proofreading enzyme, dNTPs, MgCl2, reaction buffer, PCR stabilizer and enhancer at optimal concentrations for consistent and efficient PCR amplification. For a 20 ul PCR reaction, simply add 10 ul of 2x HY PCR Master Mix to specific primers, DNA template, and water. No additional loading buffer is required for electrophoresis when the PCR reaction is completed.

Optimal template and primer concentrations, and PCR program parameters should be determined experimentally by the investigator.

Features of the high yield PCR master mix:
* The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
* The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
* The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.
* Features of the high yield Taq DNA polymerase (catalog No. MB016-HYT) contained in the high yield PCR master mix.
* Low cost: $160 for 10 ml 2x HY PCR Master Mix with blue dye. The cheapest high yield PCR master mix in the market.

Applications of the high yield PCR master mix:
* Routine PCR using genomic, viral and plasmid templates.
* High sensitivity PCR, including difficult, low copy, or “dirty” templates.
* DNA cloning and subcloning.
* High throughput PCR.
* DNA and colony screening.
* Genotyping.
* DNA mutagenesis.
* Long PCR (up to 10 kb human genomic and 15 kb lamda DNA fragments tested).

The high yield Taq DNA polymerase (catalog No. MB016-HYT) contained in the 2x high yield PCR PreMix is available alone.

Storage/Stability: Stable for 1 year at -20°C and 1 week at 4°C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Bst DNA polymerase large fragment.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR preMix with dye (green, red, blue).
* 2x rapid PCR master mix with a green dye.
* 2x long PCR master mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT supermix for qPCR.
* RT-PCR Kit.
* Dye-based one-step qRT-PCR kits.
* Probe-based one-step qRT-PCR kits.
* Probe-based multiplex one-step qRT-PCR kits.
* SYBR Green qPCR master mixes.
* Probe qPCR master mixes.

PCR components and related products:
* dNTP mix, 10 mM each and dNTP mix, 25 mM each.
* Proteinase K powder and Proteinase K solution.
* Reverse transcriptases and First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* Uracil DNA glycosylases.
* PCR enhancer.

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