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Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human HLA Class I Heavy Chain Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells.
Clone: HC10.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: HC10) specifically binds to human HLA Class I Heavy Chain.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human HLA Class I Heavy Chain protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human HLA Class I Heavy Chain antibodies (clone of HC10) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Gemcitabine Modulates HLA-I Regulation to Improve Tumor Antigen Presentation by Pancreatic Cancer Cells
Larson, A.C., et al. (2024). Int. J. Mol. Sci. 25(6):3211. doi: 10.3390/ijms25063211. PMID: 38537988
The HC10 antibody (anti-human HLA Class I Heavy Chain) was used to detect HLA-B and HLA-C heavy chains in western blot and flow cytometry analyses of pancreatic cancer cell lines (S2-013, PANC-1). Gemcitabine treatment strongly increased HLA-B/C protein expression and surface levels, with similar amplification to HLA-A2, enhancing tumor antigen presentation despite a milder effect on HLA-A.
Tags: anti-human HLA Class I Heavy Chain HC10; anti-human HLA Class I Heavy Chain HC10 mAb
Endo-lysosomal assembly variations among human leukocyte antigen class I allotypes
Olson, E., et al. (2023). eLife. 12:e79144. doi: 10.7554/eLife.79144. PMID: 36722462
The HC10 antibody (anti-human HLA Class I Heavy Chain) detected peptide-deficient HLA-B and HLA-C heavy chain conformations in bead-based assays, showing reduced signals with high-affinity peptide loading for B08:01 and B35:01 allotypes. Flow cytometry revealed that HC10 signals did not increase with β2m loss, indicating internalization of heterodimeric HLA class I complexes in moDCs.
Tags: anti-human HLA Class I Heavy Chain HC10 in vivo; anti-human HLA Class I Heavy Chain HC10 in animal model
Antibody-mediated delivery of viral epitopes to tumors harnesses CMV-specific T cells for cancer therapy
Millar, D.G., et al. (2020). Nat Biotechnol. 38:420-425. doi: 10.1038/s41587-019-0404-8. PMID: 32015550
The HC10 antibody (anti-human HLA Class I Heavy Chain) was used to assess empty HLA molecules on Colo205 tumor cells via flow cytometry. After acid-stripping peptides from surface MHC molecules, HC10 staining showed a decrease in empty MHC molecules, supporting the use of antibody-peptide epitope conjugates (APECs) to retarget CMV-specific CD8+ T cells for tumor therapy.
Tags: anti-human HLA Class I Heavy Chain HC10 antibody in cancer research; anti-human HLA Class I Heavy Chain HC10 in human tumor model
In vivo Downregulation of MHC Class I Molecules by HCMV Occurs During All Phases of Viral Replication but Is Not Always Complete
Gabor, F., et al. (2020). Front Cell Infect Microbiol. 10:283. doi: 10.3389/fcimb.2020.00283. PMID: 32596168
The HC10 antibody (anti-human HLA Class I Heavy Chain), provided by Dr. H. L. Ploegh, detected HLA-B, HLA-C, and select HLA-A heavy chains (e.g., HLA-A10, A28–A33) in HCMV-infected cells via flow cytometry. It revealed strong but incomplete MHC class I downregulation, with some cells showing weak to normal HC10 signals compared to uninfected cells.
Tags: anti-human HLA Class I Heavy Chain HC10 antibody of low endotoxin; anti-human HLA Class I Heavy Chain (HC10) in vivo antibody
The amino acid at position 97 is involved in folding and surface expression of HLA-B27
Blanco-Gelaz, M.A., et al. (2006). Int Immunol. 18(1):211-20. doi: 10.1093/intimm/dxh364. PMID: 16361312
The HC10 antibody (anti-human HLA Class I Heavy Chain), provided by Dr. Ted Hansen and Dr. H. L. Ploegh, was used to detect β2m-free HLA-B and HLA-C heavy chains, including select HLA-A isoforms (e.g., HLA-A10, A28–A33). Flow cytometry confirmed that β2m-free heavy chains originate from surface β2m-associated MHC class I molecules, with position 97 affecting HLA-B27 folding and expression.
Tags: anti-HLA Class I Heavy Chain clone HC10; clone HC10 of human HLA Class I Heavy Chain antibody
For more references about anti-human HLA Class I Heavy Chain antibody (HC10), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2a isotype control antibody
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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