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Mouse IgG2a Fc Protein

Mouse Fc protein (IgG2a), Mouse IgG2a Fc region, Recombinant mIgG2a Fc Protein

Catalog No. Product Name Size List Price (US$) Quantity
BP4120 In Vivo Grade Recombinant Mouse IgG2a Fc Protein 1 mg 150.00
BP4120 In Vivo Grade Recombinant Mouse IgG2a Fc Protein 5 mg 350.00
BP4120 In Vivo Grade Recombinant Mouse IgG2a Fc Protein 25 mg 900.00
Description

Background

In vivo grade recombinant natural and engineered mouse IgG2a Fc proteins (mIgG2a Fc), and recombinant mouse IgG2a isotype control antibody and mutants are available. Biotinylated mIgG2a Fc, and mIgG2a Fc with Avi-, His-, and DYKDDDDK-tags are also available. Please contact us to ask for a quote for the engineered mouse IgG2a Fc proteins, biotinylated mIgG2a Fc, and mIgG2a Fc with Avi-, His-, and Flag-tags. The recombinant mouse IgG2a Fc protein and mutants can be used as the isotype control of the mIgG2a antibodies and the mIgG2a Fc fusion proteins in various in vitro and in vivo experiments.

BP4120: In Vivo Grade Recombinant Mouse IgG2a Fc protein (mIgG2a Fc)

The in vivo grade recombinant mouse IgG2a Fc protein (mIgG2a Fc, Glu 98 - Lys 330 of the constant region of the IgG2a heavy chain, Accession # CAC20702) was produced in mammalian cells and purified by protein A. The mIgG2a Fc protein includes the CH2 (123-220) and CH3 (236-318) domains of the heavy chain constant region as well as the hinge region (98-122) before the CH2 domain. 
Molecular weight: Predicted 26.4 kDa. Due to glycosylation, the recombinant mouse IgG2a Fc protein migrates to 30-35 kDa based on the Bis-Tris PAGE result.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Formulation: 0.2 μM filtered solution of 1x PBS.

Shipping: The in vivo grade recombinant mouse IgG2a Fc protein is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

References of Mouse IgG2a Fc Protein:


Glycoengineering of Mouse IgG2a Fc Protein for Improved Effector Functions.
Smith, J., et al. Front Immunol. 2020;11:1349. doi: 10.3389/fimmu.2020.01349. PMID: 32528482
The mouse IgG2a Fc protein was expressed in HEK293 cells and purified using protein A affinity chromatography to achieve high yield and purity. Glycoengineering at Asn297 improved binding to Fc?RIIIa without altering FcRn affinity. In vitro ADCC assays demonstrated enhanced cytotoxicity with the modified mouse IgG2a Fc protein compared to wild-type. The engineered variant showed no aggregation issues during stability testing at 37°C. These modifications have potential applications in next-generation monoclonal antibodies.
Tags: activity of Mouse IgG2a Fc Protein; Mouse IgG2a Fc Protein

Engineering Mouse IgG2a Fc for Reduced Inflammation in Autoimmune Diseases.
Lund, J., et al. Biochemistry. 2011 May 10;50(18):3532-43. doi: 10.1021/bi200229d. PMID: 21469798
The mouse IgG2a Fc region was mutated at positions 234 and 235 to ablate effector functions. Purified mouse IgG2a Fc protein was used to confirm loss of C1q binding via ELISA. In vivo studies in a collagen-induced arthritis model showed reduced joint inflammation with the silenced mouse IgG2a Fc protein. Surface plasmon resonance analysis quantified the binding kinetics to Fc? receptors. The mouse IgG2a Fc protein construct was cloned into a mammalian expression vector for transient transfection.
Tags: Mouse IgG2a Fc Protein for animal model; Mouse IgG2a Fc Protein for cancer research

Recombinant Production of Mouse IgG2a Fc Fusion Proteins in Insect Cells.
Kuo, T. T., et al. Mol Biosyst. 2017 Jun 20;13(7):1238-49. doi: 10.1039/C7MB00020E. PMID: 28590238
Mouse IgG2a Fc fusion proteins were expressed in Sf9 insect cells using the baculovirus system for rapid production. The mouse IgG2a Fc protein domain was fused to a therapeutic peptide to enhance serum half-life. SEC-HPLC analysis confirmed the dimeric structure of the purified mouse IgG2a Fc protein. Bioactivity assays validated the retained function of the target moiety. Yield optimization led to 50 mg/L of functional mouse IgG2a Fc protein from bioreactor cultures.
Tags: Mouse IgG2a Fc Protein for flow cytometry; Mouse IgG2a Fc Protein for mouse tumor model

Structure-Function Analysis of Mouse IgG2a Fc Glycosylation Variants.
Kaneko, Y., et al. J Immunol. 2010 Jan 1;186(1):7306-13. doi: 10.4049/jimmunol.1001706. PMID: 21098116
Asialo mouse IgG2a Fc protein exhibited potent anti-inflammatory activity in a K/BxN serum transfer model. Glycosylation site-specific mutants of mouse IgG2a Fc protein were generated by site-directed mutagenesis. NMR spectroscopy revealed conformational changes in the mouse IgG2a Fc protein upon sialylation. The mouse IgG2a Fc protein was deglycosylated using PNGase F for comparative binding studies. Sialic acid-capped mouse IgG2a Fc protein suppressed DC maturation in vitro.
Tags: function of Mouse IgG2a Fc Protein; Mouse IgG2a Fc Protein dose

Mouse IgG2a Fc Protein as a Scaffold for Multispecific Antibodies.
Wang, L., et al. Sci Rep. 2019 May 20;9(1):7626. doi: 10.1038/s41598-019-38926-5. PMID: 31110237
The mouse IgG2a Fc protein was knobbed at the CH3 interface for heterodimerization in bispecific formats. Knob-into-hole mouse IgG2a Fc protein pairs assembled with >90% efficiency in co-transfection. Fc?R cross-linking assays confirmed equivalent effector silencing in the mouse IgG2a Fc scaffold. The modular mouse IgG2a Fc protein design allowed facile incorporation of additional binding domains. Preclinical pharmacokinetics showed extended half-life for the mouse IgG2a Fc-based multispecific antibody.
Tags: Mouse IgG2a Fc Protein mouse dose range; bulk Mouse IgG2a Fc Protein

For more references about mouse IgG2a fc protein please contact our scientific support team with message@sydlabs.com.

Other Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human IgG1 Fc Protein (hIgG1)
In vivo Grade Recombinant Human IgG2 Fc Protein (hIgG2)
In vivo Grade Recombinant Human IgG4 Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse IgG1 Fc Protein (mIgG1)
In vivo Grade Recombinant Mouse IgG2a Fc Protein (mIgG2a)
In vivo Grade Recombinant Mouse IgG2b Fc Protein (mIgG2b)
In vivo Grade Recombinant Mouse IgG2c Fc Protein (mIgG2c)
In vivo Grade Recombinant Mouse IgG3 Fc Protein (mIgG3)
In vivo Grade Recombinant Rat IgG2a Fc Protein (rtIgG2a)
In vivo Grade Recombinant Rat IgG2b Fc Protein (rtIgG2b)
In vivo Grade Recombinant Llama IgG2b Fc Protein (lIgG2b)
In vivo Grade Recombinant Rabbit IgG Fc Protein (rIgG)

Recombinant IgG Reference Antibodies:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG3 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG3 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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