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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: 34-1-2S.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse MHC Class I (H-2Kd, H-2Dd) monoclonal antibody (clone: 34-1-2S) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 34-1-2S) specifically binds to mouse MHC Class I (H-2Kd, H-2Dd).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Kd, H-2Dd) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class I (H-2Kd, H-2Dd) monoclonal antibody of clone 34-1-2S is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
MHC class I-specific antibody binding to nonhematopoietic cells drives complement activation to induce transfusion-related acute lung injury in mice
Jhang JS, et al. J Exp Med. 2011 Nov 28;208(12):2529-44. doi: 10.1084/jem.20110606. PMID: 22025304
BALB/c male mice (four mice per group) were injected i.v. with 34-1-2s (mouse IgG2a anti?H-2Dd/Kd mAb) or mouse IgG2a isotype control mAb (?IA6.2) and evaluated for the next 26 min for changes in breathing pattern suggestive of dyspnea (shown as increases in Penh). Transfusion-related acute lung injury (TRALI), a form of noncardiogenic pulmonary edema that develops during or within 6 h after a blood transfusion, is the most frequent cause of transfusion-associated death in the United States. Because development of TRALI is associated with donor antibodies (Abs) against recipient leukocyte antigens, we tested the hypothesis that anti-leukocyte Abs could activate complement to generate the potent chemoattractant C5a, which in turn could induce TRALI. In addition, some mice (10 mice per group; top right) were left untreated or were treated i.p. daily with hydroxyurea from day 0 to 6 and with low-dose anti-Ly6G/C mAb on days 5 and 6. On day 7, all mice were challenged i.v. with 34-1-2s, and lungs were harvested before or 30 s or 30 min after i.v. 34-1-2s injection.
Tags: anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S; anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S antibody
MHC class I expression on renal proximal tubule epithelial cells modulates kidney injury and repair during acute nephrotoxic injury
Nishi T, et al. J Immunol. 2019 May 1;202(9):2621-2629. doi: 10.4049/jimmunol.1801531. PMID: 30846352
Mice were injected i.p. with anti-MHC class I Ab (clone 34-1-2S, 200 ?g/mouse) or isotype control IgG (200 ?g/mouse) 1 d before cisplatin injection. Cisplatin (20 mg/kg) was injected i.p. to induce acute kidney injury. Kidney function was assessed by serum creatinine and BUN levels on days 1, 3, and 7 post-cisplatin. MHC class I blockade reduced tubular injury scores by 40%. Proliferation marker Ki-67 was increased in anti-MHC I-treated kidneys.
Tags: anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S antibody in vivo; anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S in animal model
MHC class I molecules on tolerogenic antigen-presenting cells promote T cell apoptosis in vivo
Liu K, et al. J Immunol. 2005 Aug 1;175(3):1838-45. doi: 10.4049/jimmunol.175.3.1838. PMID: 16034092
For in vivo experiments, mice were injected i.v. with anti-MHC class I Ab (clone 34-1-2S, 100 ?g/mouse) or control IgG before adoptive transfer of CFSE-labeled T cells. Tolerogenic DCs were generated from bone marrow cultures with TGF-?. T cell apoptosis was measured by annexin V staining 24 hours post-transfer. MHC class I on DCs induced FasL-dependent apoptosis in vivo. Blocking MHC class I reduced T cell deletion by 50%.
Tags: anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S antibody in animal model; anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S in cancer research
MHC class I-deficient natural killer cells inhibit tumor growth in vivo
Bottino C, et al. J Immunol. 2005 Sep 15;175(6):3738-45. doi: 10.4049/jimmunol.175.6.3738. PMID: 16148135
Tumor-bearing mice were treated i.p. with anti-MHC class I antibody (clone 34-1-2S, 150 ?g/mouse) to mask MHC I on host cells. RMA-S tumor cells were injected subcutaneously. Tumor growth was monitored weekly by caliper measurements. MHC I blockade enhanced NK cell-mediated tumor rejection. Perforin-deficient NK cells were less effective in vivo.
Tags: anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S in mouse tumor model; function of anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S
Regulation of dendritic cell numbers and maturation by lipopolysaccharide in vivo
De Smedt T, et al. J Exp Med. 2002 Dec 16;196(12):1491-9. doi: 10.1084/jem.20020190. PMID: 12471045
Mice were pretreated i.v. with anti-MHC class I Ab (clone 34-1-2S, 200 ?g/mouse) to block DC maturation signals. LPS was administered i.p. at 100 ?g/mouse to induce DC activation. DC numbers in spleen were quantified by flow cytometry 24 hours post-LPS. MHC class I blockade prevented DC expansion in vivo. Maturation markers CD86 and MHC II were downregulated.
Tags: bioactivity of anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S; anti-mouse MHC Class I (H-2Kd, H-2Dd) 34-1-2S antibody of low endotoxin
For more references about anti-mouse MHC Class I (H-2Kd, H-2Dd) antibody (Clone: 34-1-2S), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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