PA007199.m2a: In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Mouse IgG2a Kappa
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: 17A2.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
PA007199.r2b: In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Rat IgG2b Kappa
Recombinant rat IgG2b Monoclonal Antibody.
Clone: 17A2.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
References of anti-mouse CD3 antibody (17A2)
Creatine uptake regulates CD8 T cell antitumor immunity
Di Biase S, Ma X, Wang X, et al. (2019). J Exp Med. 2019 Dec 2;216(12):2869-2882. doi: 10.1084/jem.20182044. PMID: 31628186
Purified anti-mouse CD3 antibody (clone 145-2C11) used for in vitro stimulation of CD8 T cells was purchased from...Anti-mouse CD3 depleting antibody (clone 17A2) and its isotype control antibody (clone LTF-2), as well as anti-mouse PD-1 blocking antibody (clone RMP1-14) and its isotype control antibody (clone 2A3), used for in vivo animal study were purchased from...CD8 T cells were cultured in vitro in standard T cell culture medium comprising RPMI 1640 (10040), 10% FBS (F2442; Sigma-Aldrich), 1% penicillin-streptomycin-glutamine (10378016), 1% MEM Non-Essential Amino Acids Solution (11140050; Gibco), 1% Hepes (15630080; Gibco), 1% sodium pyruvate (100 mM; 11360070; Gibco), and 0.05 mM β-mercaptoethanol (M3148)...For in vivo study of creatine supplementation for cancer immunotherapy, B6 mice were inoculated with B16-OVA tumor cells and monitored for tumor growth, with or without receiving creatine supplementation via i.p. injection or diet...To study the combination effects of creatine supplementation and PD-1/PD-L1 blockade treatment, B6 mice were inoculated with MC38 tumor cells and monitored for tumor growth; experimental mice also received i.p. supplementation of creatine, as well as i.p. injection of an anti–PD-1 blocking antibody (clone RMP1-14; 300 μg/mouse/injection, twice per wk) or an isotype control antibody (clone 2A3; 300 μg/mouse/injection, twice per wk), alone or in combination.
Tags: anti-mouse CD3 17A2; anti-mouse CD3 17A2 antibody in vivo
In Vitro Priming of Adoptively Transferred T Cells with a RORγ Agonist Confers Durable Memory and Stemness In Vivo
Hu X, et al. (2018). Cancer Res. 2018 Jul 15;78(14):3888-3898. doi: 10.1158/0008-5472.CAN-17-3973. PMID: 29769201
Before probing with antibodies, FC block was applied to cells at 1 μg/100 μL...Antibodies used: anti-mCD3-efluor450 clone 17A2, IL-17A, and IFN-γ, anti-h/mRORγt-PE clone AFKJS-5019, anti-h/mCD44-PerCPCy5.5 clone IM7, anti-hCD4-APCH7 clone RPA-T4, anti-mCD4-APC/PE clone RM4-5, anti-mCD62L-APC clone MEL-14...As expected, neutralizing IL-17A or IFN-γ in mice infused with untreated cells (Figure 4A and 4B) or agonist-treated cells (Figure 4C and 4D) impaired their anti-tumor activity...To generate mesothelin-specific T cells, sorted pan T (CD4+ or CD8+) cells were activated with CD3/CD28 coated beads and programmed to a Th17 or Tc17 phenotype and then transduced with a chimeric anti-mesothelin single-chain variable fragment (scFv) fusion protein containing the T cell receptor ζ (TCR ζ) signaling domain and 4-1BB that was generated as described previously (13)...In both groups, IL-17A, IL-22 or IFN-γ were neutralized with an antibody 5 times every other day starting from the day of infusion...
Tags: anti-mouse CD3 17A2 in animal model; anti-mouse CD3 17A2 mAb in cancer research
Quantitative CD3 PET Imaging Predicts Tumor Growth Response to Anti-CTLA-4 Therapy
Larimer BM, et al. (2016). J Nucl Med. 2016 Oct;57(10):1607-1611. doi: 10.2967/jnumed.116.173930. PMID: 27230929
The monoclonal antimouse CD3 antibody clone 17A2 was purchased from...Conjugation of anti-CD3 antibody (17A2) with DFO resulted in 0.8 ± 0.2 chelates per antibody (Fig. 1A)...For Western blotting, the tumors were lysed in 1% sodium dodecyl sulfate and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis...Blots were transferred to nitrocellulose, blocked with 5% nonfat dry milk, and probed with the appropriate antibodies...Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded 10-μm slices of tumor to confirm the cellular location of CD3 expression.
Tags: anti-mouse CD3 17A2 in mouse tumor model; anti-mouse CD3 17A2
CD137/OX40 Bispecific Antibody Induces Potent Antitumor Activity that Is Dependent on Target Coengagement
Gaspar M, et al. (2020). Cancer Immunol Res. 2020 Jun;8(6):781-793. doi: 10.1158/2326-6066.CIR-19-0798. PMID: 32273279
DO11.10 cells expressing human or mouse CD137 were stimulated with coated anti-mouse CD3 antibody (clone 17A2 at 0.1 μg/mL) and mouse IL2 concentration in the supernatants was measured by ELISA...Human T cells were isolated from PBMCs using Miltenyi Enrichment Kits (Human CD3⁺, 130-096-535; Human CD4⁺, 130-096-533; Human CD8⁺, 130-096-495; and Mouse CD3⁺, 130-095-130) according to the manufacturer's instructions and activated overnight using CD3/CD28 Dynabeads (Life Technologies catalog numbers: Human, 11131D and Mouse, 11453D) at a 1:1 cell to bead ratio...CD3/CD28 beads were removed using a DynaMag-15 magnet and activated T cells were washed with T-cell media and stimulated with coated CD3 antibody [Human: R&D Systems (MAB100) clone UHCT1 at 2.5 μg/mL (total and CD4⁺ T cells) or 5 μg/mL (CD8⁺ T cells) or Mouse: clone 145-2C11 at 2.5 μg/mL] in the presence of FS120 or control antibodies for 3 days at 37°C, 5% CO₂...Balb/c mice (n = 15) inoculated with 10⁶ CT26 cells subcutaneously (s.c.) and treated with 1 mg/kg FS120 surrogate or controls every 2 days (Q2D) starting on day 13 post-tumor inoculation for three doses injected intraperitoneally (IP)...Balb/c mice (n = 6) treated with 10 mg/kg FS120 surrogate or controls every 2 days starting on day 1 for three doses injected intraperitoneally (IP).
Tags: function of anti-mouse CD3 17A2; anti-mouse CD3 17A2 of low endotoxin
In vivo Imaging Technologies to Monitor the Immune System
McCarthy CE, et al. (2020). Front Immunol. 2020 Jun 2;11:1067. doi: 10.3389/fimmu.2020.01067. PMID: 32582173
For PET imaging, CD3 has been used pre-clinically to observe T cell trafficking in bladder and mammary cancer, in addition to monitoring response to CTLA-4 therapy in colon cancer models...In these studies, full-length mouse mAbs were conjugated with 89Zr...The tracers were observed to accumulate in secondary lymphoid tissues including spleen, thymus, and lymph nodes, which is not unexpected due to the relative densities of CD3+ T cells in these organs...Upon ex vivo analysis, one study reported a relative depletion of CD4 T cells and an expansion of CD8 T cells after delivery of an 89Zr-anti-CD3 tracer (clone 17A2) in mice...Scintigraphy imaging with technetium-99m (99mTc) conjugated anti-CD3 antibody (17A2) has been utilized in humans and preclinical mouse models to monitor sites of inflammation, particularly for rheumatoid arthritis and transplant rejection.
Tags: anti-mouse CD3 17A2 mAb; anti-mouse CD3 (17A2)
For more references aboutanti-mouse CD3 antibody (17A2), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)
Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry