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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls and Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant mouse IgG2a Monoclonal Antibody.
Clone: 17A2.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Recombinant rat IgG2b Monoclonal Antibody.
Clone: 17A2.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3 protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Metabolic Dynamics of In Vitro CD8+ T Cell Activation.
Joy Edwards-Hicks, et al. Metabolites. 2020 Dec 28;11(1):12. doi: 10.3390/metabo11010012. PMID: 33379404
Primary CD8+ T cells were isolated from murine spleens and cultured in vitro in the presence of activating stimuli CD3, CD28, and IL2. After 48 h and 72 h, the culture medium was exchanged for a medium containing survival growth factor IL2. Samples were taken every 12 h. ...CD8+ T cells were activated at 1.5 × 106/mL for 48 h using plate-bound anti-CD3 (5 ?g mL?1; InVivoMAb anti-mouse CD3, BioXCell no. BE0002) and soluble anti-CD28 (0.5 ?g/mL; InVivoMAb anti-mouse CD28, BioXcell no. BE0015) in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal calf serum (Gibco), 4 mM L-glutamine, 1% penicillin?streptomycin, hrIL-2 (human recombinant interleukin 2, 100 U ml?1, Peprotech), and 55 ?M beta-mercaptoethanol at 37 °C in a humidified incubator containing atmospheric oxygen supplemented with 5% CO2. ...Following 48 h activation, every 24 h T cells were counted and plated back at 1.5 × 106 in RPMI 1640 medium as above (minus CD3 and CD28) to prevent cell density from getting too high due to increased proliferation. ...To identify the areas of metabolism that are most affected during the activation of CD8+ T cells, we used the sum of the absolute loading values from the PLS analysis and the KEGG (Kyoto Encyclopedia of Genes and Genomes) definition of metabolic pathways [10] as input for a gene set enrichment analysis (GSEA) (Figure 3). ...In case multiple metabolites in the database have the same sum formula or very similar mass, one measured FIA feature can be annotated with multiple metabolites (see methods for details).
Tags: anti-mouse CD3 17A2 in cancer research; anti-mouse CD3 17A2 mAb in cancer research
Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
Shuxian Hu, et al. PLoS One. 2014 Aug 18;9(8):e105219. doi: 10.1371/journal.pone.0105219. PMID: 25133679
Lymphocytes were stimulated in co-cultures using a mixture of monoclonal antibodies to CD3 (5 ?g/ml; clone 17A2) and CD28 (5 ?g/ml; clone 37.5; BioXcell, West Lebanon, NH). ...CD8 lymphocytes, activated by crosslinking CD3 and CD28 with monoclonal antibodies (Mab), inhibited NSC growth in a dose-dependent manner. ...No change in NSC BrdU uptake was observed when treated with anti-CD3 or -CD28 antibodies alone or when cultured with activated or unstimulated CD4 lymphocyte (data not shown). ...IFN-? levels in the NSC-CD8 lymphocytes co-cultures (at 1:10 ratio) reached concentrations of 30.4 ± 3.9 pg/ml, 72 h after CD3-CD28 antibody stimulation. ...We found that antigen-independent activation of CD4 T-cells, using CD3 and CD28 antibodies, did not induce detectable levels of IFN-?, explaining their lack of effect on NSCs.
Tags: anti-mouse CD3 17A2 antibody in mouse tumor model; bioactivity of anti-mouse CD3 17A2 antibody
For more references about Anti-mouse CD3 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3)
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)
Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3)
Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: KT3) for flow cytometry
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