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Great for research reagent and IVD material. SARS-CoV-2 nucleocapsid antibodies, immunoassay kits, custom antibody and kit development services are available. To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Source: recombinant SARS-CoV-2 nucleocapsid protein (Ser2-Ala419) was expressed in E.coli with a His tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 48.9 kDa.
Endotoxin: Less than 1 EU per ug by the LAL method.
Formulation: The recombinant SARS-CoV-2 (2019-nCoV) nucleocapsid protein-His-Avi is supplied as 0.22 um filtered solution in 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5.
Purity: > 95% by PAGE under reduced condition.
Shipping: The recombinant SARS-CoV-2 (2019-nCoV) nucleocapsid protein-His-Avi is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
Questions and answers:
Question: Are the nucleocapsid proteins between SARS-CoV-2 and SARS-CoV highly conserved? How about their epitope sequences?
Answer: The nucleocapsid protein from SARS-CoV shows a similarity with the SARS-CoV-2 homologue slightly above 90%. Comparison of the epitope sequences, AQFAPSASAFFGMSR, QFAPSASAFFGMSRIGM, PKGFYAEGSRGGSQASSR, YNVTQAFGRRGPEQTQGNF, and KHWPQIAQFAPSASAFF highlights that SARS-CoV scored 100% sequence identity with SARS-CoV-2 epitopes. It is the reason why the SARS-CoV nucleocapsid antibodies have been widely used in manufacturing of SARS-CoV-2 IgG/IgM antibody rapid test kits.
Question: Does it mean that there is false positive result if the nucleocapsid-based SARS-CoV-2 IgG/IgM antibody rapid test kit is used for clinical diagnostic test?
Answer: Yes, it is highly possible especially if the patient has been infected by other coronavirus(es).
Question: Then why are the nucleocapsid-based SARS-CoV-2 IgG/IgM antibody test kits still widely used?
Answer: The nucleocapsid protein is the most abundant protein in SARS-CoV-2 and is highly immunogenic, it is often used as a marker in diagnostic immunoassays in the ELISA and colloidal gold-based rapid test kits. Its sensitivity is high though crossactivity is also high.
Question: Do you have discount if we place bulk order of the recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid protein-His-Avi for IVD material?
Answer: Sure. We also sell monoclonal human and rabbit nucleocapsid antibodies as well as alpaca nucleocapsid VHH antibodies.
Biotinylated Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-His-Avi
Recombinant SARS-CoV-2 Spike RBD-Fc-Avi
Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-His-Avi Protein
Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein
Recombinant SARS-CoV-2 Spike S-Fc-Avi Protein
Biotinylated Recombinant SARS Spike RBD-His-Avi
Recombinant SARS Spike RBD-His-Avi
Recombinant SARS-CoV-2 Nucleocapsid Protein-His-Avi
Biotinylated Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-His-Avi Protein
Recombinant Human ACE2-Fc-Avi Protein
To check more Syd Labs SARS-CoV-2 (2019-nCoV) Antigen and Antibody Reagents and Discovery Services
Background
The virus causing Coronavirus Disease 2019 (COVID-19) was named 2019 novel coronavirus (2019-nCoV) or severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Similar to those of other coronaviruses, there are four major structural proteins in SARS-CoV-2, S protein (spike protein), E protein (envelope protein), M protein (membrane protein), and N protein (nucleocapsid protein). The main function of Nucleocapsid protein is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells.
The nucleocapsid (N) phosphoprotein is the most abundant protein in coronavirus. Because the N protein is highly immunogenic and conserved among cornnaviruses, it is often used as a marker in diagnostic immunoassays in the ELISA and colloidal gold-based rapid test kits. However, positive results may be due to past or present infection with non-SARS-CoV-2 coronavirus strains, such as coronavirus HKU1, NL63, OC43, or 229E.
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