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Home > Molecular Biology > PCR > PCR Master Mixes
Convenient premix reagents needed for various PCRs. Except DNA template and primers, the PCR master mix contains all other components for PCR, including Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR stabilizer and enhancer at optimal concentrations for consistent and efficient PCR amplification.
    7 PCR Master Mixes Products
  • MB049-EQ2G: 2x Rapid PCR Master Mix, with green dye

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
    * Fast amplification speed: normally ~15 sec/kb; ~1 sec/kb if the amplification length is <1 kb.
    * High stability: repeated freezing and thawing for 50 times without significant decrease in activity.
    * Low cost: $400 for 50 ml 2x rapid PCR PreMix with dye. The cheapest PCR master mix for genotyping in the market.

  • MB067-EQ2G / MB067-EQ2B / MB067-EQ2R: 2x PCR PreMix, with dye (green, blue, or red)

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

    Features of the Taq DNA polymerases contained in the PCR master mix:
    * High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Terminal transferase activity of adding a single nucleotide (adenosine) at 3'-end of the extension product, facilitating TA cloning of PCR products.

  • MB040-HY2: 2x HY PCR Master Mix, with blue dye

    * The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
    * The high yield PCR Master Mix has increased amplification robustness, fidelity, yield, and fragment length, as well as the ability to handle difficult or “dirty” templates.
    * The tracking dye and precipitant have been added into the PCR PreMix so that the PCR product can be directly loaded for electrophoresis.

    Features of the blend of Taq enzyme and a proofreading enzyme contained in the high yield PCR master mix:
    * Robust processivity. Up to 10 kb human genomic and 15 kb lamda DNA fragments have been tested for amplification.
    * High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis. No contamination detected in standard PCR test reactions.
    * Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 3 months to amplify a single-copy gene from human genome with high efficiency.
    * Proofreading with 5′ exonuclease activity.

  • MB119-B: 2x hot-start high-fidelity PCR Master Mix, with blue dye
    * Simple operation: The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors. The tracking dye and precipitant have been added into the master mix so that the PCR product can be directly loaded for electrophoresis.
    * Ultra-high fidelity: The fidelity is 53 times that of Taq DNA Polymerase and 6 times that of Pfu DNA Polymerase. For every 300,000 bases amplified, there are fewer than 5 mismatches.
    * Fast amplification: normally 4-5 sec/kb; ~0.5 sec/kb if the amplification length is <1 kb; 4-150 times that of conventional PCR master mix.
    * Long fragment amplification: up to 40 kb from simple templates such as λDNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
    * Wide adaptability: It is suitable for the amplification of various GC content fragments, has super tolerance to PCR inhibitors, and can be used for direct PCR of a variety of samples.
    * Features of the hot-start high fidelity DNA polymerase (catalog No. MB118) contained in the 2x hot-start high-fidelity PCR master mix.

  • MB073-PCR: 2x Long Taq PCR MasterMix (With Dye)

    For reaction set-up, all you have to do is to add templates, specific primers, and water.

  • MB103-11: Cell and Tissue Direct PCR Kit (rapid genomic DNA extraction coupled with PCR)

    Solution based single tube protocol for rapid genomic DNA extraction;
    Extract genomic DNA from animal cells and tissue samples such as mouse tail and cultured cells in <10 minutes;
    No toxic chemicals, DNA precipitation and column purification, or proteinase digestion;
    Time saving, convenient, consistent, and minimal risk of contamination and pipetting errors;
    The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

  • MB103-21: Blood Direct PCR Kit (rapid genomic DNA extraction coupled with PCR)

    Solution based single tube protocol for rapid genomic DNA extraction;
    Extract genomic DNA from fresh or anti-coagulated blood in <10 minutes;
    No toxic chemicals, DNA precipitation and column purification, or proteinase digestion;
    Time saving, convenient, consistent, and minimal risk of contamination and pipetting errors;
    The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.

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