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Mouse IgG1 Isotype Control Antibody

Recombinant Mouse IgG1 Isotype Control, Mouse IgG1 Negative Control Antibody, mIgG1 Isotype Control

Catalog No. Product Name Size List Price (US$) Quantity
PA007126 In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody 1 mg 295.00
PA007126 In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody 5 mg 995.00
PA007126 In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody 25 mg 2950.00
PA007135 In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody 1 mg 295.00
PA007135 In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody 5 mg 995.00
PA007135 In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody 25 mg 2950.00
Description

Mouse IgG1 isotype control and mutants (natural and engineered mIgG1 isotype controls) are useful for in vitro and in vivo studies from ELISA, Western blot (WB), to cell-based assay to animal model study. The isotypes of mouse IgG antibody reagents used in scientific research and clinical diagnostics are mainly mouse IgG1 kappa. Mouse IgG1 antibodies and engineered mutants are also used in therapeutic drug discovery. Mouse IgG1 antibodies are more like human IgG4 antibodies than human IgG1 antibodies, which behave more like mouse IgG2a or IgG2c antibodies. Many antibody drugs especially those targetting immune checkpoint proteins use the human IgG4 constant regions. 

Please contact us to ask for a quote for the Fc silenced mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG1 isotype control and Fc-slient mutants are available.

PA007126: In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody (mIgG1 Isotype Control)

The in vivo grade recombinant mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in CHO cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for mouse IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of PBS, pH 7.0.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007135: In Vivo Grade Recombinant Mouse IgG1-D265A Isotype Control Antibody (mIgG1 D265A Isotype Control)

The in vivo grade recombinant mouse IgG1-D265A isotype control antibody (mIgG1 D265A isotype control) was produced in mammalian cells.
Immunogen: N/A.
Clone: 12B9.
Isotype: mouse IgG1, kappa.
Applications: an isotype-matched negative control for mouse IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Formulation: 0.2 μM filtered solution of PBS, pH 7.0.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

Shipping: The recombinant mouse IgG1 isotype control and mutants are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Mouse IgG antibodies are classified to four subclasses mainly based on the isotype of the heavy chain (IgG1, IgG2a or IgG2c, IgG2b, and IgG3 for the heavy chain; kappa and lamda for the light chain), of which IgG1 and kappa are the most abundantnamed in serum. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains, two identical heavy chains of about 50 kDa and two identical light chains of about 25 kDa. The four peptides are arranged in a Y-shape tetramer by disulfide bonds formed between the two heavy chains and between a heavy chain and a light chain.

Questions and Answers:

Question: MOPC21 or MOPC-21 (hybridoma clone number, isotype: mouse IgG1, kappa) is widely used as a mouse IgG1 isotype control produced directly by hybridoma or in the recombinant formats. Is your mouse IgG1 isotype control also Mouse IgG1, kappa monoclonal (MOPC-21) - isotype control?
Answer: Similar to mouse IgG1 isotype control (MOPC-21), no matter purified from hybridoma or produce by the recombinant method, our recombinant mouse IgG1 isotype controls (catalog # PA007126 and PA007135) has unknown binding specificity. But our mouse IgG1 isotype controls are not from the MOPC-21 hybridoma or its antibody sequences though we also produce and sell the recombinant mouse (MOPC-21) mAb IgG1 isotype control as well as Fc mutants. Please contact us and ask for the clone MOPC-21 derived recombinant mouse IgG1-k isotype controls if you are interested.

Question: What is the target of your mouse IgG1 kappa isotype control antibody?
Answer: Similar to our other mouse IgG isotype control antibodies, the mouse IgG1 isotype control antibody (catalog # PA007126) does not bind to any protein or chemical, even to those popular antigens for isotype control antibodies such as KLH, TNP, DNP, GST, Hen Egg Lysozyme (HEL), His-tag, Flag, c-myc, HA, and GFP. PA007126 is an engineered mouse IgG1 kappa antibody without antigen binding activity. We also produce and sell recombinant mouse IgG1 kappa isotype controls and Fc mutants against KLH, TNP, DNP,  GST, HEL, His-tag, Flag, c-myc, HA, and GFP. Please contact us and ask for them if you are interested.

Question: Is PA007126 your prefered mouse IgG1 kappa isotype control. Correct?
Answer: Yes because it was engineered to lose the target binding activity. Some clients are concerned about the possibility that it may bind another target after the binding to one target becomes weak. But so far, we have not found that the mouse IgG1 isotype control binds to any other target. In addition, its production yield is higher so that we can reduce the production cost and our selling prices. 

Question: What is the advantage of the recombinant antibody production method compared to the hybridoma method?
Answer: We use chemically defined medium for cell culturing to produce the recombinant antibody. There is significant advantage of the in vitro method. First, we do not use mouse ascites for antibody production. Second, although antibodies can be produced by cell culturing from over 90% of hybridomas, there is a finite and significant failure rate. The recombinant method can be of help. Third, some companies and scientists add FBS into hybridoma cell culturing medium, which results in antibody contamination. There is no such contamination in production of recombinant antibodies with the chemically defined medium. Fourth, we can easily switch the isotype with different antibody constant regions, and generate mutations such as D265A which is used in therapeutic antibody research. It is better to use the same isotype control mutation as the primary antibody in the application. 

Question: What is an isotype control antibody and why is it used?
Answer: An ideal isotype control matches the isotype of the primary antibody used in the application but lacks binding to the target. It is normally used as a negative control to differentiate, if there is any, non-specific background signal from the specific antibody signal. Mouse IgG1 kappa isotype controls against KLH, TNP, DNP,  GST, HEL, His-tag, Flag, c-myc, HA, and GFP meet the requirements in some cases but do not in specific cases in the presence of the targets or cross-reactivity.

Other in vivo grade Recombinant IgG Isotype Control Antibodies and Mutants:
In vivo Grade Recombinant Human IgG1 Isotype Control Antibody and Mutants
In vivo Grade Recombinant Human IgG2 Isotype Control Antibody
In vivo Grade Recombinant Human IgG4-S228P Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2a Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2b Isotype Control Antibody and Mutants
In vivo Grade Recombinant Mouse IgG2c Isotype Control Antibody and Mutants
In vivo Grade Recombinant Rat IgG1 Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2a Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2b Isotype Control Antibody
In vivo Grade Recombinant Rat IgG2c Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG1 Isotype Control Antibody
In vivo Grade Recombinant Hamster IgG2 Isotype Control Antibody

In vivo Grade Recombinant IgG Fc Proteins:
In vivo Grade Recombinant Human Fc Protein (hIgG1)
In vivo Grade Recombinant Human Fc Protein (hIgG2)
In vivo Grade Recombinant Human Fc Protein (hIgG4)
In vivo Grade Recombinant Mouse Fc Protein (mIgG1)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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