Single B cell antibody cloning and sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by next generation sequencing (NGS) including nanopore sequencing has been successfully applied to single B cell antibody technologies.
20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
BS046B:
Gene synthesis of the variable regions of the recombinant IgG antibodies;
Molecular construction of the recombinant IgG antibodies;
0.04 Liter transient production of the recombinant IgG antibodies in CHO cells;
Protein A purification.BS079B:
Gene synthesis of the variable regions of the recombinant scFv-Fc antibodies;
Molecular construction of the recombinant scFv-Fc antibodies;
0.04 Liter transient production of the recombinant scFv-Fc antibodies in CHO cells;
Protein A purification.The most comprehensive rat monoclonal antibody development services: custom production of rat monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.
The highest quality standard in the field of rat monoclonal antibody development services: guaranteed ELISA > 1:40,000.
Choice for licensing antibodies to save your cost and even make money.
Fast turnaround: 24 weeks once the antigen is available.The most comprehensive hybridoma-based mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins as well as small molecular compounds; for ELISA, Western blot, IHC, and flow cytometry applications.
The lowest custom mouse monoclonal antibody production price: $6000.
Fast turnaround: 24 weeks once the antigen is available.Custom Ilama and alpaca monoclonal antibody development (VHH, sdAb or nanobody) by Ilama or alpaca immunization and phage display screening.
Production of camel, Ilama or alpaca VHH in E.coli or VHH-Fc in HEK 293 cells.
Fast turnaround: 4-6 months once the antigen is available.The most comprehensive murine hybridoma generation for therapeutic antibody services:
Genetic background: white, black, immunodeficiency, knock-out, and humanized mice; rat; hamster;
Antigens: proteins, peptides, DNA, cells expressing certain proteins.
All are done in US.
The most comprehensive rabbit polyclonal antibody production services with the gold standard of rabbit immunization service (1 immunization and 4 boosts): custom production of rabbit polyclonal antibodies (pAb) against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; rabbit polyclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.
The highest quality standard in the field of rabbit polyclonal antibody production services: guaranteed ELISA > 1:64,000 (antisera) and > 1: 128,000 (purified antibodies).
Fast turnaround: 10 weeks once the antigen is available.The most comprehensive rabbit monoclonal antibody production services: custom production of rabbit monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; rabbit monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Syd Labs team has successfully developed a large number of high specificity and high affinity rabbit monoclonal antibodies against a wide range of difficult targets such as small molecules, antibody idiotype, and antigens non-immunogenic in murine, camel or Ilama.
Fast turnaround: 18 weeks once the antigen is available.