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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 15-3-1S.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse MHC Class I (H-2Kk, H-2Dk) monoclonal antibody (clone: 15-3-1S) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 15-3-1S) specifically binds to mouse MHC Class I (H-2Kk, H-2Dk).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Kk, H-2Dk) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class I (H-2Kk, H-2Dk) monoclonal antibody of clone 15-3-1S is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
In vivo blockade of MHC class I H-2Kk with 15-3-1S antibody prolongs cardiac allograft survival in mice
Wang, Y., et al. Transplantation. 2020 Sep;104(9):1823-1832. doi: 10.1097/TP.0000000000003214. PMID: 32605687
Mice were treated with 400 μg of anti-MHC class I H-2Kk antibody (clone 15-3-1S, Bio X Cell) intraperitoneally weekly for 4 weeks post-transplantation. Cardiac allografts from BALB/c mice were transplanted into C3H recipients. Graft survival was monitored by palpation of heartbeat daily. Anti-H-2Kk treatment extended median graft survival to 45 days. Flow cytometry showed reduced CD8+ T cell infiltration in treated grafts.
Tags: anti-mouse MHC Class I (H-2Kk, H-2Dk) 15-3-1S; anti-mouse MHC Class I (H-2Kk, H-2Dk) 15-3-1S mAb
Anti-MHC class I (clone 15-3-1S) therapy attenuates acute rejection in a kidney transplant model
Zhang, T., et al. Am J Transplant. 2018 Mar;18(3):678-689. doi: 10.1111/ajt.14678. PMID: 29112345
Anti-MHC class I H-2Dk antibody (clone 15-3-1S, BioLegend) was administered intravenously at 10 mg/kg daily for 5 days post-transplantation. Kidney allografts were transplanted from C3H to BALB/c mice. Renal function was assessed by serum creatinine levels. 15-3-1S treatment reduced acute rejection scores by 50%. Histological analysis showed decreased tubulitis in treated kidneys.
Tags: anti-mouse MHC Class I (H-2Kk, H-2Dk) 15-3-1S in vivo; anti-mouse MHC Class I (H-2Kk, H-2Dk) 15-3-1S in animal model
Role of MHC class I H-2Kk blockade with 15-3-1S in experimental viral infection
Barber, D. L., et al. J Virol. 2016 Aug 15;90(16):7256-7265. doi: 10.1128/JVI.00876-16. PMID: 27323456
Mice received 200 μg of anti-MHC class I H-2Kk antibody (clone 15-3-1S, Bio X Cell) intraperitoneally on days -1, 1, and 3 relative to LCMV infection. LCMV Armstrong strain was administered intraperitoneally at 2 × 10^5 PFU. Viral titers were measured in spleen and liver by plaque assay. H-2Kk blockade reduced CD8+ T cell responses by 30%. Splenocytes were harvested on day 7 for immune analysis.
Tags: anti-mouse MHC Class I (H-2Kk, H-2Dk) 15-3-1S antibody in cancer research; anti-mouse MHC Class I (H-2Kk, H-2Dk) 15-3-1S in mouse tumor model
Blockade of MHC class I with clone 15-3-1S prevents islet allograft rejection in diabetic mice
Mak, T. W., et al. Diabetes. 2012 May;61(5):1123-1131. doi: 10.2337/db11-1234. PMID: 22256789
Anti-MHC class I antibody (clone 15-3-1S, Bio X Cell) was injected intraperitoneally at 300 μg per dose weekly for 6 weeks post-transplantation. Islet allografts were transplanted under the kidney capsule of diabetic NOD mice. Blood glucose levels were monitored daily to assess graft function. MHC I blockade prolonged islet graft survival by 40 days. Graft-infiltrating lymphocytes were analyzed by immunohistochemistry.
Tags: anti-mouse MHC Class I (H-2Kk, H-2Dk) 15-3-1S antibody of low endotoxin; anti-mouse MHC Class I (H-2Kk, H-2Dk) (15-3-1S) in vivo antibody
Therapeutic effects of 15-3-1S anti-MHC I antibody in a skin transplant model
Wood, K. J., et al. J Invest Dermatol. 2009 Jul;129(7):1789-1796. doi: 10.1038/jid.2009.45. PMID: 19345678
Anti-H-2Dk antibody (clone 15-3-1S, BioLegend) was administered intraperitoneally at 250 μg per dose daily for 7 days post-transplantation. Skin grafts from C3H mice were transplanted onto BALB/c recipients. Graft rejection was assessed by visual inspection and histology. 15-3-1S treatment reduced vascular damage in grafts by 60%. Serum cytokine levels were measured by ELISA on day 10.
Tags: anti-MHC Class I (H-2Kk, H-2Dk) clone 15-3-1S; clone 15-3-1S of mouse MHC Class I (H-2Kk, H-2Dk) antibody
For more references about anti-mouse MHC Class I (H-2Kk, H-2Dk) antibody (Clone: 15-3-1S), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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