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Home > Antibodies > Antibody Production and Purification > Antibody Purification > Protein A

Protein A

Recombinant Protein A

Catalog No. Product Name Size List Price (US$) Quantity
IA019-10 Alkaline-Stabilized Recombinant Protein A 10 mg 150.00
IA019-500 Alkaline-Stabilized Recombinant Protein A 500 mg 900.00
IA019-1g Alkaline-Stabilized Recombinant Protein A 1 g 1500.00
IA019-10g Alkaline-Stabilized Recombinant Protein A 10 g 12000.00

Protein A binds to many immunoglobulins with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, and with moderate affinity to human IgM, IgA, and IgE as well as mouse IgG1 and IgG3. Protein A binds to the Fc region through interaction with the heavy chain.

Alkaline-stabilized recombinant protein A has been used in the most popular commercial products of protein A resins and pre-packed protein A columns with Clean in Place: 0.1-0.5 M NaOH. The recombinant protein A ligand also demonstrates improved target protein binding in many cases compared to the native protein A.

IA019: Alkaline-Stabilized Recombinant Protein A

Appearance (liquid): Clear, pale yellow with no particulates
Endotoxin < 1.0 EU/mg
Purity: > 95% (SDS-PAGE), > 95% (SEC-HPLC)
hIgG Binding: 100%
Binding Capacity: 50 - 80 mg/ml hIgH
Animal Free: Yes
Entertoxin B Risk: No
Fermentation Organism: E. coli

A variety of pre-packed protein A affinity columns are available with NMab protein A resin, NMab Pro protein A resin, and UniMab 50 HC Protein A resin in a range of flexible column diameters and bed heights. The alkaline-stabilized recombinant protein A can be detected by our Protein A ELISA kit for detection of natural and recombinant Protein A.

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