De novo antibody sequencing to obtain the intact amino acid sequence of variable and/or constant regions of monoclonal antibodies by LC-MS/MS.
Sample requirement: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/mL).
Fast turnaround time: 2-3 months per antibody, but may require more time depending on the complexity of analysis.Our FcRγ binding assay and FcRn binding assay services include: his-tagged receptors are immobilized onto anti-His biosensors; the loaded biosensors are dipped into a dilution series of the antibody; the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated.
Our epitope binning service include: Antigen is immobilized onto a biolayer interferometry (BLI) sensor using an appropriate method. Antibody 1 is allowed to saturate its epitope on the antigen. Binding of Antibody 2 to antigen saturated with Antibody 1 is assessed.
Fast turnaround: 2-3 weeks.
Denaturation and reduction followed by LC-MS (liquid chromatography-mass spectrometry) analysis.
Fast turnaround time: 1- 2 weeks.
Sample requirement: >100 ug at 1 mg/ml.Peptide mapping by LC-MS/MS can be used to determine detailed information about the peptide's sequence and post-translational modifications.
- Ion-Exchange HPLC (IEX-HPLC) for separation of samples based on differences in their surface charge.
Sample requirement: 100-200 ug.
Fast Turnaround time: 1 - 2 weeks. - Normal-phase chromatography (NP-HPLC) is an analytical technique that is used to separate molecules on the basis of differences in the strength of their interaction with a polar stationary phase.
Sample requirement: 100-200 ug.
Fast turnaround time: 2 - 3 weeks. - Reversed-phase chromatography (RP-HPLC) is an analytical technique that separates molecules on the basis of differences in their hydrophobicity.
Sample requirement: 50-100 ug.
Fast turnaround time: 1 - 2 weeks. Capillary electrophoresis (CE) analysis using Caliper LC90.
Sample requirement: 50-100 ug.
Fast turnaround time: 1 - 2 weeks.Capillary isoelectric focusing is an analytical technique for characterization of protein mixtures and determination of protein isoelectric points.
Sample requirement: 50-100 ug.
Fast turnaround time: 1 - 2 weeks.