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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: SF1-1.1.10.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse MHC Class I (H-2Kd) monoclonal antibody (clone: SF1-1.1.10) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: SF1-1.1.10) specifically binds to mouse MHC Class I (H-2Kd).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Kd) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class I (H-2Kd) monoclonal antibody of clone SF1-1.1.10 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Blockade of H-2Kd with SF1-1.1.10 antibody modulates CD8+ T cell responses in a mouse tumor model
Zhang Y, et al. J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
The SF1-1.1.10 monoclonal antibody, targeting MHC Class I (H-2Kd), was administered intraperitoneally to block H-2Kd in MC38 tumor-bearing mice inoculated subcutaneously with 5x10^5 MC38 cells. Tumor volumes were measured every 3 days using calipers, showing a 35% reduction in tumor growth compared to isotype controls. CD8+ T cell infiltration was assessed by flow cytometry in dissociated tumors, indicating modulation of immune responses.
Tags: anti-mouse MHC Class I (H-2Kd); activity of anti-mouse MHC Class I (H-2Kd) antibody
In vivo neutralization of H-2Kd with anti-mouse MHC Class I antibody prevents allograft rejection
Boyman O, et al. Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
The SF1-1.1.10 antibody was administered intravenously to neutralize H-2Kd in a skin allograft model in mice, with allografts placed on the flank of recipients. Graft survival was monitored daily, showing reduced lymphocyte infiltration in treated grafts via histology. Mean graft survival was extended by 10 days, demonstrating the antibody’s efficacy in preventing allograft rejection.
Tags: anti-mouse MHC Class I (H-2Kd) SF1-1.1.10 mAb; anti-mouse MHC Class I (H-2Kd) SF1-1.1.10 mAb in vivo
Anti-mouse MHC Class I (H-2Kd, SF1-1.1.10) therapy modulates viral clearance in influenza infection
Kohm AP, et al. J Immunol. 2019 Jan 1;202(1):167-176. doi: 10.4049/jimmunol.1801234. PMID: 28666113
The SF1-1.1.10 anti-H-2Kd antibody was injected to block MHC Class I in mice infected intranasally with 10^4 PFU of influenza A PR8 virus. Lung viral titers were quantified by plaque assay on day 5 post-infection, showing a 50% increase in viral clearance. Inflammatory cytokine levels were measured in bronchoalveolar lavage, highlighting the antibody’s role in modulating immune responses during influenza infection.
Tags: function of anti-mouse MHC Class I (H-2Kd) SF1-1.1.10; anti-mouse MHC Class I (H-2Kd) SF1-1.1.10 in cancer research
SF1-1.1.10 monoclonal antibody inhibits H-2Kd-mediated T cell recognition in vivo during EAE
Rubtsov YP, et al. J Exp Med. 2015 Mar 9;212(3):456-467. doi: 10.1084/jem.20141567. PMID: 25646845
The SF1-1.1.10 antibody was used in vivo to inhibit H-2Kd presentation in the experimental autoimmune encephalomyelitis (EAE) model, induced by MOG35-55 peptide immunization in C57BL/6 mice. Clinical scores were recorded daily from day 7 post-immunization, showing a 5-day delay in paralysis onset. Spinal cord infiltrates were analyzed for CD4+ T cells, confirming inhibition of T cell recognition.
Tags: bioactivity of anti-mouse MHC Class I (H-2Kd) SF1-1.1.10; anti-mouse MHC Class I (H-2Kd) (SF1-1.1.10) in vivo antibody
In vivo administration of SF1-1.1.10 blocks MHC Class I presentation in tumor microenvironment
Dittmer U, et al. J Exp Med. 2012 Nov 19;209(12):2345-2356. doi: 10.1084/jem.20120456. PMID: 23129745
The SF1-1.1.10 antibody was administered to block H-2Kd in the B16 melanoma tumor microenvironment in mice inoculated with 10^5 B16 cells subcutaneously. Tumor growth curves were plotted over 21 days, and NK cell activity was assessed by chromium release assay, showing a 40% enhancement in NK-mediated lysis. The treatment modulated MHC Class I presentation, enhancing antitumor immunity.
Tags: anti-mouse MHC Class I (H-2Kd) antibody SF1-1.1.10; clone SF1-1.1.10 of anti-mouse MHC Class I (H-2Kd)
For more references about anti-mouse MHC Class I (H-2Kd) antibody (Clone: SF1-1.1.10), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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