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Home > Proteins & Peptides > A-Z Proteins  > Biotinylated Recombinant SARS-CoV-2 Spike S1 Protein

Biotinylated Recombinant SARS-CoV-2 Spike S1 Protein

2019-nCoV S Protein S1 Subunit, COVID-19 Virus Spike Protein S1 Subunit

Catalog No. Product Name Size List Price (US$) Quantity
BP003055 Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein 50 ug 380.00
BP003055 Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein 400 ug 2400.00
BP003064 Biotinylated Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein 50 ug 380.00
BP003064 Biotinylated Recombinant SARS-CoV-2 Spike S1-Fc-Avi Protein 400 ug 2400.00
BP003072 Biotinylated Recombinant SARS Spike S1-His-Avi Protein 50 ug 380.00
BP003072 Biotinylated Recombinant SARS Spike S1-His-Avi Protein 400 ug 2400.00
BP003074 Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein 50 ug 380.00
BP003074 Biotinylated Recombinant SARS Spike S1-Fc-Avi Protein 400 ug 2400.00
Description

BP003055: Biotinylated Recombinant SARS-CoV-2 Spike S1-His-Avi Protein (Spike Protein S1 Subunit)

Source: recombinant SARS-CoV-2 Spike S1 protein (S1 subunit, Gln14-Arg683) was expressed in mammalian cells with a His tag and Avi at the C-terminus.
Accession: QHD43416.1
Predicted molecular mass: 77.9 kDa. Due to glycosylation, the biotinylated recombinant SARS-CoV-2 Spike S1 protein migrates to 110-120 kDa based on the Bis-Tris PAGE result.
Endotoxin: Less than 1 EU per ug by the LAL method.
Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein at 1 ug/ml (100 ul/well). Dose response curve for the recombinant human ACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA.
Formulation: The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.

Shipping: The biotinylated recombinant SARS-CoV-2 Spike S1-His-Avi protein is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution.

Background

The spike protein (S protein) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on the S protein mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

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