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Conditions of optimal DMT-2'-F-dU Phosphoramidite performance should be determined experimentally by the investigator. Its precursors, 2'-F-dU (CAS # 784-71-4) and 2'-F-5'-O-DMT-2'-dU (CAS # 146954-74-7), are available.
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APIM050503: DMT-2'-F-dU Phosphoramidite
CAS No.: 146954-75-8.
Molecular Formula: C39H46FN4O8P.
Molecular Weight: 748.8.
Appearance: (visual inspection) white to off-white powder.
Identity: (molecular weight by MS) conforms to structure molecular weight; (1H NMR) conforms to structure.
Purity: (31P NMR) ≥99.0%; (RP-HPLC) ≥99.0%.
Impurity profile: (HPLC) any single critical impurity ≤ 0.15%; total critical impurities ≤ 0.3%; total impurities ≤ 1.0%.
Assay: (HPLC) 98.0%~102.0%.
Sulfate ash: (muffle furnace) ≤0.1%.
Residual solven: (GC) ≤1.5%.
Moisture content: (Karl Fischer) ≤0.2%
Clarity of solution: (visual inspection) the solution shall be clear and free of insoluble substances.
Storage: -20±5℃.
2-Fluoro-Phosphoramidites:
DMT-2'-Fluoro-dA(Bz)-CE-Phosphoramidite (CAS No.: 136834-22-5)
DMT-2'-Fluoro-dG(iBu)-CE-Phosphoramidite (CAS No.: 144089-97-4)
DMT-2'-Fluoro-Ac-dC-CE-Phosphoramidite (CAS No.: 159414-99-0)
DMT-2'-Fluoro-dU Phosphoramidite (CAS No.: 146954-75-8)
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