Syd Labs has extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized secretion signal and linker sequences, Syd Labs can efficiently complete a project from DNA construction and transient production of Fc fusion proteins in CHO and HEK 293 cells, to Fc fusion protein purification. N-term or C-term of Fc fusions of peptides and proteins in secreted form and multiple Fc backbone are available upon request.
BS050B:
Synthesis of the gene encoding the Fc fusion protein;
Molecular construction of the recombinant Fc fusion protein;
0.04 Liter transient production of the recombinant Fc fusion protein in CHO or HEK293 cells;
Protein A purification.BS052: HRP Conjugation Service
1. Highly efficient process to conjugate HRP to clients' proteins and antibodies
2. Amine-reactive HRP achieves the highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. HRP enzyme activity is ~150 units/mg
5. Fast turnaround time: 3-5 daysBS077: Fluorophore Conjugation Service
1. Highly efficient process to conjugate all commercially available fluorophore to clients' proteins and antibodies
2. The highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. Fast turnaround time: 3-5 daysBS076: Biotin Conjugation Service
1. Highly efficient process to conjugate biotin to clients' peptides, proteins or antibodies
2. The highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. Fast turnaround time: 3-5 daysBS054: Magnetic Bead Conjugation Service
Highly efficient process to conjugate magnetic beads to clients' peptides, proteins, and antibodies: the ratio is 20 ug protein or antibody per mg magnetic microbeads in the conjugation reaction; about 6 ug protein or antibody would be conjugated onto the beads at a concentration of 6 ug antibody per mg magnetic beads.
Fast turnaround time: 3-5 days.Free consulting services for selecting expression vectors.
Free consulting services for selecting protein fragments with higher immunogenicity and antigen specificity.Free consulting services for selecting expression vectors.
Free consulting services for selecting protein fragments with higher immunogenicity and antigen specificity.Free consulting services for selecting expression vectors.
Free consulting services for selecting protein fragments with higher immunogenicity and antigen specificity.The standard Western Blot protocol will be used to detect protein expression in solution, cell or tissue samples.
Free consulting services for selecting expression vectors.
Free consulting services for selecting protein fragments with higher immunogenicity and antigen specificity.