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Home > Antibodies > Anti-Mouse Antibodies (In Vivo) > Anti-mouse MHC Class II (I-A / I-E) Monoclonal Antibodies

Anti-mouse MHC Class II (I-A / I-E) Monoclonal Antibodies

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

Catalog No. Product Name Size List Price (US$) Quantity
PA007536.r2b In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone: M5/114.15.2), Rat IgG2b Kappa 1 mg 150.00
PA007536.r2b In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone: M5/114.15.2), Rat IgG2b Kappa 5 mg 350.00
PA007536.r2b In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone: M5/114.15.2), Rat IgG2b Kappa 25 mg 900.00
Description

PA007536.r2b: In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone:  M5/114.15.2), Rat IgG2b Kappa

Recombinant Rat IgG2b Monoclonal Antibody.
Clone: M5/114.15.2.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse MHC Class II (I-A/I-E) monoclonal antibody (clone: M5/114.15.2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M5/114.15.2) specifically binds to mouse MHC Class II (I-A/I-E).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (I-A/I-E) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.

Shipping: The in vivo grade recombinant anti-mouse MHC Class II (I-A/I-E) monoclonal antibody of clone M5/114.15.2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.

References of Anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone: M5/114.15.2)

Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells.
Heiner von Buttlar, et al. PLoS One. 2014 Aug 29;9(8):e106188. PMID: 25171368
For surface marker staining the following antibodies were used: anti-mouse CD11c (clone N418); anti-mouse CD11b (clone M1/70); anti-mouse B220 (clone RA3-6B2); anti-mouse CD86 (clone GL1); anti-mouse MHC-II I-A/I-E (clone M5/114.15.2) and respective isotype controls (all: ebioscience, Frankfurt, Germany).
(Tags: anti-mouse MHC Class II (I-A/I-E); activity of anti-mouse MHC Class II (I-A/I-E) antibody)
Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells.
Shuang Geng, et al. BMC Immunol. 2011 May 5:12:27. PMID: 21542943
Using either clonotypic CD4+ T cells from DO11.10 Balb/c mice or polyclonal CD4+ T cells from ovalbumin-sensitized Balb/c mice, we found that the induction of CD25- iTreg in either case could be blocked by anti-MHC-II antibody and, therefore, was MHC-II-dependent. Thus, antigenic stimulation is essential for the induction of CD25- iTreg...
(Tags: anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 mAb; anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 mAb in vivo)
An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens.
Bei Wang, et al. Vaccine. 2006 Apr 24;24(17):3477-89. PMID: 16530303
The antibodies used for blocking were: mouse IgG1 isotype antibody, rat anti-mouse CD4 monoclonal antibody, rat anti-mouse CD8a monoclonal antibody, mouse anti-mouse H-2Dd monoclonal antibody, mouse anti-mouse H-2Kd monoclonal antibody (BD, Pharmingen), rat anti-mouse MHC class II (I-A/I-E) (M5/114.15.2), or rat anti-mouse NKG2D monoclonal antibody (R&D system, Inc. Minneapolis, MN).
(Tags: function of anti-mouse MHC Class II (I-A/I-E) M5/114.15.2; anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 in cancer research)
For more technical references or data sheets regarding the Recombinant Anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody (Clone: M5/114.15.2) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry

Related Links
Anti-mouse/rat MHC Class II (I-Ek/RT1-D) Monoclonal Antibody (Clone: 14-4-4S)
Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277)
Anti-mouse MHC Class II (I-A) Monoclonal Antibody (Clone: Y-3P)
Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibody (Clone: 10-3.6.2)
Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibody (Clone: AF6-88.5.3)
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