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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: M5/114.15.2.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse MHC Class II (I-A/I-E) monoclonal antibody (clone: M5/114.15.2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M5/114.15.2) specifically binds to mouse MHC Class II (I-A/I-E).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (I-A/I-E) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class II (I-A/I-E) monoclonal antibody of clone M5/114.15.2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
MHC Class II Presentation Is Affected by Polymorphism in the H2-Ob Gene and Additional Loci
Glanville J, et al. Front Immunol. 2021 Jun 29;12:683374. doi: 10.3389/fimmu.2021.683374. PMID: 34268064
N2 mice were analyzed in groups (~10?20 mice per group) at 8 weeks of age along with age-matched B6J mice (3?5 mice per group) and their splenic B cells, identified as CD19+ were stained with 15G4 (MHCII-CLIP) or M5/114 (total MHCII). Twelve weeks post-transplantation the levels of MHCII and MHCII-CLIP were measured on the surface of CD19+ peripheral blood B cells or splenic B cells from the chimeric mice. To normalize each sample’s MHCII-CLIP level to this sample’s level of MHCII, the gMFI obtained for 15G4 (MHCII-CLIP) was divided by the gMFI obtained for M5/114 (MHCII). H2-O, a MHC class II-like protein, sets a threshold for B-cell entry into germinal centers. TLR agonists downregulate H2-O in CD8alpha- dendritic cells.
Tags: anti-mouse MHC Class II (I-A/I-E); activity of anti-mouse MHC Class II (I-A/I-E) antibody
Murine cytomegalovirus degrades MHC class II to colonize the salivary glands
Nikolaou E, et al. Nat Microbiol. 2018 Apr;3(4):356-366. doi: 10.1038/s41564-018-0121-0. PMID: 29447285
They were then stained for MHC II with Alexa647-conjugated mAb M5/114 and analysed by flow cytometry. The table quantitates MHC II expression for GFP+ cells. RAW-C2TA cells were infected with MCMV-GFP (3 p.f.u. / cell, 2h). Then bafilomycin (20nM) was added or not (control), and the cells cultured for a further 6h or 24h. The MHC II rescued by bafilomycin had an endosomal distribution.
Tags: anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 mAb; anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 mAb in vivo
Mice lacking all conventional MHC class II genes
Madsen LS, et al. Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10338-43. doi: 10.1073/pnas.96.19.10338. PMID: 10468609
Upper and Lower panels present staining with Y3P or M5/114 anti-class-II antibodies, respectively (thin lines). Expression of MHC-II molecules in MHCII?/? mice. (A) Flow-cytometry profiles of anti-class-II staining on IgM+ splenocytes in MHCII+/? and MHCII?/? littermates. MHC class II (MHC-II) molecules play a central role in the selection of the T cell repertoire, in the establishment and regulation of the adaptive immune response, and in autoimmune deviation. We have generated knockout mice lacking all four of the classical murine MHC-II genes (MHCII(?/?) mice).
Tags: function of anti-mouse MHC Class II (I-A/I-E) M5/114.15.2; anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 in cancer research
Developmental dissociation of T cells from B, NK, and myeloid cells revealed by MHC class II-specific chimeric immune receptors bearing TCR-zeta or FcR-gamma chain signaling domains
Lin WY, et al. Blood. 2002 Oct 15;100(8):3045-8. doi: 10.1182/blood-2002-02-0428. PMID: 12070035
Chimeric receptors were expressed in bone marrow cells from MHC class II knockout mice, and these cells were used to reconstitute irradiated recipients. In vivo development of T cells was rescued by MHC class II-specific receptors bearing TCR-zeta signaling domains. MHC class II-specific receptors bearing FcR-gamma signaling domains directed development of B, NK, and myeloid cells, but not T cells. These results indicate that MHC class II-specific chimeric receptors can be used to dissect the developmental signals that are required for T-cell differentiation. Recipients of TCR-zeta-bearing receptors developed mature T cells that were able to reject allogeneic skin grafts.
Tags: bioactivity of anti-mouse MHC Class II (I-A/I-E) M5/114.15.2; anti-mouse MHC Class II (I-A/I-E) (M5/114.15.2) in vivo antibody
H2-O, a MHC class II-like protein, sets a threshold for B-cell entry into germinal centers
Draghi NA, et al. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16607-12. doi: 10.1073/pnas.1004664107. PMID: 20807742
H2-O-deficient B cells entered germinal centers more efficiently than wild-type B cells in vivo. H2-O expression was upregulated on germinal center B cells. In vivo, H2-O-deficient mice had larger germinal centers and higher antibody titers. H2-O sets a threshold for B-cell entry into germinal centers by modulating MHC class II peptide loading. Adoptive transfer experiments confirmed H2-O's role in B-cell selection in vivo.
Tags: anti-mouse MHC Class II (I-A/I-E) antibody M5/114.15.2; clone M5/114.15.2 of anti-mouse MHC Class II (I-A/I-E)
For more references about anti-mouse MHC Class II (I-A/I-E) antibody (Clone: M5/114.15.2), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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