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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Rat IgG2b Monoclonal Antibody.
Clone: M5/114.15.2.
Isotype: Rat IgG2b kappa.
Source: The anti-mouse MHC Class II (I-A/I-E) monoclonal antibody (clone: M5/114.15.2) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant rat monoclonal antibody (clone: M5/114.15.2) specifically binds to mouse MHC Class II (I-A/I-E).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (I-A/I-E) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class II (I-A/I-E) monoclonal antibody of clone M5/114.15.2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
1、Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells
Heiner von Buttlar, et al. PLoS One. 2014 Aug 29;9(8):e106188. doi: 10.1371/journal.pone.0106188. eCollection 2014.PMID: 25171368
For surface marker staining the following antibodies were used: anti-mouse CD11c (clone N418); anti-mouse CD11b (clone M1/70); anti-mouse B220 (clone RA3-6B2); anti-mouse CD86 (clone GL1); anti-mouse MHC-II I-A/I-E (clone M5/114.15.2) and respective isotype controls (all: ebioscience, Frankfurt, Germany). I
Tags: anti-mouse MHC Class II (I-A/I-E); activity of anti-mouse MHC Class II (I-A/I-E) antibody
2、Efficient induction of CD25- iTreg by co-immunization requires strongly antigenic epitopes for T cells
Shuang Geng, et al. BMC Immunol. 2011 May 5:12:27. doi: 10.1186/1471-2172-12-27. PMID: 21542943
Using either clonotypic CD4+ T cells from DO11.10 Balb/c mice or polyclonal CD4+ T cells from ovalbumin-sensitized Balb/c mice, we found that the induction of CD25- iTreg in either case could be blocked by anti-MHC-II antibody and, therefore, was MHC-II-dependent. Thus, antigenic stimulation is essential for the induction of CD25- iTreg...
Tags: anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 mAb; anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 mAb in vivo
3、An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens
Bei Wang, et al. Vaccine. 2006 Apr 24;24(17):3477-89. doi: 10.1016/j.vaccine.2006.02.025. Epub 2006 Feb 28. PMID: 16530303
The antibodies used for blocking were: mouse IgG1 isotype antibody, rat anti-mouse CD4 monoclonal antibody, rat anti-mouse CD8a monoclonal antibody, mouse anti-mouse H-2Dd monoclonal antibody, mouse anti-mouse H-2Kd monoclonal antibody (BD, Pharmingen), rat anti-mouse MHC class II (I-A/I-E) (M5/114.15.2), or rat anti-mouse NKG2D monoclonal antibody (R&D system, Inc. Minneapolis, MN).Tags: function of anti-mouse MHC Class II (I-A/I-E) M5/114.15.2; anti-mouse MHC Class II (I-A/I-E) M5/114.15.2 in cancer research
For more references about anti-mouse MHC Class II (I-A/I-E) antibody (Clone: M5/114.15.2) please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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