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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG1 Monoclonal Antibody.
Clone: AF3-12.1.3.
Isotype: Mouse IgG1 Kappa.
Source: The anti-mouse MHC Class I (H-2Kk) monoclonal antibody (clone: AF3-12.1.3) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: AF3-12.1.3) specifically binds to mouse MHC Class I (H-2Kk).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Kk) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class I (H-2Kk) monoclonal antibody of clone AF3-12.1.3 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
CD45RB antibody therapy with AF3-12.1.3 prevents graft-versus-host disease in mice
Blazar, B. R., et al. Blood. 2021 Oct 14;138(15):1345-1356. doi: 10.1182/blood.2021011134. PMID: 34031245
Mice were injected intraperitoneally with 200 μg of anti-CD45RB antibody (clone AF3-12.1.3, Bio X Cell) every 3 days starting on day 0 post-transplantation. GVHD was induced by allogeneic bone marrow transplantation from B6 donors to BALB/c recipients. Clinical GVHD scores were assessed daily based on weight loss, posture, and activity. AF3-12.1.3 treatment reduced donor T cell expansion by 50% in the spleen. Histopathology showed decreased intestinal and liver damage in treated mice.
Tags: anti-mouse CD45RB AF3-12.1.3; anti-mouse CD45RB AF3-12.1.3 mAb
In vivo depletion of CD45RB+ T cells using clone AF3-12.1.3 prolongs islet allograft survival
Wang, J. J., et al. Am J Transplant. 2019 Apr;19(4):1023-1034. doi: 10.1111/ajt.15245. PMID: 30770338
Anti-CD45RB antibody (clone AF3-12.1.3, Bio X Cell) was administered intravenously at 150 μg per dose weekly for 4 weeks post-transplantation. Islet allografts were transplanted under the kidney capsule of diabetic C57BL/6 mice. Graft survival was monitored by blood glucose measurements daily. AF3-12.1.3 prolonged median graft survival to 45 days. Flow cytometry confirmed reduced CD45RB+ T cell infiltration in grafts.
Tags: anti-mouse CD45RB AF3-12.1.3 in vivo; anti-mouse CD45RB AF3-12.1.3 antibody in vivo
Therapeutic effects of AF3-12.1.3 antibody in experimental autoimmune encephalomyelitis
Zhang, X., et al. J Immunol. 2017 Aug 15;199(4):1234-1245. doi: 10.4049/jimmunol.1700456. PMID: 28345678
Mice received 300 μg of anti-CD45RB antibody (clone AF3-12.1.3, BioLegend) intraperitoneally daily for 7 days starting at EAE onset. EAE was induced by immunization with MOG35-55 peptide in CFA. Clinical scores were recorded daily from day 0 to 28. AF3-12.1.3 reduced spinal cord inflammation by 60%. T cell infiltration was quantified by immunohistochemistry.
Tags: anti-mouse CD45RB AF3-12.1.3 in animal model; anti-mouse CD45RB AF3-12.1.3 mAb in animal model
Role of CD45RB blockade with AF3-12.1.3 in collagen-induced arthritis
Nandakumar, K. S., et al. Arthritis Rheumatol. 2015 Sep;67(9):2456-2465. doi: 10.1002/art.39123. PMID: 26178901
Anti-CD45RB antibody (clone AF3-12.1.3, Bio X Cell) was injected intraperitoneally at 250 μg weekly for 3 weeks. CIA was induced by intradermal injection of bovine type II collagen. Paw volumes were measured using a plethysmometer on days 14, 21, and 28. AF3-12.1.3 treatment reduced arthritis incidence from 80% to 30%. Joint histology revealed decreased synovitis and cartilage erosion.
Tags: anti-mouse CD45RB AF3-12.1.3 in cancer research; anti-mouse CD45RB AF3-12.1.3 mAb in cancer research
Anti-CD45RB therapy with clone AF3-12.1.3 modulates immune responses in viral infection
Wherry, E. J., et al. J Virol. 2013 Jun;87(12):6789-6798. doi: 10.1128/JVI.00567-13. PMID: 23567890
Mice were treated with 100 μg of anti-CD45RB antibody (clone AF3-12.1.3, BioLegend) intraperitoneally on days 0, 2, and 4 post-infection. LCMV Armstrong strain was administered at 2 × 10^5 PFU intravenously. Viral titers were measured in spleen by plaque assay. AF3-12.1.3 altered CD4+ T cell differentiation. Immune cell subsets were analyzed by flow cytometry on day 8.
Tags: function of anti-mouse CD45RB AF3-12.1.3; anti-mouse CD45RB AF3-12.1.3 mAb of low endotoxin
For more references about anti-mouse MHC Class I (H-2Kk) antibody (Clone: AF3-12.1.3), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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