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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: Y-3P.
Isotype: Mouse IgG2a kappa.
Source: The anti-mouse MHC Class II (I-A) monoclonal antibody (clone: Y-3P) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: Y-3P) specifically binds to mouse MHC Class II (I-A) .
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (I-A) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class II (I-A) monoclonal antibody of clone Y-3P is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Tissue-specific expression of B7x protects from CD4 T cell-mediated autoimmunity
Wei, J., et al. J Exp Med. 2011 Aug 1;208(8):1683-94. doi: 10.1084/jem.20100639. PMID: 21727190
This lack of disease induction has been attributed to the loss of IFN-?R on ? cells and APCs such as macrophages. It has been postulated that the loss of IFN-? responsiveness inhibits the up-regulation of MHC class I and II molecules and also prevents efficient killing of ? cells as this cytokine has been shown to regulate apoptosis and iNOS (inducible nitric oxide synthetase) production. ...Although high expression of most B7 family members is largely restricted to immune cells, especially APCs, we were not able to detect protein expression of B7x in any cell type of hematopoietic origin. ...However, histological staining of pancreatic sections demonstrated constitutive expression of B7x in the islets of Langerhans, indicating that this inhibitory molecule may have a role in maintaining peripheral tolerance to islet-reactive T cells. ...It has already been shown that PD-L1, another member of the B7 family, plays an important role in preventing diabetes by inhibiting islet-reactive T cells. ...Furthermore, PD-L1 expression on islets results in protection from autoreactive T cells in diabetic mice in an islet transplantation model.
Tags: anti-mouse MHC Class II (I-A) Y-3P mAb in cancer research; anti-mouse MHC Class II (I-A) Y-3P mAb in mouse tumor model
Memory phenotype CD4 T cells undergoing rapid, nonburst-like, cytokine-driven proliferation can be distinguished from antigen-experienced memory cells
Younes, S. A., et al. PLoS Biol. 2011 Oct;9(10):e1001171. doi: 10.1371/journal.pbio.1001171. PMID: 22022231
One approach to evaluating the importance of antigen stimulation in T-cell dynamics is to determine whether proliferation can be blocked by anti-MHC class II antibodies. ...To that end, we utilized Fc?R??/? mice so that the anti-class II antibody would not block T-cell responses by elimination of antigen-presenting cells. ...In such mice, anti-class II antibodies powerfully inhibit antigen-specific in vivo responses. ...Neither anti?IL-15 nor anti?IL-7R? had any inhibitory effect, but Y3P almost completely inhibited proliferation, indicating that the expansion of cells in this lymphopenic setting required recognition of MHC and presumably of peptide/MHC complexes. ...Here we have shown that the in situ proliferation of MP cells is not inhibited by anti-MHC class II antibody, using a reagent that strikingly inhibits the proliferation of antigen-specific cells in response to antigen challenge and that blocks the rapid proliferation of MP cells transferred to lymphopenic recipients.
Tags: bioactivity of anti-mouse MHC Class II (I-A) Y-3P; anti-mouse MHC Class II (I-A) Y-3P of low endotoxin
For more references about Anti-mouse MHC Class II (I-A) Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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