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Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human HLA-DR/DP/DQ Mouse IgG1 Kappa monoclonal antibody was produced in mammalian cells.
Clone: F3.3.
Isotype: Mouse IgG1 Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: F3.3) specifically binds to human HLA-DR/DP/DQ.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human HLA-DR/DP/DQ protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human HLA-DR/DP/DQ antibodies (clone of F3.3) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
In vivo cytotoxicity of type I CD20 antibodies critically depends on Fc receptor ITAM signaling
de Haij, S., et al. (2010). Cancer Res. 2010 Apr 15;70(8):3209-17. doi: 10.1158/0008-5472.CAN-09-2869. PMID: 20354182
The F3.3 antibody (anti-human HLA-DR/DP/DQ) was used as a control for HLA class II binding in in vivo experiments, showing no significant cytotoxicity without ITAM signaling. In vivo depletion studies in mouse models confirmed that neutrophils armed with F3.3 bispecific constructs mediated tumor clearance, highlighting the critical role of Fc receptor signaling in antibody-mediated efficacy.
Tags: anti-human HLA-DR/DP/DQ F3.3; anti-human HLA-DR/DP/DQ F3.3 mAb
Chimeric IgA antibodies against HLA class II effectively trigger lymphoma cell killing
Dechant, M., et al. (2002). Blood. 2002 Dec 15;100(13):4574-80. doi: 10.1182/blood-2002-03-0687. PMID: 12393717
Chimeric F3.3 antibodies (anti-human HLA-DR/DP/DQ), including human IgA1, IgA2, IgG1, IgG2, IgG3, and IgG4 isotypes, were generated using variable regions from the F3.3 hybridoma (GenBank AY058910 [V L], AY058911 [V H]). Saturating concentrations showed similar binding to HLA class II-transfected L66 cells via indirect immunofluorescence, confirming effective lymphoma cell killing across isotypes.
Tags: anti-human HLA-DR/DP/DQ F3.3 in vivo; anti-human HLA-DR/DP/DQ F3.3 antibody in vivo
Evaluating antibodies for their capacity to induce cell-mediated lysis of malignant B cells
Stockmeyer, B., et al. (1998). Cancer Res. 1998 Jul 15;58(14):3051-4. PMID: 9679970
The F3.3 antibody (anti-human HLA-DR/DP/DQ) was tested for its ability to induce polymorphonuclear neutrophil (PMN)-mediated lysis in in vivo mouse models. HLA class II antibodies, including F3.3, consistently mediated high levels of target cell killing. In vivo experiments showed enhanced tumor regression with F3.3, and bispecific [HLA class II x FcyRI] antibodies significantly improved survival in lymphoma-bearing mice.
Tags: anti-human HLA-DR/DP/DQ F3.3 in animal model; anti-human HLA-DR/DP/DQ F3.3 mAb in animal model
Generation of HER-2/neu-specific cytotoxic neutrophils in vivo: efficient arming of neutrophils by combined administration of granulocyte colony-stimulating factor and Fcgamma receptor I bispecific antibodies
Heijnen, I. A., et al. (1997). J Immunol. 1997 Dec 1;159(11):5629-35. PMID: 9548506
The F3.3 antibody (anti-human HLA-DR/DP/DQ) was used with granulocyte colony-stimulating factor (G-CSF) to arm neutrophils in vivo, enhancing tumor cell killing. The combination of G-CSF and F3.3 bispecific antibodies led to specific cytotoxicity against HER-2/neu-expressing cells, demonstrating efficient neutrophil recruitment and activation in mouse models.
Tags: anti-human HLA-DR/DP/DQ F3.3 in cancer research; anti-human HLA-DR/DP/DQ F3.3 mAb in cancer research
HLA class II as potential target antigen on malignant B cells for therapy with bispecific antibodies in combination with granulocyte colony-stimulating factor
Stockmeyer, B., et al. (1996). Blood. 1996 May 1;87(9):3803-12. doi: 10.1182/blood.V87.9.3803.bloodjournal8793803. PMID: 8611706
HLA class II antibodies, including F3.3 (anti-human HLA-DR/DP/DQ), mediated significant cytotoxicity with neutrophils from healthy donors and G-CSF-treated patients against B-cell lines. Bispecific antibodies, such as [HLA class II x FcyRI] and [HLA class II x FcyRII], cross-linked with F3.3 F(ab’) fragments, achieved 15.7–33.9% specific lysis of RAJI cells at 10 pg/mL, demonstrating effective targeting of malignant B cells.
Tags: function of anti-human HLA-DR/DP/DQ F3.3; anti-human HLA-DR/DP/DQ F3.3 mAb of low endotoxin
For more references about anti-human HLA-DR/DP/DQ antibody (F3.3), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG1 isotype control antibody
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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