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Reverse Transcriptases

Moloney Murine Leukemia Virus Reverse Trancscriptase, RT Enzyme

Catalog No. Product Name Size List Price (US$) Quantity
MB036-5G 5G Reverse Transcriptase 10,000 units 160.00
MB036-4G 4G Reverse Transcriptase 10,000 units 160.00
MB036-MM M-MLV(H-) Reverse Transcriptase 10,000 units 96.00
Description

MB036-5G: 5G Reverse Transcriptase

The 5G Reverse Transcriptase, the major component of the 5G first strand cDNA synthesis kit with gDNA elimination function, is an upgraded version of the 4G Reverse Transcriptase, which can efficiently perform reverse transcription at 37°C. The 5G Reverse Transcriptase retains the thermal stability of the 4G Reverse Transcriptase. The temperature of reverse transcription can be increased to 50~55℃, so that one can avoid the inhibition of cDNA synthesis by RNA complex secondary structure, and effectively synthesize high-quality cDNA. The 5G Reverse Transcriptase has excellent continuous synthesis ability and super strong impurity tolerance.

Features of 5G Reverse Transcriptase:

* Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
* Super impurity tolerance: tolerant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
* Higher reverse transcription efficiency than 4G reverse transcriptase.

Components of 5G Reverse Transcriptase:

5G RT Buffer (5X).
5G reverse transcriptase (200 U/ul).

Storage:

The reverse transcriptases are stable for one year when stored in a constant temperature freezer at -20°C. After thawing, mix the components thoroughly before using. Frequent freezing and thawing is not recommended.

MB036-4G: 4G Reverse Transcriptase

The 4G Reverse Transcriptase, the major component of the 4G first strand cDNA synthesis kit with gDNA elimination function, is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase with significantly improved thermo-stability. The half-life of the 4G Reverse Transcriptase at 50℃ is >240 min. At 55℃, the RT enzyme can also be stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structure. The 4G Reverse Transcriptase has a improved template affinity and cDNA synthesis efficiency. The 4G Reverse Transcriptase is resistant to most RT-PCR inhibitors, and is suitable for long-fragment cDNA amplification (as long as 20 kb).

Features of 4G Reverse Transcriptase:

* Extensive template compatibility: compatible with various templates such as animals, plants, and viruses.
* Super impurity resistance: resistant to common impurities (ethanol, isopropanol, water balance phenol, guanidine isothiocyanate, humic acid).
* Long-fragment cDNA amplification (as long as 20 kb).

Components of 4G Reverse Transcriptase:

4G RT Buffer (5X).
4G reverse transcriptase (200 U/ul).

MB036-MM: M-MLV(H-) Reverse Transcriptase

The wild-type M-MLV (Moloney Murine Leukemia Virus) has the following activities: RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity; RNase H activity. Because RNase H activity can catalyze the degradation of RNA in the DNA/RNA hybrid strand, the template RNA may be degraded in the cDNA one-strand synthesis reaction. The M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutagenesis. Compared with the common mutants obtained by deleting the RNase H domain, the M-MLV (H-) Reverse Transcriptase retains the complete protein structure, so it has the same polymerase activity as the wild type, and can be used for longer cDNA synthesis and full-length cDNA library Construction. The M-MLV (H-) Reverse Transcriptase has strand displacement activity and can be used in 5'RACE.

Features of M-MLV(H-) Reverse Transcriptase:

* Point mutations eliminate RNase H activity to obtain long cDNA products ≤5 kb.
* Stable and reliable reverse transcription performance for RNA templates above 100 ng.
* For 5’-RACE reaction and cDNA library construction.

Components of M-MLV(H-) Reverse Transcriptase:

M-MLV(H-) RT Buffer (5X).
M-MLV(H-) reverse transcriptase (200 U/ul).

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR PreMix with dye (green, red, blue).
* 2x rapid PCR Master Mix with a green dye.
* 2x long PCR Master Mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT-PCR master mixes
* 2x qPCR master mixes
* RT-qPCR master mixes

PCR components and related products:
* dNTP mix, 10 mM each.
* dNTP mix, 25 mM each.
* Proteinase K powder.
* Proteinase K solution.
* Reverse Transcriptases.
* First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* PCR enhancer.

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