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Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The in vivo grade recombinant anti-human HLA class II DR/DQ Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells.
Clone: 9.3F10.
Isotype: Mouse IgG2a Kappa.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 9.3F10) specifically binds to human HLA class II DR/DQ.
Applications: ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human HLA class II DR/DQ protein.
Formulation: 0.2 μM filtered solution of 1x PBS.
Purity: >95% by SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.
Shipping: The in vivo grade recombinant anti-human HLA class II DR/DQ antibodies (clone of 9.3F10) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Crossreactivity of a human autoimmune TCR is dominated by a single human leukocyte antigen residue.
Newell, E., et al. (2014). J Clin Invest. 2014 Oct;124(10):4277-89. doi: 10.1172/JCI73688. PMID: 25225671
The study utilized anti-HLA-DR/DQ antibody (9.3F10) and anti-HLA-A2 (BB7.2) to assess the crossreactivity of the Ob.1A12 TCR in Jurkat cells stained with peptide/MHC tetramers and analyzed by flow cytometry. The Ob.1A12 TCR recognized multiple self-peptides presented by HLA-DR, with a single HLA residue playing a dominant role in this crossreactivity. In vivo experiments in HLA-DR-transgenic mice confirmed the autoimmune potential of the Ob.1A12 TCR, with TCR-transduced Jurkat cells co-cultured with antigen-presenting cells to measure activation.
Tags: anti-human HLA class II DR/DQ 9.3F10; anti-human HLA class II DR/DQ 9.3F10 mAb
Self-reactive human CD4 T cell clones form unusual immunological synapses.
Singh, R., et al. (2012). J Exp Med. 2012 Feb 13;209(2):335-52. doi: 10.1084/jem.20112027. PMID: 22291028
The study used anti-HLA-DR/DQ antibody (9.3F10) and anti-HLA-DR (L243) to block MHC class II and investigate synapse formation in self-reactive CD4 T cell clones in a humanized mouse model. Two-photon microscopy visualized synapse formation in lymph nodes, showing that 9.3F10 significantly reduced synapse stability and T cell activation. T cell receptor signaling was assessed by measuring calcium flux upon antigen recognition.
Tags: anti-human HLA class II DR/DQ 9.3F10 in vivo; anti-human HLA class II DR/DQ 9.3F10 in animal model
Interleukin 2-Antibody and Tumor Necrosis Factor-Antibody Fusion Proteins Induce Different Antitumor Immune Responses In Vivo.
Christ, O., et al. (2001). Clin Cancer Res. 2001 May;7(5):1385-97. PMID: 11350943
The study employed anti-HLA-DR/DQ antibody (clone 9.3F10), anti-CD80 (clone BB1), and anti-CD86 (clone IT2.2) for blocking experiments in SCID mice bearing human tumor xenografts. IL-2 and TNF fusion proteins were administered intravenously, with tumor growth monitored over 21 days. Flow cytometry confirmed HLA-DR/DQ expression on tumor cells, and the combination of IL-2 and TNF fusion proteins enhanced CD8+ T cell infiltration into tumors.
Tags: anti-human HLA class II DR/DQ 9.3F10 mAb in animal model; function of anti-human HLA class II DR/DQ 9.3F10
CD4-Positive and CD8-Positive Cytotoxic T Lymphocytes Contribute to the Control of Cryptococcus neoformans in the Lungs of Mice.
Kawakami, H., et al. (1999). Clin Diagn Lab Immunol. 1999 Mar;6(2):236-41. PMID: 10092809
C57BL/6 mice were injected intraperitoneally with 0.5 mg of anti-HLA-DR/DQ mAb (9.3F10) 1 day before and 2 days after Cryptococcus neoformans infection. The role of CD4+ and CD8+ T cells in controlling pulmonary infection was assessed, with flow cytometry quantifying T cell populations in lung tissues. Depletion of CD4+ or CD8+ T cells increased fungal burden, demonstrating their contribution to host defense against Cryptococcus neoformans.
Tags: anti-human HLA class II DR/DQ 9.3F10; anti-HLA class II DR/DQ clone 9.3F10
Human Mycobacterium tuberculosis-reactive CD4+ T-cell clones from healthy, latently infected individuals.
Demkowicz, A., et al. (1991). Infect Immun. 1991 Aug;59(8):2737-43. doi: 10.1128/iai.59.8.2737-2743.1991. PMID: 1879230
The study used 25 μg/ml of anti-HLA-DR/DQ antibody (9.3F10) or anti-HLA class I (W6/32) to preincubate B cells, confirming MHC class II-restricted antigen presentation. CD4+ T cell clones from healthy individuals with latent Mycobacterium tuberculosis infection were isolated, and proliferation assays verified their specificity to mycobacterial antigens. Flow cytometry confirmed MHC class II expression on antigen-presenting B cells, establishing that CD4+ T cell responses were restricted by HLA-DR/DQ molecules.
Tags: bioactivity of anti-human HLA class II DR/DQ 9.3F10; bioactivity of anti-human HLA class II DR/DQ 9.3F10 mAb
For more references about anti-human HLA class II DR/DQantibody (9.3F10), please contact our scientific support team with message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
In vivo grade recombinant mouse IgG2a isotype control antibody
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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