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Human IgG1 Isotype Control Antibody

Recombinant Human IgG1 Reference Antibody, Recombinant Human IgG1 Negative Control Antibody

Catalog No. Product Name Size List Price (US$) Quantity
PA007125 In Vivo Grade Recombinant Human IgG1 Isotype Control Antibody 1 mg 300.00
PA007125 In Vivo Grade Recombinant Human IgG1 Isotype Control Antibody 5 mg 1000.00
PA007125 In Vivo Grade Recombinant Human IgG1 Isotype Control Antibody 20 mg 2400.00
Description

PA007125: Recombinant Human IgG1 kappa Isotype Control Antibody

The recombinant human IgG1 kappa isotype control antibody was produced in CHO cells.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin Level: Less than 1 EU/ug of protein as determined by LAL method.

Shipping: The product is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 month from date of receipt, 2 to 8°C as supplied.
3 months from date of receipt, -20°C to -70°C as supplied.

Background

Isotype control antibodies (negative control antibodies or reference antibodies) are frequently used in various antibody-related in vivo and in vitro experiments such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Isotype control antibodies are used as negative controls of primary antibodies to check presence of non-specific binding to Fc receptors on the cell surface, other cellular proteins, carbohydrates, and lipids. Thus, the isotype control antibody normally matches the host species and isotype, including light chains (such as human IgG1 kappa antibody), concentration, and conjugation format of the experimental primary antibody. The ideal human IgG1 isotype control antibody has low or no specific binding to any human sample, and humanized or completely human variable regions in addition to the human IgG1 constant regions.

Most of the therapeutic antibody drugs carry the natural human IgG1 constant regions or engineered mutants. Changes to the IgG Fc sequence including glycosylation sites have been used to modulate Fc functions. Even a single mutation may have both enhancement and abrogation effects of Fc binding. The following mutations have been introduced to alter the constant regions of an IgG1 antibody to change its immunologic properties by Fc interaction with host proteins:

hIgG1 heavy chain R214K (Polatuzumab)
hIgG1 heavy chain R214K/N297A (Atezolizumab)
hIgG1 heavy chain L234A/L235A (Risankizumab)
hIgG1 heavy chain L234F/L235E/P331S (Durvalumab)
hIgG1 heavy chain L235A/G237A (Vedolizumab)
hIgG1 heavy chain D356E/L358M (Ocrelizumab)
hIgG1 heavy chain F126L (Necitumumab)
hIgG1 heavy chain D265A
hIgG1 heavy chain F243L/R292P/Y300L/V305I/P396L
hIgG1 heavy chain S239D/I332E
hIgG1 heavy chain S239D/I332E/A330L
hIgG1 heavy chain S298A/E333A/K334A
hIgG1 heavy chain G236A/S239D/I332E
hIgG1 heavy chain K326W/E333S
hIgG1 heavy chain S267E/H268F/S324T
hIgG1 heavy chain E345R/E430G/S440Y
hIgG1 heavy chain N297A
hIgG1 heavy chain N297Q
hIgG1 heavy chain N297G
hIgG1 heavy chain L235E
hIgG1 heavy chain L234A/L235A
hIgG1 heavy chain M252Y/S254T/T256E
hIgG1 heavy chain M428L/N434S
hIgG1 heavy chain S267E/L328F
hIgG1 heavy chain N325S/L328F

Recombinant natural and engineered human IgG1 isotype control antibody and mutants are available. Biotinylated hIgG1 isotype control antibodies, and hIgG1 isotype control antibodies with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered human IgG1 isotype control antibodies, biotinylated hIgG1 isotype control antibodies, and hIgG1 isotype control antibodies with Avi-, His-, and Flag-tags.

Other Recombinant IgG Isotype Control Antibodies:
Recombinant Human IgG2 Isotype Control Antibody
Recombinant Human IgG4-S228P Isotype Control Antibody
Recombinant Mouse IgG1 Isotype Control Antibody
Recombinant Mouse IgG2a Isotype Control Antibody
Recombinant Mouse IgG2b Isotype Control Antibody

Recombinant IgG Fc Proteins:
Recombinant Human Fc Protein (IgG1)
Recombinant Human Fc Protein (IgG2)
Recombinant Human Fc Protein (IgG4)
Recombinant Mouse Fc Protein (IgG1)

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit

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