The most comprehensive rabbit polyclonal antibody production services with the gold standard of rabbit immunization service (1 immunization and 4 boosts): custom production of rabbit polyclonal antibodies (pAb) against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; rabbit polyclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.
The highest quality standard in the field of rabbit polyclonal antibody production services: guaranteed ELISA > 1:64,000 (antisera) and > 1: 128,000 (purified antibodies).
Fast turnaround: 10 weeks once the antigen is available.20000 cells per hybridoma clone are sufficient for cloning and sequencing the monoclonal antibody variable regions (both VH and VL) and full length. Welcome to challenge us with smaller number of clonal B cells or non-viable cells for hybridoma rescue.
Both Sanger sequencing-based and nanopore sequencing-based methods are available for antibody sequencing from hybridoma and clonal B cells. Sanger sequencing is labor intensive and time consuming so that it is difficult to reduce the cost for a large number of samples. However, nanopore sequencing can be used to reduce the cost signicantly, especially for a large number of samples.
Single B cell antibody sequencing has become popular for antibody discovery, especially for those difficult to obtain. After antigen specific single B cell sorting, the single B cells can be directly used for antibody seqencing or cultured for 2 weeks before antibody sequencing. Single B cell sequencing by next generation sequencing (NGS) including nanopore sequencing has been successfully applied to single B cell antibody technologies.
Syd Labs has extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized secretion signal and linker sequences, Syd Labs can efficiently complete a project from DNA construction and transient production of Fc fusion proteins in CHO and HEK 293 cells, to Fc fusion protein purification. N-term or C-term of Fc fusions of peptides and proteins in secreted form and multiple Fc backbone are available upon request.
BS050B:
Synthesis of the gene encoding the Fc fusion protein;
Molecular construction of the recombinant Fc fusion protein;
0.04 Liter transient production of the recombinant Fc fusion protein in CHO or HEK293 cells;
Protein A purification.BS046B:
Gene synthesis of the variable regions of the recombinant IgG antibodies;
Molecular construction of the recombinant IgG antibodies;
0.04 Liter transient production of the recombinant IgG antibodies in CHO cells;
Protein A purification.BS079B:
Gene synthesis of the variable regions of the recombinant scFv-Fc antibodies;
Molecular construction of the recombinant scFv-Fc antibodies;
0.04 Liter transient production of the recombinant scFv-Fc antibodies in CHO cells;
Protein A purification.Orchestrating different elements essential for optimal mammalian gene expression.
Fast return: 2-3 weeks.
Having made thousands of mammalian expression constructs and tested them in cell culture and in vivo, we are highly experienced in designing expression constructs for superb expression.Fast return: 4 days for ligation subcloning and additional 4 days if PCR is required;
The insert and vector sizes do not matter;
Various DNA templates;
Vectors which you want to subclone DNA fragments into.The most comprehensive rat monoclonal antibody development services: custom production of rat monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.
The highest quality standard in the field of rat monoclonal antibody development services: guaranteed ELISA > 1:40,000.
Choice for licensing antibodies to save your cost and even make money.
Fast turnaround: 24 weeks once the antigen is available.The most comprehensive hybridoma-based mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins as well as small molecular compounds; for ELISA, Western blot, IHC, and flow cytometry applications.
The lowest custom mouse monoclonal antibody production price: $6000.
Fast turnaround: 24 weeks once the antigen is available.