BS079B-14-05:
Gene synthesis of the variable regions of the recombinant scFv-Fc antibodies;
Molecular construction of the recombinant scFv-Fc antibodies;
0.1 Liter transient production of the recombinant scFv-Fc antibodies in CHO cells (14-day fed-batch cell culturing);
At least 2-step antibody protein purification;
Quality guarantee: SDS-PAGE >95%, SEC-HPLC >95%, and endotoxin <0.05 EU/mg. Mass spectrometry may be used to check molecular weight of the purified antibody.Syd Labs has extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized secretion signal and linker sequences, Syd Labs can efficiently complete a project from DNA construction and transient production of Fc fusion proteins in CHO and HEK 293 cells, to Fc fusion protein purification. N-term or C-term of Fc fusions of peptides and proteins in secreted form and multiple Fc backbone are available upon request.
BS050B-14-05:
Synthesis of the gene encoding the Fc fusion protein;
Molecular construction of the recombinant Fc fusion protein;
0.1 Liter transient production of the recombinant Fc fusion protein in CHO cells (14-day fed-batch cell culturing);
At least 2-step antibody protein purification;
Quality guarantee: SDS-PAGE >95%, SEC-HPLC >95%, and endotoxin <0.05 EU/mg. Mass spectrometry may be used to check molecular weight of the purified antibody.BS046B-14-05:
Gene synthesis of the variable regions of the recombinant IgG antibodies;
Molecular construction of the recombinant IgG antibodies;
0.1 Liter transient production of the recombinant IgG antibodies in CHO cells (14-day fed-batch cell culturing);
At least 2-step antibody protein purification;
Quality guarantee: SDS-PAGE >95%, SEC-HPLC >95%, and endotoxin <0.05 EU/mg. Mass spectrometry may be used to check molecular weight of the purified antibody.Orchestrating different elements essential for optimal mammalian gene expression.
Fast return: 2-3 weeks.
Having made thousands of mammalian expression constructs and tested them in cell culture and in vivo, we are highly experienced in designing expression constructs for superb expression.Fast return: 4 days for ligation subcloning and additional 4 days if PCR is required;
The insert and vector sizes do not matter;
Various DNA templates;
Vectors which you want to subclone DNA fragments into.The most comprehensive rat monoclonal antibody development services: custom production of rat monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.
The highest quality standard in the field of rat monoclonal antibody development services: guaranteed ELISA > 1:40,000.
Choice for licensing antibodies to save your cost and even make money.
Fast turnaround: 24 weeks once the antigen is available.The most comprehensive hybridoma-based mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins as well as small molecular compounds; for ELISA, Western blot, IHC, and flow cytometry applications.
The lowest custom mouse monoclonal antibody production price: $6000.
Fast turnaround: 24 weeks once the antigen is available.De novo antibody sequencing to obtain the intact amino acid sequence of variable and/or constant regions of monoclonal antibodies by LC-MS/MS.
Sample requirement: 3-5 mg at 1 mg/ml preferred (minimum: 2 mg at 1 mg/mL).
Fast turnaround time: 2-3 months per antibody, but may require more time depending on the complexity of analysis.BS052: HRP Conjugation Service
1. Highly efficient process to conjugate HRP to clients' proteins and antibodies
2. Amine-reactive HRP achieves the highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. HRP enzyme activity is ~150 units/mg
5. Fast turnaround time: 3-5 daysBS077: Fluorophore Conjugation Service
1. Highly efficient process to conjugate all commercially available fluorophore to clients' proteins and antibodies
2. The highest possible conjugation yield and stability
3. Final product can be stored for at least 12 months at -20 C.
4. Fast turnaround time: 3-5 days