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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Cheapest alkaline-stable protein A resin with high dynamic binding capacity: ≥ 55 mg/mL (human IgG, 4 minute residence time). Clean in Place: 0.1-0.5 M NaOH. Recombinant alkaline-stable protein A resins are looking for distributors.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
The alkaline-stable protein A resin carries the engineered recombinant protein A ligand immobilized onto our proprietary agarose base matrices to provide cost-effective solutions for 1) fast-processing of monoclonal and polyclonal antibodies, bispecific antibodies, and Fc fusion constructs with high quality from biological fluids and cell culture media; and 2) immunoprecitation of proteins and protein complexes.
IA021: NMab Protein A Resin
The NMab protein A resin offers end users cost competitive and high perfomance Protein A affinity resins. The NMab protein A resin is an agarose based Protein A resin developed using engineered alkaline-stable recombinant protein A ligand and proprietary technologies of agarose crosslinking, surface extension, and ligand coupling. The NMab protein A resin provides the combination of excellent binding capacity, low non-specific binding that leads to low impurity levels in the elution pool, good pressure-flow properties, and superior alkaline-tolerance for stability in the alkaline conditions used in Cleaning-in-place (CIP) and sanitization protocols. Table 1 lists the basic characteristics of this protein A resin:
Characteristics: Specification
Separation Function: Protein A affinity
Matrix: Cross-linked agarose
Ligand: Alkaline-stabilized recombinant protein A
Coupling Chemistry: Epoxy
Particle Size（μm): average 90 μm (range 70-120 μm)
Dynamic Binding Capacity: ≥ 55 mg/mL (human IgG, 4 minute residence time)
Protein A Leachate: < 20 ng/ml
Maximum Pressure: 0.3 MPa
Clean in Place: 0.1-0.5 M NaOH
Recommended Flow Velocity: 100-500 cm/hour
pH Stability: 2-12
Chemical Stability: Stable in commonly used buffers, 0.1 M HAc, 0.1 M HCl, 100% ethanol, isopropyl alcohol, etc. Avoid long-term exposure to strong acids or strong bases.
Operational Temperature: 2-40 ℃
Storage: 20% Ethanol or benzyl alcohol, 2-8 ℃
The engineered protein A in NMab protein A resin is manufactured with animal free raw materials.
NMab protein A resin provides reliable all around performance, low protein A leakage, high binding capacity and a useful effective working range requiring a residence time of at least 3 minutes (equivalent to a typical running speed of 300 cm/hr). It is available in bottled (20% ethanol at 60% slurry concentration) or pre-packed column packaging.
For comparison, please see:
NMab Pro protein A resin
UniMab 50 HC Protein A resin
NMAB protein A resin, which combines the agarose bead and the engineered protein A ligand using proprietary technologies of agarose crosslinking, surface extension, and ligand coupling, is the major component in pre-packed protein A columns.
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