Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
No DNAs using our Fc fusion protein expression vectors will be delivered.
All yields are estimates and not guaranteed.
Prices of BS050C to BS050H do not include DNA cloning and scale-up.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Syd Labs has extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized secretion signal and linker sequences, Syd Labs can efficiently complete a project from DNA construction and transient production of Fc fusion proteins in CHO and HEK 293 cells, to Fc fusion protein purification. N-term or C-term of Fc fusions of peptides and proteins in secreted form as well as multiple Fc backbone are available upon request.
The Fc region (the antibody fragment crystallizable region) brings a variety of benefit to the fused proteins and peptides. Fc fusion allows simple and efficient purification with protein A and/or protein G, and accurate quantification and capturing in receptor-ligand affinity measurement. Fc fusion significantly improves pharmacokinetic property of the fused proteins and peptides because Fc can escape proteolytic degradtion. Fc can increase the plasma half-life of Fc fusion proteins due to interaction with the neonatal Fc receptor (FcRn) and the slowing of renal clearance due to their larger size. Because they are homodimers, Fc fusion proteins may have improved apparent affinity to binding partners due to increased avidity. Because many receptors are only functional in a dimeric form, the dimeric receptor-Fc fusion protein can mimic the activated form and enhance its affinity for the cognate ligand. With human-specific glycosylation, Fc chimeric proteins can be used in studies involving human proteins and cells. They can be used to neutralize the bioactivity of their cognate ligands in vitro and in vivo.
1. Synthesizing or amplifying the gene encoding the Fc-fused protein:
Free sequence optimization to meet your goals such as recombinant Fc-fusion protein expression at high and reliable levels.
2. Construction, expression, and purification of Fc-fusion proteins:
* Fuse your protein of interest into human or mouse IgG Fc backbone (choice of human or mouse IgG1 Fc backbone; other IgG Fc backbone is available upon request) in mammalian expression vectors;
* Transiently express the recombinant Fc-fusion protein in mammalian expression systems, CHO or HEK 293 cells;
* Purify the recombinant Fc-fusion protein. Protein A is used for recombinant Fc-fusion protein purification. Please request if multiple purification methods are required.
The Fc options include but are not limited to:
* Human IgG1-Fc, IgG2-Fc, IgG3-Fc, IgG4-Fc;
* Mouse IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc;
* Rat IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc;
* Rabbit IgG-Fc;
* Canine IgG-Fc.
If you provide us the Fc fusion expression vector with your gene already cloned into, we perform small-scale pilot expression and protein purification by transient transfection into CHO or 293 cells, allowing us to evaluate expression level and yield of the Fc fusion protein of your interest.
Fast turnaround (BS050A):
* 1-2 weeks for codon optimization and synthesizing the gene of interest.
* 1-2 weeks for molecular construction of recombinant Fc fusion proteins using E.coli.
For expressing recombinant Fc fusion proteins using mammalian expression systems, CHO or HEK 293 cells:
BS050C: 0.03 liter transient production, estimated yield 0.5 - 20 mg, average 5 mg, 2 weeks;
BS050D: 0.1 liter transient production, estimated yield 2 - 50 mg, average 10 mg, 2 weeks;
BS050E: 0.5 liter transient production, estimated yield 10 - 250 mg, average 50 mg, 2 weeks;
BS050F: 2 liter transient production, estimated yield 40 - 1000 mg, average 200 mg, 3 weeks;
BS050G: 10 liter transient production, estimated yield 200 - 5000 mg, average 1000 mg, 3 weeks.
We also provide the following protein production (DNA construction, protein expression and purification) services:
Albumin Fusion Protein Production Service
His Tag or FLAG Tag Fusion Protein Production Service
Applications:
ScFv-Fc fusion protein production: molecular construction of recombinant single chain antibodies (scFv-Fc), 0.03 liter transient production of recombinant scFv-Fc in CHO or HEK 293 cells, and protein A purification of recombinant scFv-Fc
Recombinant IgG Fc Proteins:
Recombinant Human Fc Protein (IgG1)
Recombinant Human Fc Protein (IgG2)
Recombinant Human Fc Protein (IgG4)
Recombinant Mouse Fc Protein (IgG1)
Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit
Protein A, Resin, Columns, and ELISA Kits:
Recombinant Protein A
Protein A Affinity Resin
Pre-packed Protein A Column
Protein A ELISA Kit
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