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Rabbit Polyclonal Antibody Production Services

Rabbit Polyclonal Antibody Development, Custom Rabbit Polyclonal Antibody Production

Catalog No. Product Name Size List Price (US$) Quantity
BS014A Standard Rabbit Polyclonal Antibody Production Service 1 package 1200.00
BS014B Standard Rabbit Polyclonal Antibody Production Service + Standard Peptide Synthesis + Peptide Conjugation 1 package 1600.00
BS014C Standard Rabbit Polyclonal Antibody Production Service + Standard Peptide Synthesis + Peptide Conjugation + Antigen Affinity purification 1 package 2100.00
BS014D Standard Rabbit Polyclonal Antibody Production Service + DNA Subcloning + Bacterial Expression and Purification of Protein Antigen 1 package 4400.00
BS014E Standard Rabbit Polyclonal Antibody Production Service + DNA Subcloning + Bacterial Expression and Purification of Protein Antigen + Protein A Affinity Purification 1 package 4800.00
Description

The most comprehensive rabbit polyclonal antibody production services: custom production of rabbit polyclonal antibodies (pAb) against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; rabbit polyclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications.

With experience of producing thousands of rabbit polyclonal antibodies each year, Syd Labs team and partners found two key factors to rabbit polyclonal antibody generation: good immunogen; more immunization times. Although the cost increases with 4 boosts rather than 2 boosts, Syd Labs guarantee the best polyclonal antibodies to clients.

BS014A: $450 for rabbit polyclonal antibody development following the best immunization protocol (2 rabbits, 1 immunization and 4 boosts per rabbit)

You provide: Ten mg of peptide (purity > 85%, length > 12 amino acids) or 4 mg of soluble protein (concentration > 0.4 mg/ml, purity > 90%, molecular weight > 20 kDa) for immunization, and additional 5 mg for antigen affinity purification.

Deliverable:

* 30-50 ml of antisera/rabbit;
* Remaining antigens;
* ELISA results.

The highest quality standard in the field of rabbit polyclonal antibody production services: guaranteed ELISA > 1:64,000 (antisera) and > 1: 128,000 (purified antibodies).

The prices will be adjusted for the following requirements:

a. Peptide (12-15 amino acids, >85%) design and synthesis +$150 for standard, +$450 for mono-phosphorylated, +$650 for di-phosphorylated, +$700 for mono-methylated (K or R site), +$650 for di-methylated (K or R site), +$700 for tri-methylated (K site), +$400 for acetylated;
b. Polyclonal antibody production service from peptide synthesis to antibody affinity purification for modified peptide: +$600 for phospho-specific;
c. Synthesis of other antigens, +$variable;
d. Conjugation of antigens to protein carriers +$100;
e. Protein: DNA subcloning +$350, bacterial expression and purification +$2,000;
f. Additional boost immunization for higher ELISA titer of the antisera +$100/injection;
g. Additional animal +$250/rabbit;
h. Antibody purification: antigen affinity purification +$350, protein A/G affinity purification +$250, ammonium sulfate precipitation +$200;
i. Guaranteed applications: Western blot +$2,000, IHC +$2,500.

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