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Hot Start Taq DNA Polymerase

Hot-Start DNA Polymerase, Hotstart Taq DNA Polymerase

Catalog No. Product Name Size List Price (US$) Quantity
MB087 Hot Start Taq DNA Polymerase, dNTP included 500 units 108.00
MB087 Hot Start Taq DNA Polymerase, dNTP included 5000 units 880.00
MB117 Hot Start Taq DNA Polymerase for Probe-based qPCR, dNTP included 500 units 108.00
MB117 Hot Start Taq DNA Polymerase for Probe-based qPCR, dNTP included 5000 units 880.00
Description

MB087: Hot Start Taq DNA Polymerase

The hot start Taq DNA polymerase is a mixture of Taq DNA polymerase and a Taq antibody in an optimal ratio. Due to the unique thermo stability of the Taq antibody, the activity of the hot start Taq DNA polymerase is blocked at temperature up to 55℃, which minimizes non-specific amplification during the mixing and system heating. After 30 seconds at 95℃, the Taq antibody can be completely inactivated so that the Taq enzyme activity is completely released, ensuring that the PCR reaction has extremely high amplification sensitivity and specificity. The activation of the hot start Taq DNA polymerase is not affected by pH, ionic strength, and so on. It is used in various hot-start PCR and qPCR, and can be used to amplify genes with low copy numbers from complex templates such as genomic DNAs and cDNAs. The product is a good choice of the hot-start Taq enzyme for PCR/qPCR-based molecular diagnostic reagents. It has higher stability and detection rate than the chemical-based hot start Taq DNA polymerase. The 3' end nucleotide of the PCR product is A, so the PCR fragment can be cloned into the T-vector. It is suitable for TOPO cloning kit and our homologous recombination-based one step DNA subcloning kit.

Unit definition: One unit of the hot start Taq DNA polymerase means incorporating 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74°C.

Concentration: 5 units/ul.

The PCR reagent concentrations, reaction conditions, and PCR program parameters should be determined experimentally by the investigator.

Features of the hot start Taq DNA polymerase:
* High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 5 kb.
* High specificity.
* Reproducible results.
* Terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product, facilitating TA cloning of PCR products.
* Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.

Applications of the hot start Taq DNA polymerase:
* Routine PCR using genomic, viral and plasmid templates.
* RT-PCR and RT-qPCR.
* DNA cloning and subcloning.
* SYBR green-based real time PCR (qPCR or quantitative PCR).
* Taqman probe-based real time PCR (qPCR or quantitative PCR).
* DNA mutagenesis.
* Generating hybridization probes for Southern or Northern hybridization.

The cheap hotstart PCR enzyme is also available in the cheap 2X hot-start PCR PreMix, with dye (catalog No. MB067-EQ2).

Please contact us if you need protocol, datasheet, or SDS of our hot start Taq DNA polymerase.

Quality of our hot start Taq DNA polymerase has been proven by a number of publications citing our product.

MB117: Hot Start Taq DNA Polymerase for Probe-based qPCR

The hot start Taq DNA polymerase for probe-based qPCR is a mixture of Taq DNA polymerase and a Taq antibody in an optimal ratio. The antibody included has higher affinity to the Taq enzyme. The hot start Taq DNA polymerase for probe-based qPCR significantly improves amplification specificity and sensitivity for detection of low-copy genes paired with the most suitable buffer optimized for qPCR and PCR. This product is suitable for probe-based qPCR detection of DNA templates including DNA viruses. The hot start Taq DNA polymerase for probe-based qPCR provides excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes. The product has wide compatibility in terms of template type, template GC content, and primer Tm values. It has good tolerance to impurities and is suitable for use in a variety of testing scenarios.

Unit definition: One unit of the hot start Taq DNA polymerase means incorporating 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74°C.

Concentration: 5 units/ul.

The PCR reagent concentrations, reaction conditions, and PCR program parameters should be determined experimentally by the investigator.

Features of the hot start Taq DNA polymerase:
* High amplification specificity and sensitivity for detection of low-copy genes paired with the most suitable buffer optimized for qPCR and PCR.
* For probe-based qPCR, excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes.
* Compatibility in terms of template type, template GC content, and primer Tm values.
* Good tolerance to impurities and is suitable for use in a variety of testing scenarios.
* Low cost: $0.19+ per unit. The cheapest hot start Taq enzyme in the market.

Applications of the hot start Taq DNA polymerase:
* Routine PCR using genomic, viral and plasmid templates.
* RT-PCR and RT-qPCR.
* DNA cloning and subcloning.
* SYBR green-based real time PCR (qPCR or quantitative PCR).
* Taqman probe-based real time PCR (qPCR or quantitative PCR).
* DNA mutagenesis.
* Generating hybridization probes for Southern or Northern hybridization.

PCR enzymes:
* Taq DNA polymerase.
* High yield Taq DNA polymerase.
* Hot-start high-fidelity DNA polymerase.
* Hot start Taq DNA polymerase with chemical or antibody inhibitor, used for SYBR green or Taqman probe-based real time PCR (qPCR or quantitative PCR).

2x PCR master mixes:
* 2x PCR PreMix with dye (green, red, blue).
* 2x rapid PCR Master Mix with a green dye.
* 2x long PCR Master Mix with a blue dye.
* 2x high yield PCR master mix with a blue dye.
* 2x hot-start high-fidelity PCR master mix with a blue dye.
* Blood direct PCR kit
* Cell and tissue direct PCR kit
* RT-PCR master mixes
* 2x qPCR master mixes
* RT-qPCR master mixes

PCR components and related products:
* dNTP mix, 10 mM each.
* dNTP mix, 25 mM each.
* Proteinase K powder.
* Proteinase K solution.
* Reverse Transcriptases.
* First strand cDNA synthesis kits.
* Murine RNase inhibitor.
* PCR enhancer.

Questions and Answers:

Question: Do you provide free sample of your hot start Taq DNA polymerase?
Answer: Sorry that we do not provide free sample. You can place an order and we will hold the invoice until you tell us that you like our product. You do not need to pay us if you do not like the product. It is an alternative to free sample. 

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