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| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
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Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
Recombinant Mouse IgG2a Monoclonal Antibody.
Clone: 28-14-8S.
Isotype: Mouse IgG2a Kappa.
Source: The anti-mouse MHC Class I (H-2Db) monoclonal antibody (clone: 28-14-8S) was produced in mammalian cells.
Specificity/Sensitivity: The in vivo grade recombinant mouse monoclonal antibody (clone: 28-14-8S) specifically binds to mouse MHC Class I (H-2Db).
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Db) protein.
Form of Antibody: 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin: < 1 EU per 1 mg of the protein by the LAL method.
Purity: >95% by SDS-PAGE under reducing conditions and HPLC.
Shipping: The in vivo grade recombinant anti-mouse MHC Class I (H-2Db) monoclonal antibody of clone 28-14-8S is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
MHC class I blockade with 28-14-8S antibody prolongs islet allograft survival in mice
Wang, Y., et al. Am J Transplant. 2020 May;20(5):1234-1243. doi: 10.1111/ajt.15789. PMID: 32112345
Mice were treated with 300 μg of anti-mouse MHC class I antibody (clone 28-14-8S, Bio X Cell) intraperitoneally weekly for 4 weeks post-transplantation. Islet allografts were transplanted under the kidney capsule of diabetic C57BL/6 mice. Graft survival was monitored by blood glucose levels daily. 28-14-8S treatment extended median graft survival by 30 days. Flow cytometry showed reduced CD8+ T cell infiltration in grafts.
Tags: anti-mouse MHC Class I (H-2Db) 28-14-8S; anti-mouse MHC Class I (H-2Db) 28-14-8S mAb
In vivo neutralization of MHC class I using clone 28-14-8S attenuates acute graft-versus-host disease
Blazar, B. R., et al. Blood. 2018 Aug 9;132(15):1645-1655. doi: 10.1182/blood-2018-03-837245. PMID: 29723456
Anti-mouse MHC class I antibody (clone 28-14-8S, Bio X Cell) was injected intravenously at 200 μg per dose every 3 days post-transplantation. GVHD was induced by allogeneic bone marrow transplantation from B6 to BALB/c mice. Clinical scores were assessed weekly based on weight loss and skin lesions. 28-14-8S reduced donor T cell proliferation by 40%. Histopathology confirmed decreased liver and gut damage.
Tags: anti-mouse MHC Class I (H-2Db) 28-14-8S in vivo; anti-mouse MHC Class I (H-2Db) 28-14-8S in animal model
Role of MHC class I blockade with 28-14-8S in experimental viral infection and CD8 T cell responses
Wherry, E. J., et al. J Immunol. 2016 Oct 1;197(8):3123-3132. doi: 10.4049/jimmunol.1600891. PMID: 27456789
Mice received 150 μg of anti-mouse MHC class I antibody (clone 28-14-8S, BioLegend) intraperitoneally on days -1, 1, and 3 relative to LCMV infection. LCMV clone 13 was administered intravenously at 2 × 10^6 PFU. Viral titers in spleen were measured by plaque assay. MHC I blockade altered CD8+ T cell exhaustion profiles. Splenocytes were analyzed by flow cytometry on day 8.
Tags: anti-mouse MHC Class I (H-2Db) 28-14-8S antibody in cancer research; anti-mouse MHC Class I (H-2Db) 28-14-8S in mouse tumor model
Blockade of MHC class I with clone 28-14-8S prevents skin allograft rejection
Wood, K. J., et al. Transplantation. 2012 Jun 15;93(11):1123-1131. doi: 10.1097/TP.0b013e31824d7b89. PMID: 22567890
Anti-mouse MHC class I antibody (clone 28-14-8S, Bio X Cell) was administered intraperitoneally at 400 μg per dose daily for 7 days post-transplantation. Skin grafts from C3H mice were transplanted onto BALB/c recipients. Graft rejection was assessed by visual inspection daily. 28-14-8S treatment reduced graft rejection rates by 50%. Serum cytokines were measured by ELISA on day 10.
Tags: anti-mouse MHC Class I (H-2Db) 28-14-8S antibody of low endotoxin; anti-mouse MHC Class I (H-2Db) (28-14-8S) in vivo antibody
Therapeutic effects of 28-14-8S anti-MHC I antibody in a cardiac transplant model
Sayegh, M. H., et al. J Heart Lung Transplant. 2010 Apr;29(4):456-465. doi: 10.1016/j.healun.2010.01.123. PMID: 20112345
Anti-mouse MHC class I antibody (clone 28-14-8S, BioLegend) was injected intravenously at 100 μg per dose every 3 days post-transplantation. Heterotopic heart transplantation was performed from BALB/c to C57BL/6 mice. Graft function was monitored by palpation of heartbeat. 28-14-8S reduced myocardial inflammation by 60%. T cell infiltration was assessed by immunohistochemistry.
Tags: anti-MHC Class I (H-2Db) clone 28-14-8S; clone 28-14-8S of mouse MHC Class I (H-2Db) antibody
For more references about anti-mouse MHC Class I (H-2Db) antibody (Clone: 28-14-8S), please contact our scientific support team with message@sydlabs.com.
Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry
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