C019P: BeneFIX Biosimilar, Recombinant Factor IX
The yield of BeneFIX biosimilar from the CHO stable cell line was above 20 IU/mL in a 15 liter manufacturing.
C013P: Aflibercept Biosimilar
Recombinant protein composed of the binding domains of two human vascular endothelial growth factor (VEGF) receptors fused with the Fc region of human immunoglobulin gamma 1 (IgG1).
Specificity/Sensitivity: The aflibercept biosimilar protein specifically binds to the human VEGF.
Applications: Functional assays, and those assays for studying biological pathways affected by aflibercept.C018: Advate / Kogenate Biosimilar Stable Cell Line
The yield of Advate / Kogenate Biosimilar from the CHO stable cell line was above 60 IU/ml in a 50 liter manufacturing.
BP000027-GD1: Recombinant Human VEGF165 (aa 27-191) Protein
Source: HEK 293 cell-derived.
The VEGF activity is measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 1-5 ng/ml.
Endotoxin: < 1.0 EU per ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions.BP000028-GD2: Recombinant Human Long R3-IGF-I Protein
Source: E. coli-derived.
The IGF-1 activity is calculated by the dose-dependant proliferation of murine BALBC 3T3 cells. The ED50 for this effect is less than 1 ng/ml, corresponding to a specific activity of 1.0x10^6 IU/mg.
Purity: > 95% by SDS-PAGE and RP-HPLC.BP000029-GD3: Recombinant Human FGF Acidic 136 (aa 19-155) Protein
Source: E. coli-derived.
The FGF acidic (aFGF/FGF-1/FGF1) activity is measured in a NIH/3T3 cell proliferation assay. The ED50 for this effect is typically 0.1-0.3 ng/ml in the presence of 10 ug/ml of heparin.
Endotoxin: <1.0 EU per 1 ug of the protein by the LAL method.
Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.BP000030-GD4: Recombinant Human FGF Basic 147
Source: E. coli-derived.
The FGF basic (bFGF/FGF-2/FGF2) activity is measured in a cell proliferation assay using NIH-3T3 mouse fibroblasts. The ED50 for this effect is less than 0.5 ng/ml.
Endotoxin Level: <1.0 EU per 1 ug of the protein by the LAL method.
Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.BP000031-GD5: Recombinant Heparinase I from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >100 IU/mg. One international unit (IU) of recombinant Heparinase I is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0.
Purity >95% by SDS-PAGE.BP000032-GD6: Recombinant Heparinase II from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >6 IU/mg. One international unit (IU) of recombinant Heparinase II is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparin or heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.BP000033-GD7: Recombinant Heparinase III from Flavobacterium heparinum
Source: E. coli-derived.
Specific activity >50 IU/mg. One international unit (IU) of recombinant Heparinase III is defined as the amount of enzyme that will liberate 1.0 umole unsaturated oligosaccharides from heparan sulfate per minute at 30°C and pH 7.3.
Purity >95% by SDS-PAGE.